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Methods Proteins existed in adult Schistosoma japonicum membrane were extracted by Triton X-100 and purified through reverse-phase high performance liquid chromatography,and the main protein peaks were then collected separately. 125 I-LDL of human plasma as the ligand, through the radioautography and radioligand binding assay, the protein which can bind human serum 125 I-LDL specifically was identified.

自人工感染家兔的肠系膜下静脉取日本血吸虫成虫,用非离子去污剂TritonX 10 0抽提出日本血吸虫成虫表膜蛋白,反相高效液相色谱进行分离纯化后,收集各主要分离蛋白峰,以12 5I标记的人血浆低密度脂蛋白作为放射性配体,经放射性自显影及放射性受体配体结合分析鉴定能与人血浆12 5I LDL特异结合的蛋白。

Lymphadenopathy inpatients with connective tissue diseases does not tend to cavitate,and as a result, these nodes are of uniform soft-tissue attenuationand demonstrate homogeneous enhancement following intravenouscontrast material administration.

有肠系膜淋巴结病的结缔组织病病人,其受累得淋巴结不向空洞化的发展,因此这些淋巴结呈均匀一致的软组织密度影,静脉注入造影剂后,它们常均匀强化。

Methods Modification I:Transverse incision was chosen on epigastirc,a piece of about 3×5 cm fascil strip with pedical as well as bilateral anterior and posterior sheath of recus and peritoneum was pulled through transverse mesocolon functioning as the supporting stick of traditional loop ostomy.The colon of ostomy was buried subcutaneously,stoma was opened instantly or secondarily depending on the patients′s condition.

一、选择上腹部正中线横向切口,将腹白线两旁的腹直肌前、后鞘连同腹膜切成3×5cm的带蒂筋膜条穿过横结肠系膜并缝合固定,替代传统袢式造瘘的支撑棒,造瘘肠段埋于皮下,根据病情需要直接或二期开放造瘘口,余手术操作基本同传统袢式造瘘术。

After administration of L-N〓-nitro-Arginine, a specific antagonist of NOS, it was found that the basal and stimulated NO release of blood vessels (aorta, internal carotid, renal and mensenteric artery) was decreased further. The increase of coronary blood flow stimulated by Ach was also reduced further, while the platelet aggregation degree and erythrocyte aggregation index increased significantly. Pathological examination indicated that aortic endothelium was destroyed, the blood vessels in cerebral, cardiac and renal tissues were hypertrophied further and some were almost occluded. The above fin dings resulted in SHR feeded with L-NNA having high incidence of stroke rate and high degree of renal cirrhosis when compared to control SHR. The infarct and hemorrhagic focuci in cerebral tissues, infarct focuci in cardiac tissues, atrophy and hyalinedegeneration of renal glomeruli and degeneration even necrosis of renal tubuli in renal tissues were al so found. The increase ratio of brain and heart to body weight and decrease ratio of kidney to body wei ght further demonstrated that the cerebral, cardiac and renal injuries were aggravated.

在上述结果的基础上进一步给予SHR NOS特异性拮抗剂左旋亚硝基精氨酸后,血管(胸主、颈内、肾及肠系膜动脉)NO基础释放及激动剂刺激释放进一步减少,Ach刺激冠脉流量的增加和胸主动脉内膜NOS活性也进一步减少,血小板聚集程度及红细胞聚集指数显著上升,病理切片显示胸主动脉内皮细胞破坏明显,脑心肾组织内的血管进一步增厚甚至几乎阻塞,导致饲L-NNA的SHR缺血性及出血性脑卒中发生率显著高于对照SHR,脑体重比显著升高;心肌内出现明显的缺血梗塞疤痕灶,心体重比显著升高;肾小球明显萎缩、玻璃样变性,肾小管也变性甚至坏死,使肾硬化程度显著增加呈颗粒性固缩肾,肾体重比也显著减少。

Methods Ten rabbits are put into an icebox which had been made adjustment to a low temperature (-10±2℃),and inbreathed a mixture gas made of 8.5% oxygen and 91.5% nitrogen.

家兔10只,置于低温(-10±2℃)的冰柜中,并给吸入含氧量为8.5%的氧/氮混合气体,30min后迅速观察肠系膜的微血管口径和血流速度的变化。

Methods Ten rabbits are put into an icebox which had been made adjustment to a low temperature (-10±2℃),and inbreathed a mixture gas made of 8.5% oxygen and 91.5% nitrogen.After 30min,the mice were taken out,and we quickly observed the changes of the caliber of mesentery microvessel and blood flow velocity.

家兔10只,置于低温(-10±2℃)的冰柜中,并给吸入含氧量为8.5%的氧/氮混合气体,30min后迅速观察肠系膜的微血管口径和血流速度的变化。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜,用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养,选取生长情况良好的传至第4代的人EG细胞,移植到急性心肌梗死大鼠的梗死心肌周围,分别于移植后1天、1、2、4周处死大鼠,以鼠抗人细胞核抗体MAB1281作为示踪剂,通过免疫组化方法检测移植细胞的分布。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜,用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养,选取生长情况良好的传至第4代的人eg细胞,移植到急性心肌梗死大鼠的梗死心肌周围,分别于移植后1天、1、2、4周处死大鼠,以鼠抗人细胞核抗体mab1281作为示踪剂[1],通过免疫组化方法检测移植细胞的分布。

Primordial germ cells derived from gonadal ridges and mesenteries of 30-70-day post menopause human ernbiyos, when cultured on mouse MEF or STO fibroblast feeder layers in presence of hrLW , hrbFGF, hrSCF, and forskolin could survive and give rise to multicellular colonies, resembLing mouse ES-like colonies .

从停经30-70天流产的早期胚胎后肠系膜中或生殖嵴部位分离到的原始生殖细胞,在小鼠的原代MEF或STO成纤维细胞等饲养层细胞上培养,能在hrLIF、hrFGF、hrSCF、forskolin等的共同作用下存活并增殖。

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