肠

The study was aimed to investigate the technological parameters of rhIL-11 preparation from fusion protein by using enterokinase.

本研究探讨利用肠激酶加工融合蛋白的方法制备重组人白细胞介素11(rhIL-11)的工艺条件与参数。

This investigation would be able to lay a foundation for enterokinase activity research and farther application of expression products on a large scale.

该粗酶经脱盐后在适宜的缓冲体系中21℃温育过夜，显示出较高的自催化切割活性，为进一步进行重组牛肠激酶活性的研究及应用奠定了基础。

After fermentation and product purification, we got some purified fusion protein SOD-Thyα1. And the Enterokinase digestion of fusion protein was also studied.

经发酵和纯化得到了融合蛋白SOD-Thyα1的纯品，并对融合蛋白的肠激酶切割特性进行了初步研究。

In conclusion, the preparation method of rhIL-11 from fusion protein by using enterokinase is simple and feasible with good separation, which can meet industrial requirements.

利用肠激酶加工融合蛋白以制备rhIL-11的方法简单可行，分离纯化效果好，能够满足工业生产的需要。

The recombinant mouse enterokinase light chain could be well expressed in Ecoli, but most of them were inclusion.

利用pET32a/BL21(DE3)表达系统成功地在大肠杆菌中表达了重组鼠源性肠激酶轻链，表达量约占大肠杆菌BL21(DE3)总蛋白的30%，但多以包涵体的形式存在。

The inactive precursor of trypsin, produced by the pancreas and converted to trypsin in the small intestine by enterokinase.

胰蛋白酶原胰朊酶的惰性前体；由胰制造并由肠激酶在小肠内转化为胰朊酶

Recombinant hK5 with native N-terminus was generated by enterokinase cleavage and affinity chromatography purification.

经肠激酶切割和纯化，获得了与天然蛋白N-末端相同的hK5重组蛋白。

We also inserted an Enterokinase recognition site between the hSOD gene and the small peptide gene, then we can cleave away the small peptide from fusion protein easily.

在构建时还在小分子多肽与人SOD的连接区插入肠激酶识别位点，这样可以使小分子多肽能从融合蛋白中切割下来。

Having been renaturalized, the fusing protein product is digested with enterokinase, purified by Ni-sepharose and HPLC.

复性、肠激酶酶切、Ni-Sepharose和HPLC纯化后，用质谱测定其分子量并进行活性测定。

The resulting was cleavaged with Enterokinase afte lectrelution and dialysis to carries on the inhibiting test and demonstrated activated.

对目的条带电洗脱、透析回收、肠激酶切割后进行抑菌实验，显示有活性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。