肠

Characterization of the immunoreactiity measured with these assays was performed using gel filtration of human pancreatic juice before and after actiation of trypsinogen with enterokinase.

通过分别在胰蛋白酶被肠激酶激活前后对人胰液的凝胶滤过来定量测定这种免疫反应的特点。

In comparison to the vector containing 4-exa gene without spacer peptide, the expression level of vectors containing glycosylation site sequence and enterokinase recognition site sequence as spacer was increased by 4 and 9 times, respectively.

在EXA的N端增加间隔肽可促进蛋白分泌，与不含间隔肽序列的4拷贝EXA串联表达载体相比，含有糖基化位点和肠激酶识别位点间隔肽的插入，使蛋白表达水平分别提高了4倍和9倍。

The fused protein from pPIC9KBC/KM71 yeast strain had the insecticidal activity and could be digested by enterokinase in vifro.

酵母中表达的融合蛋白具有杀虫活性，并且可以被肠激酶进行体外切割。

After the His·Tag fusion protein was cleaved by the enterokinase, intact biologically active PAT was released from the fusion protein and was purified to homogeneity with Ni2+ affinity chromatography .

用肠激酶切除了融合蛋白的融合部分之后再一次通过金属鏊和层析，经过透析后获得了 PAT 纯品。

The expressed IL-29 fusion protein was purified by Ni-NTA affinity chromatography and the fusion tag was removed from IL-29 fusion protein by cleavage with enterokinase.

纯化后的融合蛋白经肠激酶切割和回收后，所得目的蛋白（IL-29）纯度大于96％，该蛋白N-端序列与理论值一致，其抗病毒活性与IFN-α2b相当。

In the small intestine by enterokinase.

胰蛋白酶原胰朊酶的惰性前体；由胰制造并由肠激酶在小肠内转化为胰朊酶

Methods The cDNA coding sequencing of enterokinase light chain was amplified by RT-PCR from mouse dodecadactylon mucosa and cloned into pET32a expression plasmid. The recombinant enterokinase light chain was expressed in BL21(DE3) and purified with Ni-affinity chromatography.

采用RT-PCR从C57BL/6J小鼠的十二指肠肠系膜黏膜组织中钓取肠激酶轻链的cDNA，将其克隆入pET32a原核表达载体中，并在大肠杆菌BL21(DE3)中进行表达，然后以镍亲和层析法对表达产物进行纯化。

Enterokinase was a serine protein hydroltyic enzyme that could recognize amino acid sequence DDDDK and cleave the peptide bond after "K".

肠激酶(enterokinase， EK)是一种专一识别DDDDK氨基酸序列、并水解K后肽键的丝氨酸蛋白水解酶。

III For constructing the expression vector of a fusion protein and obtain a target protein with full identity on aa sequence of a natural 13- 1,3-1 ,4-glucanase, with the recombined plasmid DNA harbouring the target gene as template, the primers designed with restriction sites for both terminals and enterokinase recognition site, followed by PCR amplification, was induced to the target gene.

为构建融合蛋白表达载体和获得与天然蛋白质序列完全一致的目的蛋白，以含有目的基因的重组质粒DNA为模板，设计引物时加入两端酶切位点及肠激酶裂解位点，通过PCR扩增引入目的基因中，测序结果表明接头和读框正确。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。