肌质的

Methods:Via a cannula iserted into adominal aorta, Gey's balanced salt solution containing 0.025% collagenase P was perfused into rat normal pancreas. After digestion with collagenase P and protease, Nycodenz density gradient centrifugation was performed to isolate PSC. PSC was identified by its morphology, cytoplasmic lipid droplets and immunocytochemical staining for desmin, GFAP and α-SMA. Results:The production,viability and purity of isolated PSC were（15.3 ± 4.6）× 103／g body weight,（95.0 ± 3.5）%, and ﹥80％ respectively.

大鼠腹主动脉插管灌注后，经胶原酶消化、Nycodenz密度梯度离心，分离得到PSC；通过观察细胞形态、胞质内脂滴和免疫细胞化学方法检测结蛋白、神经胶质酸纤维蛋白（glial fibrillary acidic protein，GFAP）、α平滑肌肌动素（α-smooth muscle actin，α-SMA）的表达来鉴定PSC。

Atrophy of myocardium, expansion of mitochondria,destruction of myofibril and interstitial fibrosis are the main morphological changes of diabetic cardiomyopathy, and lipid peroxidation and NO may be involved in it.

糖尿病大鼠心肌病变主要为心肌萎缩、线粒体扩张及肌原纤维破坏，间质纤维增生，脂质过氧化作用及NO所致的损伤可能参与其中。

Raffinose, stachyose, and Galpi were found to have good glass forming properties, and their glass transition temperatures were 67.4, 66.4, and 33.40℃, respectively.

棉子糖、水苏糖和甲基肌醇半乳吡喃糖有良好的玻璃质化特性，其玻璃质化转移温度分别为67.4、66.4和33.4℃。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Hematocrit and blood viscosity at shear rate in 150/s, 30/s, 5/s, 1/s, renal fibrosis serum markers, serum creatinine and blood urea nitrogen were detected respectively in sham operation and model group; microcirculation microscope was used to observe the thread of hypoglottis and auricle in rats.

建立单侧输尿管梗阻致大鼠肾间质纤维化模型，观察肾间质纤维化大鼠全血粘度150/s切变率、30/s切变率、5/s切变率、1/s切变率、红细胞压积、舌下及耳廓的脉络变化，同时检测肾纤维化指标、血清肌酐及尿素氮。

Resujts:① The distribution of lesions are mostly symmetrical, extensive, predominant in the middle and lower lung fields, and posterior areas in peripheral;② There are so many HRCT findings of lung, ILD is predominant: intralobular interstitial thickening 46 cases (100%), ground-glass opacity 41 cases (89.13%), peribronchovascular or centrilobular interstitial thickening 40 cases (86.96%), interlobular septal thickening 38 cases (82.61%), irregular linear opacity 37 cases (80.43%), small nodular opacity 34 cases (73.91%), subpleural line 27 cases (58.70%), bmnchiectasis or bronchiolectasis 19 cases (41.30%), patch opacity 18 cases (39.13%), expiratory mosaic sign 15 cases (32.61%), interface sign 14 cases (30.43%), honeycombing 12 cases (26.09%), emphysema or bulla 3 cases, cystic airspace suspected 1 case, and atelectasis suspected 1 case;③ Mediastinum and pleura: multiple small lymphonodi in mediastinum 41 cases (89.13%), pleural thickening or rough 38 cases (82.61%), esophagoectasis 11 cases (23.91%), unilateral little pleural fluid 1 case, and mediastinal emphysema 1 case.

结果：①皮肌炎肺部病变分布呈对称、广泛、偏中下、偏外后的特点；②肺部HRCT表现多样，以肺间质性改变为主：小叶内间质增厚46例(100%)，磨玻璃影41例(89.13%)，支气管血管束增宽或小叶核心增大40例(86.96%)，小叶间隔增厚38例(82.61%)，不规则纤维索条影37例(80.43%)，结节影34例(73.91%)，胸膜下线27例(58.70%)，支气管或细支气管扩张19例(41.30%)，斑片影18例(39.13%)，呼气相马赛克征15例(32.61%)，界面征14例(30.43%)，蜂窝影12例(26.09%)，肺气肿或肺大泡3例，单纯囊状气腔1例，肺不张l例；③纵隔及胸膜：纵隔小淋巴结影41例(89.13%)，胸膜增厚或毛糙38例(82.61%)，食管扩张11例(23.91%)，单侧少量性胸腔积液1例，纵隔气肿1例。

Results The expression of vimentin in interstitial tissue, cytoplasm of interstitial cells, cytoplasm of Sertoli, cytoplasm of myoid cells were positive, not excepting cytoplasm of spermatogoniums.

结果 在染锰大鼠睾丸中除间质，间质细胞、支持细胞，肌样细胞细胞质波形蛋白表达阳性外，也有精原细胞的细胞质VM表达阳性。

Whereas osteoporosis is unassociated with bone pain, osteomalacia has been associated with isolated or generalized bone pain.39,40 The cause is thought to be hydration of the demineralized gelatin matrix beneath the periosteum; the hydrated matrix pushes outward on the periosteum, causing throbbing, aching pain.7 Osteomalacia can often be diagnosed by using moderate force to press the thumb on the sternum or anterior tibia, which can elicit bone pain.7,40 One study showed that 93% of persons 10 to 65 years of age who were admitted to a hospital emergency department with muscle aches and bone pain and who had a wide ariety of diagnoses, including fibromyalgia, chronic fatigue syndrome, and depression, were deficient in itamin D.41

然而，骨质疏松症与骨痛无关联，而软骨病则与局部性或全身性骨痛有关。其原因被认为是骨膜下已去矿质化的胶原基质上发生的水合反应，水合的胶原基质在骨膜上向外扩张，引起阵痛。软骨病可通过以拇指适度挤压胸骨和胫骨前方以引起骨痛感。一项研究显示10岁到65岁中有93%的人向医院急诊室承认有肌肉疼痛和骨痛症状，他们还有一些其他症状包括纤维肌痛、慢性疲劳综合征、抑郁等，该研究显示他们都缺乏维生素D。本人已认领该文第9、10两部分，48小时后若未提交译文，请其他战友自由认领。本人已认领该文11、12部分编译，48小时后若未提交译文，请其他战友自由认领。本人认领第十三部分，48小时内交稿请战友纠错！

The injured gill cells had a different ultrastructure, with endoplasmic reticulum dilated and vesiculated, mitochondrial cristaes partially disintegrated, gill fibre cuticles denatured and dropped. The ultrastructural damages to hepatopancreatic cells were characterized by the decrease of microvilli, swelling and breaking up of mitochondria, expansion of endoplasmic reticulum, disruption and vacuolization of nucleus, tumefaction of nuclear membrane and increase of lipid droplets. As for the toxicological changes of myocardial cells, the mitochondria were swelling, having a lot of vacuolus and disintegrating, while the myofibrils were irregular with endoplasmic reticulum dissolved.

鳃细胞的损害表现为鳃丝水肿，细胞器溶解，角质层变薄，线粒体、内质网的肿胀、解体；肝胰脏细胞的主要损害特征为肝管微绒毛减少，线粒体水肿解体，内质网扩张，细胞核空泡化，核膜水肿和脂肪滴增加；心脏细胞的毒理变化为线粒体内嵴肿胀、瓦解，肌原纤维不规则，内质网溶解。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。