肌细胞

The commonest protein in muscle; a globulin that combines with actin to form actomyosin.

在肌肉细胞中最普通的蛋白质，与肌动朊结合形成肌动球朊。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料，从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用，并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

The xerogel promoted the differentiation and conglomeration of C2C12 cells (C3H mice muscle cells), which revealed good biocompatibility of the material.

体外细胞培养表明：硅基干凝胶可促进C2C12细胞（C3H小鼠肌原细胞）的分化和繁殖，证明材料的生物相容性良好。

Results: Compared with control dogs, sections from AF dogs showed cellular hypertrophy, disarranged cell bundles and increasing connective tissue.

结果：与对照犬相比，AF犬肺静脉肌袖内心肌细胞增大、排列紊乱；细胞核大小不甚规则，核异型性明显，细胞内可见肌纤维断裂；心肌纤维之间连接组织积聚，使心肌细胞之间的间隔增宽。

Methods: the cells are isolated by two-step enzyme digestion and purified by the Percoll density centrifugation,the proliferation and differentiation propertiesof which being observed.To study the effect of IGF-1 on satellite cell with MTT method and obtain adequate single satellite cell by frequent passaging.

运用两步酶消化法分离卫星细胞，不连续密度梯度离心法纯化后体外培养，观察其生长与分化特性，运用MTT法测定卫星细胞对不同浓度IGF刺激的反应，并运用频繁传代的方法获取足量单个肌卫星细胞。

The digestions should be less than 6 times, and every digestion should last for less than 20 min. Two-hour differential adherence could improve the purity of myocardial cells. By this protocol, 94.6% of cultured cells were live myocardial cells.

分离细胞时消化次数最好控制在不多于6次，每次消化时间不超过20min.2h差速贴壁可以提高心肌细胞纯度，经肌动蛋白鉴定，心肌细胞达94.6%。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素（bleomycin，BLM）复制大鼠肺纤维化模型，并分离培养肺成纤维细胞，用阿托伐他汀对其进行干预，分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组，用倒置纤维镜观察细胞的形态结构，MTT计数法观察各组细胞的增殖情况，免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ，α-SMA)的表达情况。

MyosinⅩ, a vertebrate-specific member of myosin super-family, can be detected in most tissues and cells, such as kidney, liver, brain, and so on. Full-length MyosinⅩcan stimulate the formation or stabilization of filopodia. As an actin-based motor protein, MyosinⅩplays a key role connecting cell membrane proteins and cytoskeleton.

肌球蛋白Ⅹ是脊椎动物所特有的细胞骨架蛋白，在脑、肝、肾等大多数组织中都有表达，它的显著特点是定位于细胞丝足的顶端和片状伪足的边缘，MyosinⅩ作为一个动力蛋白分子，起到连接细胞膜蛋白与细胞内骨架蛋白的桥梁作用，因此推测它可能在SVZa成神经细胞的迁移过程中起到重要作用。

MTT assay was used to evaluate the cytotoxicity of β-TCP/col on the proliferation of PDLCs. PDLCs were cultured on β-TCP/col and observed by scanning electron microscope.β-TCP/col was implated into muscles of nude mice to observe tissue reactions by histologic methods.Ⅱ° furcation involvements models in beagle dogs were established, scaffold and and scaffold with cells and cytokines were implanted into defects. Histologic observation were conducted.

通过 MTT 法测定β-TCP/col 对犬 PDLCs 增殖的影响，在β-TCP/col 上复合培养犬 PDLCs 并进行电镜观察；将β-TCP/col 植入裸鼠肌袋内并进行组织学观察；建立比格犬Ⅱ°根分叉病变模型，分别植入材料、材料/细胞复合体或材料/细胞/细胞因子复合体，进行组织学观察。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。