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The effect of concentrations of Zn, Cu, Pb, Cr, Cd on the viscosity and gel properties of actomyosin and myosin was determined by using texture analyzer after addition of heavy metals.

为了解重金属离子污染对鱼体质地的影响,通过外源添加不同浓度的Zn、Cu、Pb、Cr、Cd重金属离子,采用质构仪测定添加不同浓度金属离子对肌动球蛋白和肌球蛋白黏性和凝胶特性的影响。

Results: MC of tryptase immunoreactivity contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase temporarily was stored in secretory vacuole, and small amounts tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果:类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环,在质膜内层区域形成阻碍类胰蛋白酶释放的屏障;大量的类胰蛋白酶暂存于分泌泡中,少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

Results: MC of tryptase immunoreactivity MC(subscript T contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase was temporarily stored in secretory vacuole, and small amounts of tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果:类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环,在质膜内层区域形成阻碍类胰蛋白酶释放的屏障;大量的类胰蛋白酶暂存于分泌泡中,少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

The changes included that the protrusions on the cell surface diminished, myofilaments and pinosomes in the myoepithelial cells of grade Ⅱ,Ⅲ atypical hyperplasia decreased, and the irregularity of the nuclear morphosis and the increase of nuclear heterochromosome were found.

乳腺不典型增生时肌上皮细胞数目增多,排列紊乱,电镜下可见:①细胞表面的棒状突起消失;②Ⅱ、Ⅲ级不典型增生时胞浆内的肌微丝和吞饮小泡减少;③细胞核增大,不规则,异染色质增多。

PH-20 is a traditional sperm membrane antigen,and it is a glycophosphatidylinositolanchored hyaluronidase.

PH-20(posterior head 20)作为传统的精子膜抗原,是由磷脂酰肌醇(glycophosphatidylinositol,GPI)锚定的膜结合型透明质酸酶,最适PH值呈中性,它分布于精子表面浆膜和顶体内膜,是一种透明质酸酶诱导信号转导的受体和识别透明带的受体。

First, we designed to determine the optimum ultrasound-setting, such as power level and ultrasound frequency, to achieve the optimum number of ruptures in the spinotrapezius muscles by intravital microscopy.

我们设计了应用活体荧光显微镜,测量不同条件超声介导微泡破坏作用后鼠脊斜方肌血管破裂口的数量和荧光脂质体微泡的弥散面积,确定最佳超声发射参数;然后,在慢性鼠脊斜方肌模型上,测量荧光标记蛋白的荧光强度和血管密度,对比两种血管内VEGF基因载体系统经超声介导微泡破坏作用后定向转基因表达的效果。

The results revealed that screwdrivers with triangle shaped handle could help subjects produce the maximum torque. Besides, using screwdrivers with hexagon shaped handle spent the least time, generated the minimum biceps muscle activity, and bad the least subjective fatigue rating. Moreover, wearing an anti-static glove or being barehanded can produced the maximum torque and spent the least time as well. Experiment 2 evaluates the handle diameter of screwdriver (30 mm, 34 mm or 38 mm) and operation plan. The results showed that the torque increased with the increase of the radius, the operation time and the brachioradialis muscle activity also increased with the increase of the radius.

本 研究共分为三次实验,实验一分析握把造型(八角形、锐角六角形、圆角六角形、枪型、圆角三角形)与手套(棉质手套、防静电手套、赤手)的影响,结果显示三角形造型握把可产生最大扭力,六角形造型握把的锁附螺丝时间最短、二头肌肌肉活动度最低、主观整体疲劳评比最低,防静电手套与赤手状态的扭力最大并且锁附螺丝时间最短;实验二比较握把直径(30 mm、34 mm、38 mm)与作业面的影响,结果显示直径越大扭力越大,但直径越小锁附螺丝时间则越短,且肱桡肌肌肉活动度越低。

Methods:Single guinea pig ventricular myocytes were obtained by retrogradely perfusing with collagenase through the aorta.

用主动脉逆向灌流法酶解分离得到单个豚鼠心室肌细胞,在倒置显微镜下,选择质膜完整、横纹清晰的心室肌细胞,利用膜片钳技术经过封接、破膜、电容及串连电阻的补偿形成膜片钳全细胞记录状态。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C,在证实其可以在真核细胞中表达之后,将其用脂质转染剂包裹,形成LipofectAMINE-pcDNAHCV-C脂质混合物,将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌,以空载体pcDNA3做对照,ELISA法检测血清中抗体产生水平。

The rate of enteric transit was measured, and the histological changes in nitriergic nerves in intestinal myenteric plexus of scalded rat were observed as well. The densities of neurons and ganglions with positive nitric oxide synthetase as well as ICC in duodenal myenteric plexus were assessed for both groups.

用酶组织化学方法观察回肠肌间神经丛内氮能神经的组织学改变,并对两组大鼠回肠肌间神经丛内一氧化氮合酶阳性神经元、神经节和Cajal间质细胞的密度进行测定。

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