肌动蛋白

The cloning and sequence analysis of Chinese human cardiac myosin light chain 1 (CCMLC1) was previously reported.

肌肉收缩的分子基础在于肌球蛋白头部和肌动蛋白的结合，而结合于肌球蛋白头部的轻链则起收缩的调控作用。

In chapter one, the molecular architecture of the sarcomere, the structures of myosin and actin, and the mechanochemical actin-activated myosin ATPase cycle are introduced.

第一章主要介绍肌节的结构、肌球蛋白和肌动蛋白的分子结构、肌球蛋白工作循环中的机械化学偶联。

Twenty-seven differential proteins were tested by MALDI-TOF-MS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNA-binding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

RESULTS: A well-resolved and reproducible 2-DE pattern of PBMC was obtained from patients with HBV associated HCC or LC. For HCC, the mean number of protein spots was 1186±43, with an average matching rate of 91.6%, and for LC, the mean number of protein spots was 1013±41, with an average matching rate of 90.2%. Twenty-seven differential proteins were tested by MALDI-TOFMS, and 16 were identified. Of the 16 proteins, 13 were up-regulated in patients with HCC, including heat-shock protein 27, member RAS oncogene family (RAB14), actin, alpha 1 antitrypsin, RNAbinding protein regulatory subunit and so on. By contrast, the levels of proteins such as PDX6, HSPA8 and MnSOD were significantly reduced in HCC patients.

结果：得到了分辨率较高、重复性较好的HBV相关性HCC及LC患者PBMC双向凝胶电泳图谱，HBV相关性HCC及LC患者PBMC凝胶的平均蛋白质点数分别为1186±43及1013±41；组内的平均匹配率分别为91.6%，90.20%对27个差异蛋白质点进行了质谱分析，鉴定出16个蛋白质，在HBV相关性HCC患者PBMC中表达明显增强的有热休克蛋白质27、ras癌基因家族(RAB14)、肌动蛋白、α1-抗胰蛋白及RNA结合蛋白调节亚基等13个蛋白点；而PDX6、HSPA8及锰超氧化物歧化酶在HCC患者PBMC中低表达。

The histochemistry staining of cytokeratin, vimentin, alkaline phosphatase were positive and that of α-actin,Ⅷ Ag, fibronectin and desmine were negative.

细胞角蛋白、波形蛋白和碱性磷酸酶染色阳性，α-肌动蛋白、Ⅷ因子相关抗原、纤维连接蛋白和结蛋白染色阴性。

Cytokeratin, vimentin, alkaline phosphatase,α-actin,Ⅷ Ag, fibronectin and desmine were determined using the third generation cells. The ultrastructure were examinated by the transmitted and scanning electronic microscope.

取第三代细胞检测细胞角蛋白、波形蛋白、碱性磷酸酶、结蛋白、Ⅷ因子、α-肌动蛋白的表达，应用扫描电镜和透射电镜观察细胞的超微结构。

The myosin exhibited actin-activated MgATPase activity and could be recognized immunologically by a monoclonal antibody against the skeletal muscle myosin.

这种肌球蛋白具有肌动蛋白激活的MgATPase活性，能够被抗动物肌肉的肌球蛋白的单克隆抗体识别。

The motor molecule forms bipolar thick filaments that interdigitate with thinner actin filaments in the saromere, forming the basic contractile unit of muscle.

在所有的分子马达中，关于肌球蛋白的研究最多。肌球蛋白是肌肉收缩的关键成份，由它们形成的双极粗丝在肌节内同肌动蛋白细丝交叉对插，构成一个收缩单元。

Morphologically similar lesions from 26 patients (mean age, 62 y; range, 46 to 88 y; male/female ratio, 10/16) were retrieved from surgical pathology and consult files. Clinical and endoscopic data were obtained, and immunohistochemistry for S-100 protein, glial fibrillary acidic protein, neurofilament protein, epithelial membrane antigen, claudin-1, CD34, smooth muscle actin, and KIT was performed. The findings were compared with those in mucosal biopsies of 5 submucosal neurofibromas from NF1 patients.

我们从外科病理和档案库里调取了26例病变形态相似的病例（患者平均年龄62岁，范围：46至88岁，男女比例：10/16），得到了他们的临床及内窥镜资料，并给它们都做了S-100蛋白、胶质纤维酸性蛋白、神经丝蛋白、上皮膜抗原、闭合蛋白-1（claudin-1）、CD34、平滑肌肌动蛋白和KIT的免疫组化，所得的结果都与5例NF1患者粘膜下神经纤维瘤的粘膜活检的结果相比较。

The method has thyroid transcribe factor to specimen of 12 SHL tissue - antigen of 1(TTF-1), epithelial film , CK7, CK20, dash forward the albumen that touch bubble , undee albumen , flowing flesh actin , placental alkalescent phosphoric acid is enzymatic , be addicted to bead albumen, among them observation of 10 lens that make report.

方法对12例SHL组织标本进行甲状腺转录因子-1(TTF-1)、上皮膜抗原、CK7、CK20、突触泡蛋白、波形蛋白、平滑肌肌动蛋白、胎盘碱性磷酸酶、嗜铬粒蛋白、CD34的SP免疫组织化学法标记，其中10例做电镜观察。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失