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Three methods, which were SDH activity, specific fluorescent probe(Rh123) and mitochondrial 16S rDNA gene amplification, were used to detect sperm mitochondria of Eriocheir sinensis, and three tissues——muscle, branchia and testis, were investigated as control.

以中华绒螫蟹肌肉、鳃和精巢为对照,从线粒体标志酶SDH活性、线粒体特异性荧光探针(Rh123)以及线粒体16S rDNA PCR扩增3个途径,探讨了该蟹精子线粒体的存在状况。

Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物,利用PCR扩增方法,以beta-actin做内参,检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况,发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致,表明各模板DNA量基本一致;而在同一DNA浓度下,16S rDNA的扩增片段长度虽一致,但其产物量存在明显差异,精子16s rDNA产物量显著低于其他3种组织,其条带宽度和亮度很弱;16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

I'm into brain not brawn.

我用的是大脑不是肌肉

He is all brawn and no brain.

肌肉发达头脑简单。

IGF-1 is also a growth factor—though it promotes brawniness in muscle rather than the production of blood cells.

IGF-1也是一种生长因子,但是它的作用是强健肌肉而不是产生血细胞。

He says he's the muscle of the gang since he's 5'7 and a brawny, heavier guy.

他说,他的肌肉,四人帮,因为他的5'7 和brawny ,较重的家伙。

It's a brawny place - long on muscle, short on shimmer.

这是一个强壮的地方-长期在肌肉,短在淡光。

After two weeks,I found my muscle look much stronger,when I touched them carefully,they were so brawny.

过了两周,我发现自己的肌肉看起来更加强壮了,当我小心翼翼用手去触摸它们时,它们是如此地结实。

He's muscular and brawny.

肌肉发达的,高大的,健壮的

His brawny arms are very strong.

他那肌肉发达的手臂十分有力。

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She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了,但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场,你等着挨绳子吧!

Several theories about reigniting and extinguishing of the arc have been refered.

本文综合考虑了几种电弧重燃和熄灭理论。