肌细胞

Results With the expression in normal salivary gland as a standard, low C-erbB-2 mRNA expression was seen in adenolymphoma, basal cell adenoma. However, various degrees of over-expression of C-erbB-2 oncogene mRNA were detected in pleomorphic adenoma, mucoepidermoid carcinoma,acinic cell carcinoma ,adenoid cystic carcinoma ,papillary cystic carcinoma,and myoethelial cell carcinoma.

结果 正常涎腺和涎腺肿瘤中发现不同程度C-erbB-2mRNA的表达，以正常涎腺组织的表达水平为基准，在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或与正常涎腺组织表达水平相似，多形性腺瘤、粘液表皮样癌、腺泡细胞癌、腺样囊性癌、乳头状囊腺癌和肌上皮癌则呈现不同程度的过表达。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

Angiectasis, permeability were increased , inflammatory cell were transuded; The cardiac muscle injury conditions after hypoxia 4 h were similar with hypoxia 2h group, appeared lipid droplet, implied the oxidative metabolism of lipid got obstacle started from then; The cardiac muscle injuried conditions were got little improvement in hypoxia 6 and 8 hours team, mitochondria were increased and aggregatived, normal cell junction, sarcomere contract weaked; in hypoxia 10h group , mitochondria were increased and aggregatived obviously, a few glycogenosome were assembled, transverse tubule were expanded, all the changes may be the adaption of Gansu zokors in the hypoxia tolerance process to 4% hypoxia extent gradually.

在4%严重低氧耐受过程中，甘肃鼢鼠心肌超微结构在2h组出现血管扩张，通透性增加，有炎细胞渗出，异染色质边集；4h组心肌损伤状况与2h组接近，出现脂滴，提示脂类的氧化代谢在此时开始发生障碍，6和8h组心肌损伤状况逐渐好转，线粒体聚集增多，细胞连接正常，肌小节收缩较前有所减弱；10组线粒体增多聚集，线粒体脊增粗更为明显，少量糖原颗粒聚集，脂滴较前有所减少，横小管扩张增大变粗，出现了与能量代谢有关的结构适应性变化。

Methods: Tissue of rat ovary was obtained from 30 healthy sexually mature non-mated femal SD rats. The rats were divided into two groups (oestrus group and anoestrus group). The expression of α-SMA and vimentin were detected and evaluated by immunohistochemistry and image analysis system in the theca cells of different type follicles.

分别取动情期和非动情期大鼠卵巢，运用免疫组化方法检测肌成纤维细胞的特征性标记和波形蛋白分别在这两组中各级卵泡卵泡膜细胞上的表达情况，并进行图像分析。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落，不连续，弹力纤维自溶；动脉瘤血管壁均可见SMC明显减少，较多SMC染色质边聚、固缩，部分SMC核全部固缩或出现不规则凹陷，SMC核胞浆或胞核部分出现脱落或裂解成碎片，晚期可见典型的凋亡小体出现，肌丝大都自溶消失：外膜可见大量的胶原纤维，排列较紊乱；瘤壁还可见炎性细胞浸润。

Results The tissue of 3 cases of ACC in the esophagus was mainly composed of square shape gland epithelial cell and variant myoepithelial cell, mesh shape cavity crack and the tubular, the entity shape arrangement, the cavity crack content basophilia and acidophilia the secretion. There was transmigration of superficial epidermis in 1 case of ACC; All 3 cases were positive of CK, H-CK, P63, S-100 (endochylema /nucleus), EMA, and Bcl-2. Case 3 was positive of CerbB-2 and P53. Case 2 was CD117, AAT, SMA, PAS/AB.

结果 3例食管腺样囊性癌的癌组织主要由立方形腺上皮细胞和变异的肌上皮细胞组成，呈筛孔状腔隙、管状、实体状排列，腔隙内含嗜碱性和嗜伊红分泌物；3例食管腺样囊性癌中例1表面上皮有移行；免疫组化：3例CK、H-CK、P63、S-100、EMA、Bcl-2均，例3 CerBb-2，P53，例2 CD117，AAT、SMA，PAS/AB。

SMA staining was positive in basophilous small blocks, spindle or irregular cell cluster. Of the 218 cases, 56 cases had smooth muscle. S-100 staining demonstrated that neurofilament protein and adipocytes were positive. Of the 218 cases, 44 cases had adipose tissue.

平滑肌肌动蛋白染色显示嗜碱性的小团状、梭形或不规则形的细胞束呈阳性反应，证明为平滑肌，218例中56例显示了平滑肌的存在。S-100染色神经纤维丝及脂肪细胞均呈阳性反应，218例中44例有脂肪组织。

The function of bile canaliculi was assessed by observation of the tran...

E2促进大鼠三维培养的肝细胞胆汁分泌，在RhoA和radixin介导下肌动蛋白重新分布，细胞骨架再建，调节紧密连接功能和聚集，导致毛细胆管样结构的扩张和三维培养时球状体空泡样结构的形成。

Concludes: Tryptase and/or chymase contents and subtypes of mast cell are related to intracellular F-actin ring in the tissue of human skin.

人皮肤组织中肥大细胞内类胰蛋白酶和/或类糜蛋白酶的含量及其肥大细胞亚型与丝状肌动蛋白环相关联。

The purpose of our study is to investigate the mechanism of Cinnamyl aldehyde on NIH3T3 proliferation and collagen synthesis at an angle of cell biology. 1.Effects Of CA On NIH3T3 Proliferation And collagen synthesis (1) Cell proliferation was measured by MTT assay.

本研究以NIH3T3为细胞模型，从细胞生物学角度探讨肉桂主要有效成分桂皮醛的作用机制，为&回阳生肌法&提供理论依据，并为肉桂在中医外科治疗慢性皮肤溃疡过程中的推广应用提供药理学基础。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。