肌浆

Objective To study the effects of matrine on intracellular calcium [Ca(superscript 2+] in single guinea-pig ventricular myocytes.

目的 研究中药苦参碱对豚鼠心室肌细胞胞浆钙的影响。

Methods The dynamic calcium concentration in single guinea-pig ventricular myocyte was measured by laser scanning confocal microscope.

应用激光扫描共聚焦显微技术动态测量单个豚鼠心室肌细胞胞浆钙的浓度。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落，不连续，弹力纤维自溶；动脉瘤血管壁均可见SMC明显减少，较多SMC染色质边聚、固缩，部分SMC核全部固缩或出现不规则凹陷，SMC核胞浆或胞核部分出现脱落或裂解成碎片，晚期可见典型的凋亡小体出现，肌丝大都自溶消失：外膜可见大量的胶原纤维，排列较紊乱；瘤壁还可见炎性细胞浸润。

Results Eight cases all cured, thereinto, one stitches removed, one ureter membrane repaired, by cystoscopic reversing ureter, two diversion by embedding D-J pipe, one ureter anastomosed, one kidney cutted, two bladder repaired.

结果 8例均治愈，其中拆除缝线1例，行输尿管浆肌层修补1例，经膀胱镜逆行输尿管植入D-J管引流2例，输尿管端端吻合1例，肾切除1例，膀胱修补2例。

Under fluorescence microscope, cytolymph could emit red fluorescence with Rhodamine, and cellular nucleus could emit blue fluorescence with DAPI, accompanying myofilament structure.

免疫细胞化学检测：荧光显微镜下，罗丹明染色细胞浆呈红色荧光，并可见肌丝结构；DAPI染色细胞核呈蓝色荧光。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The anatomy relationship of venae hepaticae intermediae and its branches on gallbladder bed were analysed,which could be the reference during the LC.Results:For 112 patients suffered from inatrophic cholecystitis and cholecystolithiasis,whose gallbladders were decoherence directdly from ectoptygma muscular coat close to the gallbladder wall.

结果：对于术前经彩色多普勒超声波检查明确的肝中静脉直接和胆囊床相贴的非萎缩性胆囊炎胆囊结石的102例患者，采用紧靠胆囊壁的浆肌层直接进行剥离；而慢性萎缩性胆囊炎胆囊结石的24例患者，采用胆囊粘膜切除，或者直接开腹手术切除，未发生因损伤胆囊床肝中静脉而大出血。

Gallbladder endoscopicmucosalresectionor open cholecystectomy were applied in 28 cases developed with chronic atrophic cholecystitis and cholecystolithiasis.No hemorrhoea resulted from venae hepaticae intermediae on gallbladder bed injury were occured in all the cases.

术前常规彩色多普勒超声波检查，明确胆囊床肝中静脉及属枝位置关系，对于肝中静脉与胆囊床相贴的病例，采用紧靠胆囊壁的浆肌层剥离，或采用胆囊粘膜切除，或直接开腹手术切除，可以避免因损伤胆囊床肝中静脉而导致大出血。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

Overloaded rat models were established by using abdominal aorta constriction method. The levels of creatinine and urea nitrogen in the rats?? serum as well as those of malonaldehyde and glutathion of the rats?? nephric tissue were measured respectively.

采用腹主动脉缩窄法制备压力超负荷大鼠模型，分别测定大鼠血清肌酐和尿素氮含量及大鼠肾组织匀浆丙二醛、谷胱甘肽含量。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。