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In order to evaluate the effects of calcium ion on calpalns mRNA expression and proteolysis activity,and muscle protein degradation,L6 myoblast and beef fascicle were used in vivo and in vitro samples respectively in this study.

本实验分别以牛肉肌束和分化为肌细胞的L6成肌细胞为研究对象,研究钙离子对离体肌束中钙激活蛋白酶活性、蛋白分解以及活体肌肉中钙激活蛋白酶mRNA水平表达的影响。

The mean proportional volumes of mucous acini were more than those of serous acini except salivary glands of rodents.Conclusion: The mean proportional volume of MeCs in salivary glands associates with animal species and secretion of salivary glands.

HHF 35是近几年开始使用的识别特异性肌动蛋白(muscle-actin-specific)的单克隆抗体,作者使用它来区别肌上皮细胞和腺泡,希望通过定量测定比较肌上皮细胞的平均体积比,以了解不同动物涎腺中肌上皮细胞的发育是否存在物种间的差异及与分泌功能的关系。

This paper studies the changes of the sarcoplasmic and myofibrillar proteins in Semimembranosus and Biceps muscles during the processing of Jin-hua ham .

以金华火腿为原料,主要从原料、腌制期、发酵初期、发酵中期、发酵末期五个阶段取样,对金华火腿在加工过程中半膜肌和股二头肌的肌浆蛋白和肌原纤维蛋白的降解情况进行了研究。

α-actin mRNA level was indeed increased by in tensive exercise and this effect bear a time-preference.

训练的确提高了大鼠骨骼肌α-肌动蛋白的mRNA水平,且运动恢复过程中骨骼肌α-肌动蛋白基因的表达具有时效性。

The content of the myoglobin of the Plateau Zokor was measurated by spectrometer for studying the changes in different seasons.

为了研究高原鼢鼠肌红蛋白在不同季节的变化,用分光光度法测定了肌红蛋白含量。

The results showed that the content of myoglobin in cardiac and skeleton of Plateau Zokor was 1001.25±103.25,726.50±54.70,767.33±88.73 and 781.85±100.98,672.15±108.88,676.80±77.31 in spring, summer and autumn.

结果显示:在春季、夏季和秋季,高原鼢鼠心肌肌红蛋白含量分别为1001.25±103.25,726.50±54.70,767.33±88.73;骨骼肌肌红蛋白含量分别为781.85±100.98,672.15±108.88,676.80±77.31。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长,呈成纤维细胞样形态,经5一aza诱导后细胞体积增大,变为球状或者长杆状,4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性,10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高,但20umol·L一'5一aza组约50%细胞死亡。

Using semi-quantitative PCR within the exponential phase, with beta-actin as an external control, the constitutive transcriptional expression of genes of phase I (CYP1A), phase II xenobiotic metabolizing enzymes, HSP70 and ATP1A1 were also investigated.

应用半定量RT-PCR的方法,以β-肌动蛋白作为外参照,在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA的比值,确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。

The findings indicated that chicken leptin protein could markedly increase abdominal fat deposition in yellow-feathered chicken, did not have significant effect on weight or protein content of leg and breast muscle, fat and water content nor on serum T3 and T4 indices.

本试验首次用主动免疫的方式来检测重组鸡leptin蛋白的生物学活性,结果表明leptin蛋白在黄羽肉鸡体内能明显的增加脂肪沉积,对胸腿肌重量及胸腿肌中蛋白质、脂肪和水分的含量没有明显影响,对血清中T3、T4指标没有影响,对料肉比、期末体重和体增重等生产性能指标的改善有阶段性的作用。

Rabbitfish were administered respectively to injections of DA (2μg/g wet weight) or PBS as controls. After 24 h, rabbitfish were killed and hepatic RNA was isolated.

我们用半定量PCR的方法,并用β-肌动蛋白作为外参照,在其指数期增长的范围内得到CYP1A,GSTA,GSTR,HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA的比值。

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