肌细胞

Collagen, a most abundant protein, is widely distributed in skins, tendons, cartilages and constitutes about 25%-30% by weight of all proteins in human or other animal bodies. Collagen structure is characterized by containing domains with repetition of the Gly-Xaa-Yaa prolinrich tripetide and the trimeric collagen triplehelices.

胶原是动物体内含量最多、分布最广的蛋白质，广泛分布于结缔组织、骨骼、内脏细胞间质及肌腔、韧带、巩膜等部位，占人体或其他动物体总蛋白含量的25％～33％。

Mouse is an ideal experimental animal to research related diseases of myoepitheilal cell of lacrimal glands.

小鼠是理想的研究泪腺肌上皮细胞相关疾病的实验模型。

Methods Twenty cases denervated and 3 cases normal human skeletal muscles were involved in this experiment.

2 0例不同时间人体失神经骨骼肌与 3例正常骨骼肌标本，HE染色和透射电镜观察肌卫星细胞形态变化；抗MyoD和Myf 5多克隆抗体免疫组织化学染色，统计阳性染色细胞核数。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C，在证实其可以在真核细胞中表达之后，将其用脂质转染剂包裹，形成LipofectAMINE-pcDNAHCV-C脂质混合物，将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌，以空载体pcDNA3做对照，ELISA法检测血清中抗体产生水平。

PHBV was implanted subcutaneously in rabbit and extirpated at 2,4,6,8, and 10 weeks in order to study its biocompatibility and biodegradability.

体外培养犬主动脉瓣间质细胞，观察其生长曲线，平滑肌α肌动蛋白表达和透射电子显微镜下特点。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme，HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme，HdvRz)，并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游，构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT，经Plasmid Midi Kit纯化后，在Lipofectamine~ 2000的介导下，直接转染DFI细胞，72h后取上清继续在CEF上传代。

Surgical manipulation can induce inflammatory response within small intestine, stimulate intestinal smooth muscle cells to express iNOS mRNA. As a result, the overproduced NO inhibited the small intestinal motility.

手术操作诱发了小肠肌层炎症反应，并刺激小肠平滑肌细胞表达iNOS，过度产生NO，抑制小肠环行平滑肌的收缩功能。

SCs cultured from rabbit skeletal muscle can grow and maintain their characteristics in vitro provided appropriate passaging rate and culture condition.

从肌SC稳定的生物学特性看，可以将其作为研究肌肉受损后再生修复的细胞模型，用于检测和研究某些基因和生长因子对肌肉再生和功能恢复的影响。

Objective To investigate and verify the regulatory effect of secreting IFN-γ on autoimmune antibody of myasthenia gravis .

目的 了解重症肌无力患者自身抗体的产生与细胞因子的关系。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase II detoxifying genes.

在正常情况下，Kelch样环氧氯丙烷相关蛋白-1 (Kelch-like ECH-associated protein-1， Keap1)可将Nrf2与肌动蛋白细胞骨架一起收集在细胞质内，但是在半胱氨酸残基发生氧化的情况下，Nrf2和Keap1脱离，进入细胞核并与ARE结合，从而激活多种抗氧化基因和II相解毒基因的转录。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。