肌蛋白

The result indicates the t-BHP or diamide both cause the mass loss about the membrane protein thiol in hepatocytes, the fast oxidization about glutathione in hepatocytes, the leakage of lactate dehydrodenase, but the level of t-BHP is larger than of diamide. In the aspect of lipid peroxidation, only the t-BHP produces apparent change. As to actin and tubulin, they both find fall-off and present time-dependency effects. The result in Confocal fluorescent microscope also show these two cytoskeletal proteins are related to cell blebbing, but they don''t play the same role during the porcess. Tubulin may be more important than actin. If the study predispose dithiothreitol before adding t-BHP or diamide, then the damage by the diamide is repressed, but just partly protection for the damage by t-BHP.

结果显示t-BHP或diamide均可造成细胞膜蛋白硫醇大量流失与胞内GSH快速氧化，亦显著导致乳酸去氢□渗漏，但t-BHP诱发的渗漏程度大於diamide；在脂质过氧化方面，则仅t-BHP处理组产生明显的脂质氧化情形；至於肌动蛋白与管蛋白单体均可见减少且呈现时间依赖效应；在雷射共轭焦萤光显微镜观察中，亦显现这两种架构蛋白与细胞膜起泡有关，但两者在小泡生成的过程中扮演的角色并不完成相同，可能管蛋白较肌动蛋白重要；如果细胞在t-BHP或diamide加入前预先以dithiothreitol处理，则diamide 所造成的伤害显著的受到抑制，但DTT对t-BHP所诱发的伤害则仅有部分保护效果。

Results These two patients were confirmed to suffer from DMD. They were characterized by typical features of DMD including typical clinical manifestations, increased serum enzymes, EMG presenting myogenic impairment, HE staining presentation belonging to DMD, negative dystrophin in brother, and inconstantly positive on the sarcolemma of sister. Furthermore, no deletion or duplication was found in the 1-79 exons of dystrophin gene. The suffering brother and sister carried the same maternal X chromosome.

结果 兄妹二人符合DMD诊断，具有典型的DMD临床表现，肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、羟丁酸脱氢酶和谷草转氨酶的水平均显著高于正常值，肌电图呈肌源性损害，肌肉HE染色符合DMD，男患者的抗肌萎缩蛋白表达阴性，女患者的少量肌纤维仍可见不连续膜阳性，两患者抗肌萎缩蛋白基因的1~79号外显子未见缺失和重复突变，女患者与男患者携带相同的母源性X染色体。

Transcription, western, and immunohistochemical analysis showed increased levels of dystrophin transcript and protein, and correct localization at the sarcolemma.

用免疫组织化学分析用纳米作为载体进行寡聚核苷酸递送的实验鼠抗肌萎缩蛋白基因RNA和抗肌萎缩蛋白的水平得到提高，而且恢复了抗肌萎缩蛋白在肌肉细胞膜上的正确位置。

Titin is recently known as the largest protein which exists in the stiated muscle sarcomere and is dynamic both in biomechanics properties and biochemical functions. Four possible disease-associated mutations located in three exons(3,14,49) of titin gene have been identified in Japanese DCM patients in 2002. But we did nothing about the creative titin and DCM.

目的：肌联蛋白是由单基因编码的最大蛋白，普遍存在于心肌和骨骼肌，被称为第三肌丝，具有复杂的生物力学性质和生物化学功能。2002年日本研究人员报道了肌联蛋白基因第3、14、49外显子的4个基因突变可能与扩张性心肌病发病相关，而我国没有相关的研究报道。

Fragments from the 239th to the 944th nucleotide of the coding regions of all actin mRNAs were amplified with two actin specific primers. Fragments from the 239th nucleotide of the coding region to the end of actin mRNAs were also amplified The electrophoresis results were compared between the macropterous and brachypterous brown planthoppers.

利用两条肌动蛋白特异性引物，扩增出了肌动蛋白基因mRNA的编码区片段，另外，将肌动蛋白特异性引物作为上游引物，将与3'锚定反转录引物部分序列相同的引物作为下游引物，扩增出了从肌动蛋白基因mRNA的编码区第239号碱基到Poly尾的这段cDNA，并在翅型之间作了比较。

It was found from the structure-electrochemical studies of myoglobin that (1) electroreduction induces the T→R configuration transition with peak shift in CD spectra and without umbrella conversed effect, which proved that umbrella conversed effect is mainly the cooperation of four subunits in hemoglobin;(2) the mechanism of EC with adsorption of reduction product and parameters have also been obtained.

肌红蛋白的结构-电化学研究中发现：（1）电化学还原诱导了肌红蛋白的T→R构型转化，伴随着CD谱的峰位移，但没有象血红蛋白那样明显伞翻转效应，表明伞翻转效应是多个亚基共同作用的结果；（2）获得肌红蛋白电化学还原的EC机理，并伴随产物吸附的自阻碍过程及其参数。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Genome, and its expression products are located in the flagellar, cytoplasm and fertilization tubule. Cr. actin is partially purified from Cr. cells by the method of assembly-disassembly-circle of actin.

我们利用肌动蛋白本身所具有的聚合和解聚的性质，从衣藻细胞中部分纯化了衣藻肌动蛋白，纯化的肌动蛋白能和抗兔α-平滑肌肌动蛋白的单克隆抗体发生专一性很强的免疫交叉反应。

Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化；RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化；抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达，包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases， ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor， Rho-GDI alpha)，研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

When stained with TRICT-conjugated phalloidin, both the nuclei and nuclear matrix specimens were found to give off specific, red fluorescent signals which represent the location of F-actin, and the pattern of TRITC signals distribution in the nuclei and nuclear matrix showed similarity with that of FITC signals.

在荧光显微镜下观察到：代表肌动蛋白的特意性荧光分布在细胞核和细胞骨架中，说明肌动蛋白是细胞核和核骨架的固有成分；代表F-肌动蛋白的特异性荧光存在于细胞核和核骨架中，说明细胞核和核骨架含有F-肌动蛋白。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。