肠

This reserch was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods : everted sac technique, body circulatory perfusion technique and Caco-2 cell method.

本研究首次运用外翻肠囊法、在体肠循环灌流法和Caco-2细胞模型法研究了灯盏花素的肠吸收动力学。

ABSTRACT This research was studied on the Breviscapine intestinal absorption dynamics first-time with 3 methods: everted sac technique, body circulatory perfusion technique and Caco-2 cell method.

本研究首次运用外翻肠囊法、在体肠循环灌流法和Caco-2细胞模型法研究了灯盏花素的肠吸收动力学。

Methods: Everted gut sac was used to compare the absorption of oxymatrine in jejunum, ileum, duodenum and colon.

摘要目的选择大鼠各个肠段进行肠吸收试验，考察氧化苦参碱在大鼠小肠十二指肠、空肠、回肠及结肠部位的吸收情况，确定是否存在药物特异性吸收部位，以此来证明氧化苦参碱制备成缓释制剂的可能性。

Methods After Candida albicans were inoculated into the specific pathogen free mice, the total amount of Candidas in cecum and the amount of which attached to the mucosal membrane were counted at different interent intervals. The lymphocyte proliferation in Peyer's patch and in lamina proper was shown by BrdU incorporation, meanwhile B cells isotype switch in PP was investigated. IgA plasma cells were shown by immunohistochemical staining. Specific IgA antibodies to Candida albicans were measured with ELISA.

采用无特殊病原菌动物经口喂入白念菌，在不同时相处死后，观察肠内白念菌总数及肠粘膜表面白念菌粘附数量；取肠系膜淋巴结做白念菌选择培养，观察移位体内发生率；采用Brdu体内掺入显示局部粘膜淋巴细胞的增殖情况；流式细胞计数潘伊尔氏结细胞表面IgA阳性率；免疫组化染色后计数固有层中IgA浆细胞数量变化；ELISA法测定肠粘液中特异抗白念菌IgA含量。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Result The optimum reaction temperature in stomach, pyloric caecas, foregut, mid-intestine, hindgut and liver were 40-45, 40, 35-40, 40, 40 and 40℃ resp. for protease, 40, 30, 30, 30, 40 and 30℃ for amylase, 40, 40, 35-40, 40, 40 and 25℃ for lipase.

结果］胃、幽门垂、前肠、中肠、后肠、肝胰脏内，蛋白酶最适反应温度分别为40~45、40、35~40、40、40、40℃；淀粉酶的最适反应温度分别为40、30、30、30、40、30℃；脂肪酶最适反应温度分别为40、40、35~40、40、40、25℃。

The results showed that the digestibility and enzymolysis rate were in the order of foregut, midgut, hepatopancreas, hindgut and stomach.

结果显示：离体状态下兰州鲇消化道各部位的消化能力和酶解能力依次为前肠、中肠、肝胰脏、后肠和胃。

Methods BTXA labeled with125Iwas obtained. The radioactivitymeasurementwas taken tomeasure the solubility ofBTXA in gel and BTXA solution. The rightgastrocnemiusmuscles of12 SD ratswere injectedwithBTXA geland the leftgastrocnemiusmuscles injectedwith normal salinewere used as controls. HE staining and 1% gold chloride stainingwere used to study the tissue changes 6 months and 12 months afterBTXA gel injection and transmission electronmicroscopy was used to observe the ultrastructural changes.

将BTXA行示踪标记配制125I-BTXA水凝胶与125I-BTXA水溶液，进行样品放射性强度测定。12只SD大鼠用随机数字表法分为A、B两组，每组6只，大鼠右侧腓肠肌注射BTXA水凝胶，左侧腓肠肌注射等体积生理盐水作对照，于注射后6个月、12个月切取腓肠肌标本，常规病理HE染色、1%氯化金染色，透射电子显微镜观察组织结构变化。

Of or relating to or inside the intestines .

肠的，关于肠的或在肠内。

Gradients of K(superscript +), Na(superscript +) and protein along midgut were determined. The permeability of midgut segments to K(superscript +), Na(superscript +), Ca(superscript 2+), Mg(superscript 2+) and methyl blue was observed.

对中肠细胞进行了组织切片观察；测定K，Na和蛋白质沿中肠的浓度梯度，并观察了中肠对K，Na，Ca(上标 2+)，Mg(上标 2+)和甲基蓝的通透性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。