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The tongue was pale, thin fur, pulse empty string, blood routine tests showed: WBC 2.0X109 / L HGB 39g / L PLT 15 X109 / L, after diagnosis, I think that the Department of qi and blood deficiency symptoms, with herbs 30: Bazhen Tonga minus 3 grams ginseng Astragalus 12 grams Atractylodes 12 g Chinese angelica 12 grams TGP 9 grams Chuanxiong 6 grams Rehmannia 15 g Poria 12 g licorice 6 grams jujube plus or minus 15 grams with the disease: a guilty conscience Jingji, Canada Schisandra 6 grams, 30 grams keel in order to Ning Anshen; muscle bleed at the nose, plus 15 grams of Agrimony, Eclipta prostrata 12 grams, the patient, after giving medicine to go back one month after conditioning, review routine blood test showed: WBC 2.8 X109 / L HGB 76g / L PLT 133 X109 / L This firm confidence in the treatment of our patients, followed up by phone after the adjustment necessary prescription, to continue taking the medicine, I issued three months after the review of blood as normal, bone marrow hematopoietic function recovery.

舌质淡,苔薄,脉虚弦,化验血常规示:WBC 2.0X109/L HGB 39g/L PLT 15 X109/L,我诊断后认为系气血两虚症状,具方药30剂:八珍汤加减人参3克黄芪12克白术12克白芍12克当归9克川芎6克熟地15克茯苓12克甘草6克大枣15克随症加减:心虚惊悸,加五味子6克、龙骨30克,以宁心安神;肌衄,加仙鹤草15克、旱莲草12克,病人拿药后回去调理一个月后,复查血常规示:WBC 2.8 X109/L HGB 76g/L PLT 133 X109/L,此坚定患者对我们治疗的信心,经电话随诊后,进行调整必要方剂,继续服用我开具的中药三个月后,复查血象正常,骨髓象造血功能恢复。

Some biochemical properties of cathepsin B, D, E, Hand L, calpain I and calpain II, dipeptidyl peptidase I, II, III and IV, and alanyl-, arginyl-, leucyl, and pyroglutamyl aminopep-tidases were introduced and their roles in meat texture were discussed.

分述了组织蛋白酶B, D, E, H 和L、肌钙蛋白I、II、二肽基肽酶 I, II, III ,IV、丙氨酰、精氨酰、亮氨酰及焦谷氨酰氨基肽酶以及它们在肉的质构中的作用。

AIM: To study intravenous transplantation of human mesenchymal stem cells on the life span and pathological change of SOD1-G93A amyotrophic lateral sclerosis mice.

目的:研究静脉移植人骨髓间质干细胞对肌萎缩侧索硬化症模型小鼠生存期和病理变化的影响。

However, there is merely few clinical and experimental reports about CAM in our coutry, even fewer than alcoholic liver disease and alcoholic myocardiopathy. Lots of researchers have observed that nuclear factor-κB and matrix metalloproteinase 2、9 (MMP-2, MMP-9) play a key role in skeletal muscle weakness and amyotrophy.

有资料表明核因子κB和基质金属蛋白酶(MMP-2、MMP-9)的激活在一些肌病肌萎缩中发挥重要作用,本实验旨研究在慢性酒精性肌病的发病过程中这些因子是否有表达和产生影响。

Resujts:① The distribution of lesions are mostly symmetrical, extensive, predominant in the middle and lower lung fields, and posterior areas in peripheral;② There are so many HRCT findings of lung, ILD is predominant: intralobular interstitial thickening 46 cases (100%), ground-glass opacity 41 cases (89.13%), peribronchovascular or centrilobular interstitial thickening 40 cases (86.96%), interlobular septal thickening 38 cases (82.61%), irregular linear opacity 37 cases (80.43%), small nodular opacity 34 cases (73.91%), subpleural line 27 cases (58.70%), bmnchiectasis or bronchiolectasis 19 cases (41.30%), patch opacity 18 cases (39.13%), expiratory mosaic sign 15 cases (32.61%), interface sign 14 cases (30.43%), honeycombing 12 cases (26.09%), emphysema or bulla 3 cases, cystic airspace suspected 1 case, and atelectasis suspected 1 case;③ Mediastinum and pleura: multiple small lymphonodi in mediastinum 41 cases (89.13%), pleural thickening or rough 38 cases (82.61%), esophagoectasis 11 cases (23.91%), unilateral little pleural fluid 1 case, and mediastinal emphysema 1 case.

结果:①皮肌炎肺部病变分布呈对称、广泛、偏中下、偏外后的特点;②肺部HRCT表现多样,以肺间质性改变为主:小叶内间质增厚46例(100%),磨玻璃影41例(89.13%),支气管血管束增宽或小叶核心增大40例(86.96%),小叶间隔增厚38例(82.61%),不规则纤维索条影37例(80.43%),结节影34例(73.91%),胸膜下线27例(58.70%),支气管或细支气管扩张19例(41.30%),斑片影18例(39.13%),呼气相马赛克征15例(32.61%),界面征14例(30.43%),蜂窝影12例(26.09%),肺气肿或肺大泡3例,单纯囊状气腔1例,肺不张l例;③纵隔及胸膜:纵隔小淋巴结影41例(89.13%),胸膜增厚或毛糙38例(82.61%),食管扩张11例(23.91%),单侧少量性胸腔积液1例,纵隔气肿1例。

Results The expression of vimentin in interstitial tissue, cytoplasm of interstitial cells, cytoplasm of Sertoli, cytoplasm of myoid cells were positive, not excepting cytoplasm of spermatogoniums.

结果 在染锰大鼠睾丸中除间质,间质细胞、支持细胞,肌样细胞细胞质波形蛋白表达阳性外,也有精原细胞的细胞质VM表达阳性。

BACKGROUND: Bone marrow mesenchymal stem cells can differentiate into osteoblasts, chondrocyte, sarcoblasts, adipocyte, neuroepithelial cells, as well as vascular endothelial cells under different conditions.

背景:骨髓间充质干细胞在不同条件下可以分别分化为成骨细胞、软骨细胞、成肌细胞、脂肪细胞、神经胶质细胞、血管内皮细胞等。

Methods: BMSC were separated from bone marrow of rabbits. Three groups were divided as Group A: CPC combined with BMSC (3ml) and bFGF (100μg/ml), Groups B: CPC with BMSC (3 ml), and Groups C: CPC. Each group was implanted into the sacrospinalis muscle of rabbits after cultured for 24 h.

取兔骨髓,分离骨髓基质细胞,选取松质骨粒型自骨化磷酸钙置於板孔内, A 组复合 BMSC (3ml)和bFGF(100μg/ml);B组仅复合BMSC(3ml);C组仅为CPC;置於培养箱中,培养24h后植入兔骶棘肌内。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示:睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内,1周龄睾丸组织免疫阳性反应主要位于支持细胞,精原细胞也有着色;3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性;5周后的睾丸组织内神经生长因子呈低水平表达,主要表达于间质细胞和生精细胞内。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C,在证实其可以在真核细胞中表达之后,将其用脂质转染剂包裹,形成LipofectAMINE-pcDNAHCV-C脂质混合物,将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌,以空载体pcDNA3做对照,ELISA法检测血清中抗体产生水平。

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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