肌细胞色素

Under optimal conditions mixtures containing eight inorganic anions, six amino acids such as arginine, histidine, cysteine, methionine, tryptophan and proline with positive or negative charge and four basic proteins such as lysozyme, cytochrome c,α-chymotrypsinogen A and myoglobin were separated completely.

经优化，用中等浓度的中性缓冲液分别成功地分离了八种不同的阴离子Br〓、NO〓、NO〓、SCN〓、MoO〓、BrO〓、WO〓和IO〓；六种带不同电荷的氨基酸如精氨酸、组氨酸、半胱氨酸、甲硫氨酸、色氨酸和脯氨酸；四种碱性蛋白质如溶菌酶、细胞色素c、抗凝乳蛋白酶原A和肌球蛋白。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

PONI skin health research centre, with advanced skin care technology and causes skin pigment, successful research and development of a "'8 'black-suppression, the corresponding treatment and conditioning" technology, not undermine, not suppressed, does not damage the ecology of the skin Under the circumstances, the skin pigment formation of a smart decomposition, hypnosis pigment cells, skin in a relatively short time, reaching Suji elegant, Whitening Source lucent effect, lasting effect, the relative safety of similar products increased 68 percent.

&8&重抑黑，对应疗理，彰显透亮白嫩！柏宁皮肤健康研究中心，结合先进的护肤科技和皮肤色素成因，成功推出&8重抑黑，对应疗理&科技，在不破坏、不压制、不损伤肌肤原生态的情况下，对皮肤色素形成过程进行智能分解，催眠色素母细胞，使肌肤在较短时间内，达到素肌雅致，净白透亮的效果，效果持久、安全性相对同类产品提高了68%以上。

Methods The disorder of myofibrils,swelling of mitochondria with broken cristae and decrease of cytochrome C oxidase activity caused by ADR were prevented by the administration of teapol.

结果 阿霉素所造成的大鼠心肌细胞肌原纤维排列紊乱、线粒体肿胀，嵴断裂及细胞色素C氧化酶活性降低，可明显地被抗氧化剂天力体保所防止。

Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.

应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。

Hemes are cyclic tetrapyrroles that contain iron and commonly found as the prosthetic group of hemoglobin , myoglobin and the cytochromes .

血红素是含铁的环状四吡咯，它作为辅基，普遍存在于血红蛋白肌红蛋白和细胞色素中。

There is an increasing need to better understand the fate of domoic acid, in aquatic ecosystem. In this report, partial cDNA of hepatic cytochrome P-450 1A (CYP1A), giutathione S-transferase alpha, glutathione S-transferase rho, heat shock protein 70 (HSP70), Na~+/K~+-ATPase alpha 1(ATP 1A1) and beta-actin gene from rabbitfish were obtained by PCR using degenerate primers, which respectively were 900 bp, 582 bp, 615 bp, 660 bp, 749 bp and 554 bp in length.

本研究成功克隆了黄斑篮子鱼肝脏第Ⅰ时相去毒酶细胞色素P450 1A，第Ⅱ时相去毒酶谷胱甘肽S-转移酶alpha和谷胱甘肽S-转移酶rho(GS7R)，热休克蛋白70(HSP70)，钠钾ATP酶alpha 1(ATP1A1)和β-肌动蛋白基因的核心序列，它们分别长900 bp，582 bp，615 bp，660 bp，749 bp和554 bp。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。