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Results These two patients were confirmed to suffer from DMD. They were characterized by typical features of DMD including typical clinical manifestations, increased serum enzymes, EMG presenting myogenic impairment, HE staining presentation belonging to DMD, negative dystrophin in brother, and inconstantly positive on the sarcolemma of sister. Furthermore, no deletion or duplication was found in the 1-79 exons of dystrophin gene. The suffering brother and sister carried the same maternal X chromosome.

结果 兄妹二人符合DMD诊断,具有典型的DMD临床表现,肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、羟丁酸脱氢酶和谷草转氨酶的水平均显著高于正常值,肌电图呈肌源性损害,肌肉HE染色符合DMD,男患者的抗肌萎缩蛋白表达阴性,女患者的少量肌纤维仍可见不连续膜阳性,两患者抗肌萎缩蛋白基因的1~79号外显子未见缺失和重复突变,女患者与男患者携带相同的母源性X染色体。

The main components were a lateral expansion to the oblique popliteal ligament; a direct arm, which attached to the tibia; and an anterior arm. The oblique popliteal ligament, the largest posterior knee structure, formed a broad fascial sheath over the posterior aspect of the knee and measured 48.0 mm in length and 9.5 mm wide at its medial origin and 16.4 mm wide at its lateral attachment. It had two lateral attachments, one to the meniscofemoral portion of the posterolateral joint capsule and one to the tibia, along the lateral border of the posterior cruciate ligament facet. The semimembranosus also had a distal tibial expansion, which formed a posterior fascial layer over the popliteus muscle.

主要的部分是一个可以侧方扩展的腘斜韧带,最大的膝关节后方的组织结构,形成了一个较大的筋膜外皮位于膝关节的后方,测量结果显示长度为48mm,边缘宽度为9.5mm,侧方附件的宽度为16.4mm,它具有两个方面的附件,一个位于半月板股骨间部分的后外侧关节囊和一个在胫骨端的后叉韧带边缘,半膜肌同时也存在一个远处的胫骨扩展,形成了一个后方的筋膜附着腘肌。

According to a completely randomized block design, 54 pigs (Larger White×Landrace) with an average weight of 53kg were assigned to three treatments. The diets contained the same amino acid pattern (TDLys : TDMet : TDThr : TDTrp=100 : 34.6 : 71.7 : 19.2) and digestibile energy levelDE 3200 kcalkg^(-1 with different crude protein levels (CP 10%, 18% and 26%, respectively). The trial lasted for 67 days before slaughter, and longissimus dorsi, psoas major, semaitendinosus, semimembranosus, arm triceps, glutaeus medius, the area, diameter and density of the muscle fibers were measured.

采用完全随机区组设计,54头53kg大×长生长猪随机分为3个处理(每处理3个重复),饲喂等氨基酸(TDLys:TDMet:TDThr:TDTrp=100:34.6:71.7:19.2)、等消化能DE 3200kcalkg^(-1和不同蛋白水平(CP 10%,18%和26%)的3种饲粮,饲喂期67d之后屠宰采样,测背最长肌、腰大肌、半腱肌、半膜肌、臂三头肌、臀中肌的肌纤维面积、直径和密度。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来,在基金委的支持下,我们以蛋白膜伏安法这一新技术作为主要的研究手段,并结合其他生物、化学技术,研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为,结合各种光谱技术,对蛋白质在膜中的构象进行表征,探索其电子传递机理。

In cadavers of fetus(gestational age>28 wks), infant and adult, the PEM fibers(which ran 2 to 4 cm cranially)were attached to the esophagus over its left and anterior aspect above the diaphragm.

膈食管膜分上、下两层,分别起于膈上、下筋膜,上层向上,下层分层为上、下两叶后向上、下止于食管外膜,部分纤维插入纵肌束或环肌束间。

In detrusor-urethral dyssynergia,the membranous urethral pressure elevated on voiding.When there was detrusor-external sphincter dyssynergia,it was further complicated by electric overactivity.In detrusor-bladder neck dyssynergia,the bladder neck pressure sloped down at voiding.

2逼尿肌尿道协同失调的病人,排尿时膜部尿道压升高,其中逼尿肌外括约肌协同失调时合并有肌电活动明显增加,逼尿肌膀胱颈协同失调肌电活动正常,排尿期尿道测压膀胱颈处压力呈斜坡样下降。

Results The supra-orbital nerve passes through the deep fascia of frontal muscle and epicranial aponurosis with an 52.8±7.4° angle before it enters the frontalis. Its diameter is 1.4±0.3 mm.

结果 眶上神经出眶上孔后,以 52.8±7.4°角向外上经额肌筋膜附着处入帽状腱膜下隙,达发际附近穿帽状腱膜和额肌至皮下。

Experiments were carried out on day 0,3,5,7,14 and 21 including the study of the perimysial thickness,fiber diameter,myofibrillar fragmentation index,myofibrils ultrastructure ,and SDS-PAGE electrophoresis.

研究了宰后成熟处理0、3、5、7、14和21 d的花。马杂交F1鹿背最长肌、半腱肌、冈上肌和腰大肌的肌束膜厚度、肌纤维直径、肌原纤维小片化指数、肌原纤维超微结构和肌肉蛋白降解的变化。

Thirty patients who had definite chronic lumboscaral compartment syndrome without other lumbal diseases were choosed respectively to test muscle force of lumbar and abdomen, intra-sacrospinal muscle pressure, blood routine, ESR, Ck, CK-MM LDH and LDHs. All patients received decompressive operation. Skeletal muscle specimens taken from sacrospinal muscle in each operation were possessed for bistological and ultrastrucluml observation. All of enzyme tests were normal.

方法]选取明确诊断为腰骶部慢性骨筋膜间隔综合征且未合并其他腰部疾病的患者30例,分别行腰腹肌肌力测定,竖脊肌内压测定,血常规,血沉,肌酸激酶及同工酶,乳酸脱氢酶及同工酶(LDH5)测定,采用骨筋膜间隔切开减压手术治疗。

Before transecting inject 1ml Sodium Chloride in the epineurium. Annular tensile the proximal of epineurium with microscopic forceps annular shear the epineurium 7mm before broken ends with microscopic shear and stretch the epineurium until the two ends distance is 5mm.Use 8-0 suture line to bridge the nerve gap and make a 5 mm nerve tube at the proximal nerve end. Interrupted suture the epineurium tube and the distal nerve end make a 5 mm nerve mamuro for the injury nerve.Results: Postperative observation lasted for 6 weeks regeneration nerve through the 5 mm nerve mamuro.

在大鼠的坐骨神经距股方肌下缘15mm处,用1ml注射器向神经外膜下注入1ml生理盐水,然后于此处切断坐骨神经,用显微镊环形拉伸近端神经外膜,在距断端约7mm处用显微剪环形剪开神经外膜,并继续拉伸外膜使外膜两断端间距约5mm停止,用8-0的无损伤缝线桥接神经外膜两端,在神经近端形成约5mm的神经外膜管,将神经外膜管与神经远端间断缝合,使断伤的神经束间留有5mm的小间室。

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