肌蛋白

Of these patients, 67,924 had a creatinine level of less than 2.0 mg per deciliter. Cardiac troponin I was measured in 61,379 patients, and cardiac troponin T in 7880 patients (both proteins were measured in 1335 patients). Overall, 4240 patients (6.2%) were positive for troponin.

在这些患者中67924例患者肌酐小于2.0mg/L.61379例患者接受了心肌肌钙蛋白I检查，7880例患者接受心肌肌钙蛋白T检查（1335例患者接受了两种蛋白检查）。4240例（6.2%）患者肌钙蛋白阳性。

Methods:The rats with sepsis was established by administering endotoxin into the peritoneal cavity.The rats were randomly divided into 4 groups as follows:2,6,12 and 24 hours after administration of endotoxin,each group included normal control.We use the in vitro muscle incubation system with sufficient oxygen supply as well as amino acid automatic analyzer for detection of the proteolytic rate of extensor digitorium longus and soleusmuscle.

大鼠腹腔注射内毒素制成脓毒症模型，随机分成内毒素攻击后2、6、12和24小时组，每组设正常对照；利用骨骼肌充分氧供离体孵育系统，采用氨基酸全谱分析仪测定脓毒症大鼠伸趾长肌和比目鱼肌蛋白降解率。

We use the in vitro muscle incubation system with sufficient oxygen supply as well as amino acid automatic analyzer for detection of the proteolytic rate of extensor digitorium longus and soleusmuscle.

大鼠腹腔注射内毒素制成脓毒症模型，随机分成内毒素攻击后2、6、12和24小时组，每组设正常对照；利用骨骼肌充分氧供离体孵育系统，采用氨基酸全谱分析仪测定脓毒症大鼠伸趾长肌和比目鱼肌蛋白降解率。

From the femur bone density analysis of protein expression, we can see that light chain lactoferrin, annexin decreased and the expression of enolase, ATP synthase, acetyl-coenzyme A reductase, the expression of troponin, may be related to ovariectomized femoral bone density in osteoporosis-related happened; strong bone of the femoral osteoporosis Po intervention effect may be related to reduced enolase, ATP synthase, troponin, CK-MB, acetyl phosphoglycerate enzyme, the expression of myosin, and the increase in lactoferrin light chain, pyruvate kinase isoenzyme, protein expression crown.

从股骨密质骨蛋白质组表达分析可知，乳铁蛋白轻链、膜联蛋白A3的表达下降及烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白的表达增强，可能与去卵巢后股骨密质骨骨质疏松的发生相关；强骨宝对股骨骨质疏松的干预效应可能与下调烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白的表达，和上调乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白的表达有关。

Cancellous bone from the lumbar proteomic expression analysis show that actin, keratin, enolase, ATP synthase, the expression of myosin may be related to ovarian lumbar cancellous bone after osteoporosis-related happened; Qiang Gu Bao Po on the lumbar spine osteoporosis intervention effect may be related to reduced carbonic anhydrase, actin, crystal protein, 3- phosphoglycerate dehydrogenase, serum albumin, ATP synthase, myosin, the enolase expression.

从腰椎松质骨蛋白质组表达分析可知，肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白的表达增强可能与去卵巢后腰椎松质骨骨质疏松的发生相关；强骨宝对腰椎骨质疏松的干预效应可能与下调碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶的表达有关。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

The predicted amino acid sequence of the gene show a rather high homology(84.47% amino acid identities)with Haemaphysalis longicornis(H.longicornis) TnI (GenBank,GI:14041807).Furthermore, the actin-binding domains of troponin I in predicted amino acid sequence, just the same as H. longicornis ,at the amino acid residues 147-167, suggesting the gene is K haemaphysaloides TnI gene.

经同源性比较，该基因预测的氨基酸序列与长角血蜱肌钙蛋白Ⅰ(GenBank，GI：14041807)有很高的同源性(同源性为84.47%)，肌动蛋白结合位点位于147-167氨基酸处，且与长角血蜱肌钙蛋白Ⅰ肌动蛋白结合位点完全相同，表明该基因是镰形扇头蜱肌钙蛋白Ⅰ基因。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

In conclusion, continuous exposure to tetracaine at small concentrations delayed neurite growth, reduced the number of filopodia, and decreased actin content.

结论，连续暴露在低浓度地卡因可延缓神经突生长，降低丝伪足的数量和减少神经突肌蛋白数量。

The quantity of actin in cell bodies increased, contrary to the effect on neurites and growth cones, where actin decreased 48 h after the application of 5, 10, and 20 microM of tetracaine.

神经元细胞体内肌蛋白数量增，其与对神经突和生长锥的影响相反——分别给予5、10、20uM 地卡因后48h 其肌蛋白数量减少。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。