肌细胞

Incubated with cytochalisin B for 4 hours, F-actin bunddles disrupted, confluent ECs monolayer has a complete detachment from the coverslips. So, there is a suggestion that the F-actin in ECs is essential for endothelial adherence under shear stress.

Northcrn杂交结果表明外源性纤维连接蛋白有促进EC肌动蛋白表达的作用，结合F-actin染色及FN受体染色结果，本文认为用纤维连接蛋白作为细胞种植的促粘附物质有助于内皮细胞粘附结构的形成。

Per urinary tract transitional cell carcinoma in patients. Methods:Urinary Fn was detected by ELISA technique in 30 cases with upper urinary tract TCC, 24 cases with other urogenital diseases and 19 healthy subjects. Meanwhile the analysis for creatin in urine was carried out and the Fn/Cr ratio was calculated. The discrepancy of urinary Fn/Cr among three groups and tumor grades and stages was analized as well. Sensitivity and specificity of the urinary Fn/Cr were compared with that of the urinary cytology.

采用ELISA法检测30例上尿路移行细胞癌、25例其它泌尿系疾病患者及20例正常人尿液Fn含量，并同时测定尿肌酐，计算Fn/Cr比值，比较各组人群尿Fn/Cr比值之间的差异；分析不同肿瘤分期、分级尿Fn/Cr之间的差异；比较其与尿脱落细胞学检测上尿路移行细胞癌的敏感性。

Focal solution of muscle fiber,the aggregation of mitochondria around the nucleus,the increase of lipid droplet and lysosome in cyte,and the proliferation and differentiation of muscle satellite cell could be observed with an electron microscope.

结果］各项酶学检验无异常；组织学光镜下观察到竖脊肌纤维部分溶解变性，肌纤维肥大，少量炎性细胞浸润；电镜下观察到肌纤维灶状溶解，核周线粒体聚集，胞内脂滴、溶酶体增多，肌卫星细胞增殖分化。

Detail Contents: Genetic disorders -- Immune deficiencies -- Breast cancer -- Colon cancer -- Melanoma -- Cystic fibrosis -- Hemophilia -- Liver disease -- Cardiovascular disease -- Muscular dystrophy -- Alzheimer's disease -- Parkinson's disease -- Huntington's disease -- Viruses: the cornerstone of gene therapy -- Viruses are living crystals -- Viral genomes may be RNA or DNA -- Viruses evolved from plasmids -- Viruses know how to infect cells -- The virus as a gene vehicle -- Viruses used in gene therapy -- Ashi DeSilva: a promising start -- Clinical trials defined -- Cells of the immune system -- Adenosine deaminase -- Preliminary research -- Clinical procedure for ADA gene therapy -- The DeSilva clinical trial -- Jesse Gelsinger: down to earth -- Ornithine transcarbamylase -- Preliminary research -- Clinical procedure for OTC gene therapy -- The Gelsinger clinical trial -- The investigation -- Concluding remarks -- Future prospects -- Safer vehicles -- Reducing immune rejection of the vector -- Improved risk assessment -- Redesigning human anatomy and physiology -- Ethics of gene therapy -- The Belmont report -- Clinical trials -- Physiological enhancement -- Cosmetic applications -- Legal issues -- Regulatory agencies -- The Gelsinger legal trial -- International regulation -- Resource center -- Eucaryote cell primer -- Recombinant DNA primer -- The human genome project -- X-linked severe combined immunodeficiency (SCID-X1)-- Alzheimer's disease -- Huntington's disease.

细节内容︰遗传疾病-免疫的缺乏-乳腺癌-结肠癌-黑瘤-囊性纤维变性-血友症-肝疾病-心血管疾病-肌营养不良-早老性痴呆病-帕金森疾病-亨廷顿疾病-病毒︰基础的基因治疗-病毒在活著水晶--病毒的基因可能是RNA或者DNA --病毒从plasmids被逐步形成--病毒知道怎样感染细胞--作为一辆基因车辆的病毒--基因治疗使用的病毒-Ashi DeSilva︰有希望开始-临床试验确定--细胞的这免疫系统-Adenosine deaminase-初步研究-临床程式给埃达基因治疗--这DeSilva临床试验-婕西Gelsinger︰到地球-Ornithine transcarbamylase-初步研究-临床程式给OTC基因治疗-- Gelsinger临床试验-调查-达成评论-前景-更安全的车辆--矢量的降低免疫的拒绝-改进风险估计-重新设计人解剖学和生理学--伦理学的基因治疗-那些贝拉蒙特报告-临床试验-生理提升-美容应用-法律问题-协调机构-- Gelsinger 合法审讯-国际管理-资源中心人物-Eucaryote信元第一-Recombinant DNA 入门--人类基因工程-- X 连结的严重的结合的免疫缺陷(SCID-X1)-早老性痴呆病--亨廷顿的疾病。

Actin, as a component of the complex skeleton in the eucaryotic cell, takes part on many important cellular functional progresses.

肌动蛋白是真核细胞骨架的主要成分，与细胞内的许多重要功能活动有关。

Actin is a kind of highly conserved protein,existing in all eukaryote.

肌动蛋白是一种高度保守的蛋白质，存在于所有真核细胞中，参与细胞分裂、运动、迁移、形态的维持、生长等多种重要生理活动。

Furthermore,we observed that TH1 localized to focal adhesions and filopodia in the leading edge of cells,where TH1 reduced cell migration through affecting actin and adhesion dynamics.

此外，我们还观察到TH1定位于迁移细胞前沿的粘附斑和纤维伪足上，并且通过影响肌动蛋白和粘附动力学变化干扰了细胞的迁移。

Cdc42 and Rac1 have similar effects on cytoskeleton. They both promote the actin polymerization in the cell and profit the formation of filopodia and lamelipodia respectively.

Cdc42和Rac促进细胞内肌动蛋白聚合，分别有利于丝状伪足和片状伪足的形成；而RhoA可能具有与Cdc42 和Rac相反的效果，通常造成细胞边缘和突起的收缩及胞体变圆。

In the rat carotid injury model, SM16 dosed once daily orally at 15 or 30 mg/kg SM16 for 14 days caused significant inhibition of neointimal thickening and lumenal narrowing. SM16 also prevented induction of adventitial smooth muscle alpha-actin-positive myofibroblasts and the production of intimal collagen, but did not decrease the percentage of proliferative cells.

在大鼠颈动脉损伤模型中，每日口服15或30mg/kgSM16持续14天能够显著抑制新生内膜增厚及管腔狭窄，预防对外膜α-SMA阳性肌成纤维细胞的诱导和内膜胶原的合成，但并不降低增殖细胞的比例。

We employed a cell line of mouse podocytes immortalized with the simian virus 40 large tumor antigen gene, and examined the cell proliferation using colony-forming and MTT assays following treatment with various concentrations of bFGF. BMP-7, alpha-smooth muscle actin e podocytes were detected by western blot. Methyl blue staining was used for the observation of cell morphology.

利用SV40肿瘤抗原转染所获得的永生性小鼠足细胞系培养，以集落形成法和噻唑蓝MTT还原实验测定不同浓度bFGF刺激下的足细胞增殖，采用SDS-聚丙烯酰胺凝胶电泳和Western blotting 检测BMP-7、alpha-平滑肌肌动蛋白、以及波形蛋白的蛋白质水平表达，同时以甲基兰染色观察细胞形态。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。