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14Th week after injury, the reorganization of labelled neurons' distribution was found in the autograft group, but only one case in the NTN/PGLA group. Conclusion Local application of PGLA tube with neurturin can recover the axoplasm flow of regenerated nerve fibers, its long-term effect can match that of the nerve autograft, and collateral axonal branching occurred less.

应用NTN/PGLA导管的修复能够和自体神经移植修复一样恢复神经缺损的连续性和逆行轴浆运输功能,后期效果相当;应用NTN/PGLA导管修复后神经元分布异位明显少于自体神经移植修复,提示较少发生轴突误向再生,减少面肌联带运动的发生。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落,不连续,弹力纤维自溶;动脉瘤血管壁均可见SMC明显减少,较多SMC染色质边聚、固缩,部分SMC核全部固缩或出现不规则凹陷,SMC核胞浆或胞核部分出现脱落或裂解成碎片,晚期可见典型的凋亡小体出现,肌丝大都自溶消失:外膜可见大量的胶原纤维,排列较紊乱;瘤壁还可见炎性细胞浸润。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

The anatomy relationship of venae hepaticae intermediae and its branches on gallbladder bed were analysed,which could be the reference during the LC.Results:For 112 patients suffered from inatrophic cholecystitis and cholecystolithiasis,whose gallbladders were decoherence directdly from ectoptygma muscular coat close to the gallbladder wall.

结果:对于术前经彩色多普勒超声波检查明确的肝中静脉直接和胆囊床相贴的非萎缩性胆囊炎胆囊结石的102例患者,采用紧靠胆囊壁的浆肌层直接进行剥离;而慢性萎缩性胆囊炎胆囊结石的24例患者,采用胆囊粘膜切除,或者直接开腹手术切除,未发生因损伤胆囊床肝中静脉而大出血。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

The history is important to the diagnosis of CICA.The key of saving life is the decompression peritoneocentesis in time.The local treatment of colorectal injury depends on routine of colorectal injury treatment.But if there are some conditions that affect the colorectal transit,the colon should be shortened properly to defecate facilely.Key words colorectal injuries compressed air abdominal pressure

病史对CICA的诊断重要;及时的腹穿减压是挽救生命的关键;压缩空气所致的大肠局部损伤可按常规处理,但如存在浆肌层广泛剥离等影响大肠传输功能时,应适当缩短结肠,使肠内容排出容易。

Kelch-like ECH-associated protein-1 (Keap1) normally sequesters Nrf2 in the cytoplasm in association with the actin cytoskeleton, but upon oxidation of cysteine residues Nrf2 dissociates from Keap1, translocates to the nucleus and binds to ARE sequences leading to transcriptional activation of antioxidant and phase Ⅱ detoxifying genes.

在正常情况下,Kelch样环氧氯丙烷相关蛋白-1(Kelch-like ECH-associated protein-1, Keap1)与Nrf2耦联,并与肌动蛋白细胞骨架结合被锚定于胞浆,但是在半胱氨酸残基发生氧化的情况下,Nrf2和Keap1解耦联,进入细胞核并与ARE结合,从而激活多种抗氧化基因和Ⅱ相解毒酶基因的转录。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

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