肌细胞

There was a great reduction of volume of nucleus pulpous cells following loading, and most of the actin in nucleus pulpous cells assembled around its membrane.

体外培养的单层椎间盘细胞在受力状态下，肌动蛋白的表达减少，细胞的体积缩小，并且肌动蛋白在细胞内重排列。

Results showed that L-THP possessed negative inotropic and negative chronotropic action, lowed left ventricular pressure and it's infinitesimal calculus, decreased heart output in per min., shorten 〓 and 〓 of action potential of ventricular papillary muscle of guinea pig and even the plate-phase of action potential disappeared in larger dose group , but prolonged 〓. we studied transmembrane transportation of 〓 after administering L-THP , L-THP not only depressed uptake and increased release of Ca 2+ in normal myocardium, but also it showed stronger inhibition in cell injured by ISO, and fully eliminated the effect of ISO increasing uptake and decreasing release of 〓. By detecting the 〓 content of ventricular single myocyte using fluorescent probe Fluo-3/AM, results correspond to that done above, L-THP decreased 〓 content inside of cell significantly. Using whole-cell mode, we observed the effect of L-THP on Ica in ventricular single cell, it was found that L-THP decreased Ica, the effect increased with increasing it's dose, and had voltage, frequency-dependency. Dose highly corelated to effect, corelative constant is 0.87. When washing cell with normal tyre's solution, Ica could recover, which proved the blocked action of L-THP on calcium channel once again. Meanwhile, L-THP reduced production of MDA in heart injured by ISO in dose-dependent manner, improved content of GSH-Px, in case tissue or cell free from free radical damage.

结果显示，该药物具有负性肌力和负性频率作用，降低左室压及其微分，终使每分心输出量减少；缩短豚鼠心室乳头肌动作电位时程的〓和APD〓，大剂量使动作电位平台消失，〓延长；同位素示踪法研究证实L-THP抑制〓摄取，促进〓释放，降低细胞内的〓含量；单通道水平的研究证实，L-THP减少豚鼠心室肌单细胞内向钙电流，且具有剂量，电压，频率依赖性，其作用易于洗脱，药物剂量与Ica也具有高度相关性（相关系数r为-0.87）；L-THP还可抑制ISO损伤心肌组织所致的丙二醛含量增加，增加谷胱甘肽过氧化物酶的量，使组织细胞免受损伤。L-THP阻断L-型〓通道，抑制〓的摄取，促进〓的释放，降低细胞内〓含量L-THP作用的离子基础。

NGFR positive nerve fibres were markedly reduced or absent in muscular layer and submucous layer while the hypertrophic nerve trunks in myenteric and submucosal plexuses showed a sheath of intense staining in the aganglionic colon from patients with HD.

结果 丰富的NGFR染色阳性神经纤维分布于正常结肠环肌层及粘膜下层，少量分布于纵肌层，NGFR染色阳性神经元分布于肌间神经丛及粘膜下神经丛；NGFR 染色阳性神经纤维在HD无肌间神经节细胞肠段肌层及粘膜下层内明显减少或缺如，而肌间肥大的神经干及粘膜下神经干为NGFR染色阳性，并且在其外周可见一强阳性染色带。

Now we don't know how aminoglycoside antibiotics damages hair cells, but there are some theories about it, such as:(1)AmAn affects the transformation among phosphoinositoln, inositol- 3-phophate and glycerine-2-acyl, also, it can cause the change of Ca2+ concentration in endocellular.

氨基糖甙类抗生素(aminoglycoside antibiotics， AmAn)损伤毛细胞的机制尚不清楚，但有以下几种学说：(1)影响了磷酸肌醇、三磷酸肌醇、二脂酰甘油间转化从而导致细胞内钙离子浓度的变化；(2)抑制G蛋白和鸟氨酸脱羧酶活性；(3)氨基糖甙类抗生素能引起内耳毛细胞的凋亡。

Part I: Primary culture and purification of skeletal muscle derived satellite cells for tissue engineering applications To investigate separation and purification of skeletal muscle satellite cells with improved incontinuous density Percoll gradient centrifugation technique for tissue engineering applications.

第一部分：种子细胞—肌卫星细胞的原代培养及鉴定本部分通过应用改良Percoll非连续密度梯度离心法分离纯化培养新西兰兔骨骼肌干细胞—肌卫星细胞，探讨为组织工程膀胱的构建提供种子细胞的可行性。

Therefore, the disruption of the actin cytoskeleton initiates a cascade of structural and functional alteration in renal epithelial cells and which will lead to the dysfunction of reabsorption in kidney.

肌动蛋白聚合形成了肾脏上皮细胞内对微绒毛起支撑作用的细胞骨架，因此肌动蛋白细胞骨架的破坏势必导致肾小管上皮细胞结构和功能的改变，从而引起肾脏的重吸收功能障碍。

Methods Employing cholera toxin B-subunit combined horseradish peroxidase retrograde tracing technique and cholera toxin B-subunit combined colloid goldretrograde tracing with calcitonin gene-related peptideimmunohistochemical double-labeled staining technique,the innervation of musculus sacrococcygeus ventralis medialisin rats was studied.

大鼠骶尾腹内侧肌又称荐尾腹侧中肌(musculus sacrococcygeus ventralis medialis)或屈尾短肌，是位于盆腔后壁、骶椎前方的一对梭形肌肉，属尾肌群，因其功能似乎仅为屈尾，故至今尚未引起人们的注意，而与该肌位置关系较密切的会阴肌因在某些运动神经元疾患时仍可保持其正常生理功能而一直受到神经工作者的关注，尤其是近年来有诸多文献报道了会阴肌的神经支配、相关神经元的细胞构筑、神经递质和神经调质的分布以及纤维联系特点［1～10］，藉以探讨运动神经元疾病的神经病理学。

MSC induced by 5-aza could be identified by the positive staining for Troponin I and Connexin43. The number of cells stained positively in the 10umol L-1 and 20umol L-1 5-aza was visually greater than that of other groups , but more than 50% the cells necrosed in 20umolL -1 5-aza. Electron microscopy revealed sarcomeric organization and gap junction.

结果MSCs体外贴壁生长，呈成纤维细胞样形态，经5一aza诱导后细胞体积增大，变为球状或者长杆状，4w后荧光免疫细胞化学方法检测肌钙蛋白、肌动蛋白及Connexin43表达阳性，10umol·L一'5一aza组及20umoz·L一'5一aza组阳性表达较高，但20umol·L一'5一aza组约50%细胞死亡。

Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化；RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化；抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达，包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases， ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor， Rho-GDI alpha)，研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4～5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果：模型C组于免疫注射第3周左右开始出现活动减少，倦怠嗜卧，食欲体重下降等表现，模型B组有14只SD大鼠于免疫注射第4～5周出现上述症状，较C组为轻；模型组肌酶谱中，肌酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高，模型C组较模型B组升高更显著；模型组肌电图时限缩短，波幅降低，多相波增多；磁共振检查模型B组和C组选送标本中各有一例有阳性改变，表现为T1MI等或稍低信号，T2MI及STIR序列为高信号，提示肌肉炎症水肿改变；模型B组有11只，模型C组全部大鼠骨骼肌出现病理改变，表现为横纹肌局灶性分布的肌纤维变性炎细胞浸润，间质小血管壁增厚、扩张，随机选送的心肌标本中有3例有阳性改变，表现与骨骼肌相仿，选送的肺标本中有1例有阳性改变，表现为蛋白渗出，炎症细胞浸润和小血管改变。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。