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肌蛋白

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ADF/Cofilins Proteinic Family Constellation in Cytoskeletal Protein: How Does Depolymerization Occur through Combination of ADF/Cofilins with Actin?

CEPS 思博网--文章书目;-1页数:5 需求点数:20 电子全文:请登入。篇名细胞骨架蛋白中ADF/cofilin蛋白家系的作用:如何与肌动蛋白结合及发挥解聚作用?

The PrP of bastard halibut consists of 472 amino acid residues, weighing about 49.6kD. The deduced amino acid sequence is 71.9% similar to that of Japanese pufferfish and 66.4% similar to that of Atlantic salmon, and bears the structural signatures of PrPs including a signal sequence, tandem repeats, a hydrophobic region, two glycosylation sites, a disulphide bridge and a glycosyl phosphatidylinositol anchor site.

推测的牙鲆蛋白质感染因子蛋白由472个氨基酸组成,分子量约49.6kD,具有信号肽、短肽顺接重复、疏水区、糖基化位点、二硫键、糖基缩醛磷肌醇锚定位点等蛋白质感染因子蛋白特征结构域,与红鳍东方鲀和大西洋鲑蛋白质感染因子蛋白的相似性分别为71.9%和66.4%。

The purpose of this study was to examine the cellular surface blebs of oxidative damage. The important is cytoskeletal proteins-actin and tubulin acts in cellular surface blebs.

本实验主要目的在探讨氧化伤害与小泡生成的关系,尤其著重在细胞架构蛋白-肌动蛋白与管蛋白两者在小泡生成时扮演的角色。

Incubated with cytochalisin B for 4 hours, F-actin bunddles disrupted, confluent ECs monolayer has a complete detachment from the coverslips. So, there is a suggestion that the F-actin in ECs is essential for endothelial adherence under shear stress.

Northcrn杂交结果表明外源性纤维连接蛋白有促进EC肌动蛋白表达的作用,结合F-actin染色及FN受体染色结果,本文认为用纤维连接蛋白作为细胞种植的促粘附物质有助于内皮细胞粘附结构的形成。

Recent studies showed that Rho GTPase played a key role in the reorganization of cytoskeleton and in Swiss 3T3 cells, Cdc42, Rac1 and RhoA, three members of Rho superfamily of small GTPase, regulated the polymerization of actin to produce filopodia , lamellipodia, stress fiber and focal adhension, respectively.

近年来的研究表明,Rho家族小G蛋白是细胞骨架肌动蛋白的重要调节因子,目前众多的研究集中在Rho小G蛋白家族成员中的Cdc42、Rac1和RhoA,其中Cdc42促进丝状伪足的形成,Rac1调节板装伪足的生成和膜皱缩,RhoA则促进粘着斑连接和张力纤维的装配。

The gene-expressionprofile might help to further understand the molecular basis of tube worm physiology.It will also lay a good foundation for functional studies on the adaptation to extremeenvironments and help to make use of them. MicroRNAsare a class of small noncoding RNA that are 20-22nucleotidesand function as regulators of gene expression.

其中,几丁质结合蛋白最具多样性,不仅几丁质酶水解区和几丁质结合区相互独立,而且几丁质结合区也显示丰富的多样性,由不同的几丁质结合区共组成了23种不同的几丁质结合蛋白;EST测序分析中还发现两个溶菌酶基因带有双功能区,可能具有双重功能;此外,肌红蛋白基因也显示了序列的多样性。

METHODS Using heparin sepharose Cl-6B chromatography,the osteoinduction protein in reconstituted bone xenograftwas pu-rified and fractions obtained from purification were bioassayed for bone-induction activity by implantation into the thigh muscles of mouse.

采用肝素亲和层析技术纯化RBX中诱骨活性蛋白即骨形态发生蛋白,利用小鼠肌袋实验检测其骨诱导活性,再用聚丙烯酰胺电泳测量RBX中诱骨活性蛋白纯化后的相对分子质量。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation,single fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白,并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性;以提取的蛋白主动免疫Lewis大鼠,每天观察其临床表现,并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

Methods Lewis rats were immunized with the protein extracted from electric organ of Narcine timilei, the clinical manifestations were observed every day, and repetitive nerve stimulation, ingle fiber electromyography and the levels of acetylcholine receptor antibody were detected on the 49th days post immunization. The content of the protein extracted from electric organ of Narcine timile was 35.4 mg/mL, and the protein could respond with AChRAb from the serum of seropositive myasthenia gravis patient.

参照徐氏法从丁氏双鳍电鳐的电器官提取AChR蛋白,并采用Folin-酚试剂法及酶联免疫吸附法检测提取蛋白的含量及活性;以提取的蛋白主动免疫Lewis大鼠,每天观察其临床表现,并于免疫后7周进行低频重复电刺激、单纤维肌电图及血清中AChRAb水平的检测。

The content of Gq protein was measured in the liver tissue by Western blot.

在G蛋白家族中,参与肌醇磷脂信息传递调节的特异性G蛋白是Gq蛋白[1]。

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