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According to these selected interesting rules, we found that some basic information such as survey sites, region, sex, age, smoking status, drinking status, marital status, family month income, occupation, health status, education level, spirit status, physical activity or exercise and family history of gastrointestinal (GI tumors or other GI diseases, some anamneses (for example dyspepsia, gastritis, rheumatic arthritis, chronic faucitis and history of abdominal surgery), and as well some diseases diagnosed by RomeⅡ(such as irritable bowel syndrome, aerophagia and unspecific functional bowel disorder) were associated with GERD symptoms.

根据最终选取的规则,本研究发现调查点、调查区域、性别、年龄、吸烟、饮酒、婚姻状况、家庭月收入、职业、健康状况、文化程度、精神状况、体力活动或体育锻炼、胃肠疾病或肿瘤家族史等被调查者基本信息,部分既往患病史(如消化不良、慢性胃炎、风湿性关节炎、慢性咽喉炎、腹部手术史等),以及肠易激综合征、吞气症、非特异性肠功能紊乱等现患疾病对胃食管反流症状有影响。

Causing expulsion of intestinal worms; anthelmintic.

驱蠕虫药,驱肠虫药一种驱逐肠内蠕虫的药

objectiveto summarize the clinical effect of repairing soft tissue defects in lower part of shank, the foot, with antidromic island flap providing blood supply by suralis nerver.methodsfrom january to december 2006,there were 10 cases repairing soft tissue defects with antidromic island flap providing blood supply by suralis nerver, including 9 males and 1 females.

目的:初步总结腓肠神经营养血管逆行岛状皮瓣在修复小腿中下段及足部创面的临床效果。方法:2006年1月~12月,我科采用腓肠神经营养血管逆行岛状皮瓣共10例,皮瓣最大面积为18cm×13cm,最小为4.5cm×10cm;其中男9例,女1例。

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明,一方面食物进入胃肠道后,激活肠道的内分泌细胞,分泌多种脑肠肽如CCK-8S,这些脑肠肽在血中的水平作为外周信号可通过"肠—脑轴"传入中枢神经系统介导消化和摄食行为的部位,影响摄食中枢神经元的活动调节动物的摄食行为;另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食,如中枢鳃旁体神经元的神经纤维就可延伸到达VMH,并且合成释放内源性的CCK-8S。

Objective:to investigate the effect of damage control surgeryin the theatment ofacute mesenteric ischemia.methods: a retrospective analysis was made on clinical data of eight cases with ami treated by dcs from june 2006 to december 2007.results: operations were immediately performed in all eight patients with ami after final diagnosis,including six cases treated with different limitation of enterotomy; five with temporary abdominal closure and exteriorization of intestine,three with abdominal closure of venous nutrition transfusion container; seven with two-stage operation in different means.five cases survived or improved three died, one of which died two months after discharge due to short bowel syndrome.conclusion:it is very difficult to treat the ami patients owing to the restriction of technology and equipment.

目的:探讨损伤控制外科在治疗急性肠系膜缺血疾病中的应用。方法:对2006年6月~2007年12月应用dcs理念指导救治的8例急性肠系膜缺血疾病的临床资料进行回顾性分析。结果:对8例急性肠系膜血管缺血疾病均于确诊后立即手术探查,其中6例做了不同范围的肠切除;5例做暂时关腹术加肠外置,3例用静脉营养输液袋关腹;7例进行不同方式的二期手术。本组5例痊愈或好转出院;3例死亡,其中1例为短肠综合征出院2个月后死亡。结论:在治疗急性肠系膜血管缺血疾病病人的治疗中,在许多医院因设备和技术等条件的限制,处理十分困难。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例,在入院当天采集深部呼吸道吸引物,用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养,然后提取深部呼吸道吸引物中病原体的DNA,采用荧光定量单PCR的方法检测肺炎支原体,并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因,扩增产物用Luminex100多功能悬浮点阵仪检测。

These results suggest that the activate spleen-energy method can achieve appetitive purpose by not only affecting the gastrointestinal tract function in periphery, but also regulating the process of ingestion in brain. The basic research about ingestion indicate: one hand, after food enters the gastro-intestinal tract, the endocrine cell of the intestinal tract is activated and secrete many kinds of brains intestines peptide, the level of the brain intestines peptide in blood as the periphery signal spreads to the central nervous system regions that control digest and feeding behavior. On the other hand, it has been shown that dorsal parabranchial neurons, containing CCK-8S, extend fibers to the VMH and are involved in the inhibition of feeding .

关于摄食控制的基础研究表明,一方面食物进入胃肠道后,激活肠道的内分泌细胞,分泌多种脑肠肽如CCK-8S,这些脑肠肽在血中的水平作为外周信号可通过&肠—脑轴&传入中枢神经系统介导消化和摄食行为的部位,影响摄食中枢神经元的活动调节动物的摄食行为;另一方面中枢神经元可合成释放内源性的神经肽直接作用于摄食中枢神经元调节摄食,如中枢鳃旁体神经元的神经纤维就可延伸到达VMH,并且合成释放内源性的CCK-8S。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时,本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上,经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后,得到阳性重组质粒pET32a-σC;将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达,经SDS-PAGE和Western-blbtting检测分析,融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应;将融合表达的蛋白纯化后作为包被抗原,建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法,此方法中抗原的最佳包被浓度为5μg/ml、标准阳性血清的最适稀释倍数为1:40倍,用此方法对50份鸭血清样品进行检测,并与琼脂糖凝胶扩散试验检测抗体的法相比较,证明此ELISA方法具有良好的特异性和敏感性,本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

There was 4 amino acids in fat body which were different from midgut.

中肠和脂肪体氨基酸序列有4个氨基酸位点不同,中肠丝氨酸Ser-脂肪体为天冬氨酸N、中肠丙氨酸A-脂肪体缬氨酸V、尤其是脂肪体部位中的血红素保守区由甲硫氨酸M变为缬氨酸V,有可能改变了对底物的催化活性,这在比较的其它几种CYP6B家族的基因中没有发现。

The purposes of this study were to describe the epidemiology of enterovirus infection in Taiwan and to discuss the utility of different statistical methods in analyzing seasonal variation of communicable diseases.

因此本研究的目的是使用官方监测肠病毒的资料描述肠病毒在台湾的发生情况,并采用不同的统计方法,分析肠病毒发生的季节变动趋势。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。