肌细胞

OBJECTIVE: To observe the influence of the expression of P53 and Bcl-2 protein after incremental training in gastrocnemius muscle and apoptosis of rats with hyperbaric oxygen.

目的：观察高压氧对递增负荷跑台运动后大鼠腓肠肌P53、Bcl-2蛋白表达及细胞凋亡的影响。

Results Compared with low-salt diet control group, the renal changes in CsA-L group were not obvious at the 2nd weeks, the urine volume and the level of BUN were remarkably increased in CsA-H group. At the 4th weeks, the urine volume was decreased; the levels of Crea and BUN were significantly increased and showed a significant positive correlation with the blood CsA concentration. Morphologic changes consisted of vacuolization of renal tubular cells, hyalinization of small arterioles and the light renal interstitial fibrosis.

结果 与低盐对照组相比，药后第2周CsA-L组各指标无明显变化，CsA-H组尿量增多，尿蛋白降低，尿素氮升高；药后第4周时，尿量呈减少趋势，肌酐和BUN明显升高，且与CsA血药浓度呈显著正相关，病理表现为肾小管细胞空泡变性及小动脉和肾间质病变，CsA-H组各种变化较CsA-L组更为明显。

Results CsA clearly decreased creatinine clearance and increased urinary excretion of N-acetyl-β-D-glucosaminidase in rats as compared with those in the vehicle group. Morphologic changes consisted of vacuolization of tubular cell, expansion of the interstitium and interstitial fibrosis, hyalinization of afferent arterioles. Severity of the injury was related to the dosage of CsA. Striped interstitial fibrosis and arteriolopathy were more severe in the rats treated with CsA and low-salt diet than with CsA and normal diet.

结果 用CsA的大鼠出现尿量增多、内生肌酐清除率下降、尿N-乙酰-β-D-氨基葡萄糖苷酶排泄增多等；病理上表现为不同程度的细胞浊肿、空泡变性及灶性分布的间质纤维化和小动脉病变，随CsA剂量的增加，病变呈加重趋势；结合低钠饮食，CsA慢性肾毒性病变明显加重。

Objective To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique.

目的探讨应用改良Percoll非连续密度梯度离心法分离纯化培养组织工程用肌卫星细胞。

Methods The primary skeletal muscle satellite cells of New Zealand white rabbits were cultured with different adhesion time method and incontinuous Percoll density gradient centrif...

分别采用差速贴壁法和Percoll非连续密度梯度离心法进行新西兰兔肌卫星细胞的原代培养并进行纯化。

Results Four weeks after surgery, animals in DO group showed an increased voiding frequency, higher voiding pressure and irregular nonvoiding contractions (n=12, P.05). We found increasing mutual connection and multilayer construction in suburothelium and intermuscular layers of DO but not DS and control bladders.

对照组、DS组和DO组豚鼠膀胱均观察到ckit染色阳性且呈典型长梭形有突起的ICC，主要分布于平滑肌肌束间和黏膜下层；在DO组豚鼠的膀胱中ckit染色阳性的ICC分支突起增加且互相连接呈网络状，而在对照组和DS组中ICC呈散在分布，细胞之间联系较松散。

Of them 25 cases of oral cancer (G1-2) were randomly divided into trail and control group . The remained 11 cases of nasopharyngeal and maxillary sinus cancer (NPC-MSC, mainly G3-4) were attributed to a third group (1st course: sBRM, then 2nd course: IAC). The order of drug-use of sBRM is: pre-immunomodulation of the reactivity of patient low-dose CTX and PolyI∶C, then in- juring tumor and thereby modulation of its immunogeneity (DDP-PYM regimen , thereafter, reaction enhancement modu- lation [low-dose rhuIL-2, Cimetidine and Indomethacin ] . All drugs, except for CTX, PolyI∶C and Indo, were fractionally delivered in a manner of continously infution in 8-10 hours via a retaining intra-external carotid artery catheter CAVAFIX 335 SPLITTOCAN G14, B.

将其中25例晚期口腔高、中分化鳞癌病人随机分为实验治疗（12例）和对照治疗（13例）两组，实验组采用sBRM疗法，具体用药为：先用小剂量环磷酰胺和聚肌胞对机体反应性作预调变，然后用低剂量DDP-PYM化疗杀伤&致创&癌细胞，使其免疫原性增强并借之诱导肿瘤浸润淋巴细胞，化疗结束后续用小剂量rhuIL-2激活和扩增TIL，特别是其中的T细胞，并借助小剂量西咪替丁、消炎痛调节机体以原发灶为中心的癌受创相关反应。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

The results suggest that Galpi may prevent cellular collapse during the desiccation of leucaena seeds and function much the same as oligosaccharides.

由结果推论银合欢种子在乾燥时，甲基肌醇半乳吡喃糖类似寡糖类，也许可防止细胞在乾燥下受到损害。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。