肌细胞

In order to evaluate the effects of calcium ion on calpalns mRNA expression and proteolysis activity,and muscle protein degradation,L6 myoblast and beef fascicle were used in vivo and in vitro samples respectively in this study.

本实验分别以牛肉肌束和分化为肌细胞的L6成肌细胞为研究对象，研究钙离子对离体肌束中钙激活蛋白酶活性、蛋白分解以及活体肌肉中钙激活蛋白酶mRNA水平表达的影响。

In conclusion, the results indicated that the calpastatin level was modulated by certain growth and differentiation factors and its continuous presence resulted in the inhibition of myoblast fusion. Calpains may play an important role in cytoskeletal reorganization that is requisite for myoblast fusion.

结果表明，细胞内calpastatin的含量是由某些生长与分化因子调控的，calpastatin的连续出现抑制了成肌细胞的融合，说明calpains在成肌细胞融合进程中，对细胞骨架的重组起关键性地作用。

The effect of leptin on promoting myoblast proliferation and cell accumulation in the S+G2/M phase were inhibited by TNF-α.

脂肪细胞因子TNFα抑制leptin对猪骨骼肌成肌细胞增殖和增殖期S+G2/M阶段细胞积聚的促进作用；降低leptin对猪骨骼肌成肌细胞分化期间对CK活性和MyoG表达的抑制效应。

Myoblast that isabundent and easy to get can fuse with the local myoblast to formpolynuclemyoblast and live in myofiber after transplantation.

成肌细胞数量丰富，易于获取，可以通过注射进行大规模细胞移植，可与宿主的肌肉细胞融合形成多核肌细胞，并可以在肌纤维中存活。

Fund Project:Tackle Key Program of Liaoning Province, No. BACKGROUND: Duchenne muscular dystrophy is a cureless disease, it is a severe result of progressive atrophy of muscle cells, which caused by various defects.

背景：杜氏型肌营养不良的肌细胞损伤并非单一细胞受损，而是多因素缺陷导致肌细胞进行性萎缩的严重后果，患儿一经确诊即被告之无法医治。

Finally, we also examined the diferentiation efect of Ad-GFP on myoblasts.3.Study of the transfection of Ad-GFP to myoblasts which Transplanted in the intrarectal of the immune defect rat.The immune defect rats Were randomized in to 2 groups( 10 in each group):The experimental group transplanted with myoblasts transfected by Ad-GFP; the control group injected PBS.

并观察转染了Ad-GFP后是否会对成肌细胞的分化融合有影响。3、转染Ad-GFP的成肌细胞在免疫缺陷小鼠直肠内移植的实验研究将20只免疫缺陷小鼠随机分成2组(每组10只)，移植组肛管直肠粘膜下层注射转染Ad-GFP的成人成肌细胞，对照组注射PBS。

Myoblast differentiation and fusion in multinucleated muscle cells was studied in myoblasts growth in culture. The content ofcalpastatin in muscle wonld be related to cell membrane fusion.

在成肌细胞培养中，成肌细胞融合并分化形成多核肌细胞，其中钙激活酶抑制蛋白的含量与细胞膜的融合相关。

C2C12 cells were induced to differentiation into muscle cells in vitro and the myogenesis model was detected by inverted microscope, RT-PCR, western-blot, cell immunofluorescence histochemistry, flow cytometry and confirmed to be successful. On the foundation of differentiation model, miR-206 was detected by northern hybridization and the result showed that the expression level increasing gradually during muscle differentiation with a character of phase specificity.

利用C2C12细胞在体外成功诱导其向成肌细胞分化，通过形态学观察，RT-PCR、western-blot、细胞免疫荧光组织化学及流式细胞仪等方法进行检测，证实我们所建立的细胞分化模型是成功的，并观察到随着诱导时间的延长，miR-206在成肌分化过程中表达量逐渐增高的，表达改变具有阶段特异性。

Extracellular calcium is required for normal progression through the initial recognition-alignment phase of myogenesis. A net calcium influx into fusion-competent myoblasts is a requisite step in membrane fusion.

细胞与细胞之间识别和并列的初期阶段，胞外钙离子的内流是正常肌细胞生成过程所必不可少的，钙离子进入具有融合潜能的成肌细胞内，从而使细胞膜发生融合。

Desmin, the marker of myoblasts, was adopted to identify the expression of specific marker protein of sarcoblast desmin with immunohistochemistry. Desmin negative cell clones were removed, and desmin cell clones were cultured continuously with the culture fluid replaced once every other day.

采用成肌细胞特异性标志抗原desmin免疫化学染色，鉴定成肌细胞标志蛋白——结蛋白的表达，弃去desmin阴性的细胞克隆，继续培养desmin阳性的细胞克隆，隔天换液1次，7 d进行酶消化传代，获得大量扩增的细胞，并可冻存复苏，用于实验。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。