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Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞:弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等;腺上皮下的基底膜区有少量结缔组织存在;扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况;透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞;近基底膜区观察到富含粗面内质网的细胞;基底膜间为薄层疏松结缔组织,内含肌细胞及神经末梢等结构。

FN and LN (the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by typeⅠand Ⅲ collagen ,α-SMA was chosen as a symbol of the activated reposit fat cell. TGF-β〓 was chosen as a symbol of the cellular factor made the reposit fat cell activated. We observed the mechanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN,胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标;用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标;用转化生长因子-β〓作为激活贮脂细胞的细胞因子指标;通过观测上述指标在实验中的变化,来探讨"268方"的作用机理。

FN and LN(the non-collagenous index) out of ECM were measured by immuno-histochemistry mean, and the accumulation of ECM was measured by type I amd III coHagen, a -SMA was chosen as a symbol ef .the activated reposit fat cell.TGF- P1 was chosen as a symbol of th ieccellular factor made the reposit fat cell activated.We observed the mi sclnanism of 286 through the variety of the upper indexes during the experiment.

用免疫组化方法测评肝细胞外基质中的非胶原指标FN、LN,胶原指标Ⅰ、Ⅲ型胶原等在实验过程中的变化作为判定ECM沉积增多的事实指标;用a-平滑肌肌动蛋白作为贮脂细胞被激活的标志物指标;用转化生长因子-β_1(TGF-β_1)作为激活贮脂细胞的细胞因子指标;通过观测上述指标在实验中的变化,来探讨"268方"的作用机理。

Results In dioestrous mice, many iNOS+ cells were found in the stroma of endometrium. There was no obvious differences between the immunized mice and the control mice. In the uterus on 3 day of pregnancy, however, there was obvious difference about the iNOS quantity in uterus between the immunized and the control mice. There was strong positive reaction on the epithelia of endometrium and the endometrial gland in the lamina propria in control mice, while the staining of iNOS in the uterus of SA-30 immunized mice was very weak, only a few of iNOS+ cells were observed in the myometrium.

结果 SA-30免疫后对间情期小鼠子宫内的iNOS没有明显瘙痒影响,无论是免疫组还是对照组,在子宫内膜基质中均有大量阳性偏瘫细胞分布,两者无明显瘙痒差异;在妊娠第3d时,对照组的小鼠子宫中有较多iNOS表达,阳性标记主要集中在内膜上皮及固有膜中的子宫腺上,经SA-30免疫的小鼠子宫中,iNOS表达则显著减少,整个内膜标记极弱,只在子宫肌层内有少量iNOS阳性偏瘫细胞分布。

Results CsA clearly decreased creatinine clearance and increased urinary excretion of N-acetyl-β-D-glucosaminidase in rats as compared with those in the vehicle group. Morphologic changes consisted of vacuolization of tubular cell, expansion of the interstitium and interstitial fibrosis, hyalinization of afferent arterioles. Severity of the injury was related to the dosage of CsA. Striped interstitial fibrosis and arteriolopathy were more severe in the rats treated with CsA and low-salt diet than with CsA and normal diet.

结果 用CsA的大鼠出现尿量增多、内生肌酐清除率下降、尿N-乙酰-β-D-氨基葡萄糖苷酶排泄增多等;病理上表现为不同程度的细胞浊肿、空泡变性及灶性分布的间质纤维化和小动脉病变,随CsA剂量的增加,病变呈加重趋势;结合低钠饮食,CsA慢性肾毒性病变明显加重。

In recent years, ENK-IR neurons in myenteric plexus have been discovered. ENK is one of the important neurotransmitters in ENS. It can reduce the amplitude of cholinergic fEPSP and the release of Ach so as to inhibit contraction of smooth muscles.

近年发现在肠肌间丛神经元中有ENK-IR神经元,脑啡肽是肠神经系统中重要的神经递质之一,可降低胆碱能快速兴奋性突触后电位的幅度,减少乙酰胆碱的释放,抑制平滑肌的收缩。

The mouse was carried out half nephric duct ligation to induce the renal interstitial fibrosis animal model,afterwards,to set up three groups,control group,sham operation group(intragastric administration with normal sodium according to 10ml/kg weight),treatment group...

采用小鼠单侧输尿管结扎法诱导肾间质纤维化的动物模型,用盐酸青藤碱治疗,并设对照组、假手术组(小鼠每日按10m l/kg体重用生理盐水灌胃,直至治疗结束)、治疗组(选用正清风痛宁缓释片按每日30mg/kg体重给小鼠灌胃,连续3周,),检测血尿素氮、血肌酐、血清层粘连蛋白、纤维连接蛋白的含量,免疫组化法测肾脏组织转化因子β1(TNF-β1)的表达并进行肾脏病理学的观察。

Signalling through CXCR4 mediates actin polymerization and pseudopodia formation in cells, and induces chemotactic and migration to target organs. In addition, CXCR4-SDF-1 axis can promote tumor angiogenesis and growth.

CXCR4在肿瘤组织中的表达明显高于正常组织,而肿瘤常见转移部位均为CXCR4的配体基质细胞衍生因子-1(stromal cell derived factor-1,SDF-1)表达丰富的器官,SDF-1与CXCR4结合后可使细胞肌动蛋白丝聚合,形成伪足,从而定向诱导肿瘤细胞迁移到特定的靶器官;此外,CXCR4与其配体SDF-1相互作用可以促进局部新生血管作用,利于肿瘤生长。

Among seven different constructions we examined, a DNA fragment, named pCMV-MB-EGFPITR, containing EGFP reporter gene driven by human cytomegalovirus promoter and medaka β-actin promoter, produced the most intense GFP expression.

在我们试验的七个不同构筑质体中,被命名为pCMV-MB-EGFPITR的DNA片段,含有被人类巨细胞病毒启动子和稻田鱼β型肌动蛋白启动子所驱动的增强型绿萤光蛋白报导基因,能产生最强的绿萤光表现。

Studies in neuropsychopharmacology and molecular neurobiology indicate that neurotransmitters and its receptors play important roles in alcohol abuse and addiction, and post-receptor signal transduction pathways, including cyclic adenosine 3', 5'-monophosphate-protein kinase A, phosphoinositide, Ca(superscript 2+)-calmodulin, phospholipase D and tyrosine kinase Fyn signaling cascade.

神经精神药理学和神经分子生物学研究表明,酒精滥用和成瘾不仅与中枢神经系统的神经递质及其相关的受体有关,而且,受体后的信号转导通路参与了酒精依赖的形成过程。其中包括环磷酸腺苷-蛋白激酶A、磷脂酰肌醇、Ca(上标 2+)-钙调蛋白、磷脂酶D以及酪氨酸激酶Fyn等信号途径。

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