肌蛋白

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

Results ① The glucose, natrium and chlorine in human vitreous humor decreased, while the urea, creatinine, uric acid, potassium, calcium, magnesium, phosphorus, and micro-protein increased after death.

结果①玻璃体液中葡萄糖、钠、氯随死后时间的延长而逐渐下降；尿素、肌酐、尿酸、钾、钙、镁、无机磷、微量蛋白随死后时间的延长而逐渐升高。

We report the case of a congenital myasthenic syndrome due to a mutation in AGRN, the gene encoding agrin, an extracellular matrix molecule released by the nerve and critical for formation of the neuromuscular junction.

我们报告了一例由于AGRN集聚蛋白编码基因）突变导致的先天性肌无力综合征，AGRN是一种由神经释放的细胞外基质分子并对神经肌肉接头的形成起重要作用。

Preliminary Identification of A Single Dominant Gene Conferring Resistance to Leafminer in Lycopersicon hirsutum Dunal;2. Biological characteristics of adult Opius caricivorae Fischer, a parasitoid of Liriomyza sativae Blandchard ;3. Opius caricivorae Fischer (Hymenoptera: Braconidae) is an important endoparasitoid of the vegetable leafminer, Liriomyza sativae Blandchard (Diptera: Agromyzidae) in China.

本文以检疫性害虫—美洲斑潜蝇 Liriomyza sativae Blandchard及其优势种天敌—黄腹潜蝇茧蜂opius caricivorae Fischer这一寄生体系为研究对象，对黄腹潜蝇茧蜂的毒液器官形态和超微结构、类病毒颗粒存在与否及其分布、毒液的蛋白组分及其对寄主的生理影响等进行了初步的研究，取得以下结果：(1)位于雌蜂腹部近背面的黄腹潜蝇茧蜂的毒液器官属于类型Ⅰ，由一个土黄色、锥状的毒囊、7个透明的椭圆形的毒腺和一个导管构成的，其中毒囊由发达的纵肌环绕。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Objective To study the changes of expression of α-SM-actin in some cerebral arterioles with different severities of Aβ deposit in the cases of cerebral amyloid angiopathy.

目的 脑淀粉样血管病患者不同病变程度脑小动脉中α-平滑肌肌动蛋白表迭变化的对比。

Methods: Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats. The rats were divided into two groups (oestrus group and anoestrus group). The expression of α-SMA and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.

分别取动情期和非动情期大鼠卵巢，运用免疫组化方法检测肌成纤维细胞的特征性标记和波形蛋白分别在这两组中各级卵泡卵泡膜细胞上的表达情况，并进行图像分析。

Methods The level of serum and urinary tissue-type plasminogen activator and plasminogen activator inhibitor-1 (PAT-1) were measured in 38 patients with chronic glomerulonephritis, 28 patients with nephropathy syndrome, 36 patients with chronic renal failure and 20 healthy subjects by means of enzyme-linked immunosorbent assary. The relationship among urinary t-PA, PAI-l and serum t-PA, PAT-1, urinary protein, serum creatinine were also observed. Results Levels of serum t-PA, PAI-1 in chronic kidney diseases increased remarkably compared with control group.

选择38例慢性肾小球肾炎，28例肾病综合征，36例非透析治疗的慢性肾功能不全和20例正常对照作为研究对象，应用ELISA法检测血清和尿液中组织型纤溶酶原激活剂和纤溶酶原激活物抑制剂－1(PAI-1)的浓度，同时分析尿中t-PA和PAI-1的水平与血t-PA、PAI-1、血肌酐和24h尿蛋白总量之间相关性。

Methods］ Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法］ 将SD大鼠随机分成正常对照组、单纯缺血组、再灌注2 h组、4 h组、12 h组、24 h组，制备双侧肾蒂夹闭模型后，腹主动脉取血测血肌酐、尿素氮，行肾脏组织病理学检查，免疫组化法检测TGFβ1 蛋白，RT-PCR法检测TGFβ1 mRNA。

Potassium, natrium determines it is good that use ion chooses electrode indirect method it is better also with indirect method to determine at calcium of direct way;, it is next occasionally phosphor of; of law of azotic arsenic Ⅲ determines use phosphor molybdic acid is ultraviolet law outcome is better it is better with result of law of personal candy kinase that; blood sugar determines; urea determines use Niao enzymatic ultraviolet anhydride of better; flesh uses rate law result enzymatic law outcome is better; albumin determines, it is vanadium acid next alkalescent phosphoric acid of oxidation law; is enzymatic determine outcome of fluid of use AMP amortize is better; amylase determines law of thing of enzymatic standard background and iodic colorimetric law coefficient of variation all bigger, former a bit small, latter of percent of pass is tallish, each lab can try to choose according to his condition.

结论钾、钠测定使用离子选择电极间接法好于直接法；钙测定用间接法也较好，其次是偶氮砷Ⅲ法；磷测定使用磷钼酸紫外法结果较好；血糖测定用己糖激酶法结果较好；尿素测定用脲酶紫外速率法结果较好；肌酐用酶法结果较好；白蛋白测定用溴甲酚绿法较好；胆红素测定用酶法结果好，其次是钒酸氧化法；碱性磷酸酶测定使用AMP缓冲液结果较好；淀粉酶测定酶法底物法和碘比色法变异系数均较大，前者稍小，合格率后者稍高，各实验室可根据自己的条件加以选用。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。