肌细胞

Endothelial cell form and contractility are regulated by cytoskeleton proteins,such as actin and myosin.

内皮细胞形态变化和收缩性的改变主要受细胞骨架蛋白如肌动蛋白和肌球蛋白的影响。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

Results: MC of tryptase immunoreactivity contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase temporarily was stored in secretory vacuole, and small amounts tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

Results: MC of tryptase immunoreactivity MC(subscript T contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase was temporarily stored in secretory vacuole, and small amounts of tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

The changes included that the protrusions on the cell surface diminished, myofilaments and pinosomes in the myoepithelial cells of grade Ⅱ,Ⅲ atypical hyperplasia decreased, and the irregularity of the nuclear morphosis and the increase of nuclear heterochromosome were found.

乳腺不典型增生时肌上皮细胞数目增多，排列紊乱，电镜下可见：①细胞表面的棒状突起消失；②Ⅱ、Ⅲ级不典型增生时胞浆内的肌微丝和吞饮小泡减少；③细胞核增大，不规则，异染色质增多。

The distributions of NOS in the pylorus were similar in both groups.

患儿组贲门部肌层中缺乏 NOS 染色阳性神经纤维，肌间神经节细胞呈 NOS 染色阴性，远端食管及胃底部偶可见散在的 NOS 染色阳性神经节细胞，幽门部 NOS 的分布与对照组基本相同。

The cardiomyocyte viability were detected by MTT assay for calculating the survival rate and the inhibitory rate of the virus. Myocardial F-actin and VEGF were analyzed by immunofluorescent staining with confocal microscopy. Mean fluorescent intensity of F-actin and VEGF were determined by flow cytometry.Results There is no toxicity on cardiomyocytes when PD is at concentration of 0.02 mmol/L and 0.2 mmol/L while there is toxicity at concentration of 2 mmol/L. CVB3 could reduce the viability of cardiomyocytes, depolymerize F-actin cytoskeleton and reorganize VEGF protein.

体外培养心肌细胞，用100 TCID50柯萨奇B3病毒（CVB3）感染心肌细胞，建立病毒性心肌炎实验模型，然后分别用0.02mmol/L、0.2mmol/L、2mmol/L三种不同浓度的PD处理感染CVB3病毒的心肌细胞，观察心肌细胞的形态和搏动，用细胞免疫荧光技术标记纤维状肌动蛋白和VEGF蛋白，四唑蓝比色法测定心肌细胞活性和病毒抑制率，流式细胞仪检测各组心肌细胞VEGF蛋白和F-actin平均荧光强度。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

At postnatal 1st week immunopositive reaction of NGF was detected mainly in sustentacular cells and the spermatogonia also showed positive staining. NGF positive staining in the testes was observed in interstitial cells, spermatogenetic cells, sustentacular cells and Leydig cells at 3rd week. After the postnatal 5th week, NGF-positive immunostaining was also detected in intersitial cells and spermatogenetic cells, but the intensity of reaction was weaker than that at 1st and 3rd weeks.

免疫组化定位分析显示：睾丸组织的神经生长因子蛋白表达于小鼠出生后的各个时期内，1周龄睾丸组织免疫阳性反应主要位于支持细胞，精原细胞也有着色；3周龄睾丸组织的间质细胞、各级生精细胞、支持细胞、管周肌样细胞表达均呈现阳性；5周后的睾丸组织内神经生长因子呈低水平表达，主要表达于间质细胞和生精细胞内。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。