肌动蛋白

There was a great reduction of volume of nucleus pulpous cells following loading, and most of the actin in nucleus pulpous cells assembled around its membrane.

体外培养的单层椎间盘细胞在受力状态下，肌动蛋白的表达减少，细胞的体积缩小，并且肌动蛋白在细胞内重排列。

In this thesis, we studied the effects of nuclear actin on gene transcription regulation, with a special focus on the relations between the nuclear actin and RNA polymerase II in concanavalin A-stimulated lymphocytes and HeLa cells.

本文以ConA激活的淋巴细胞和HeLa细胞为实验材料，从不同角度研究了细胞核肌动蛋白与RNA聚合酶Ⅱ相互作用，并对细胞核肌动蛋白参与RNA聚合酶Ⅱ的作用机制进行了探讨。

Sequence analysis reveals the identity rate of β-actin cDNA nucleotide sequence between the rice field eel and Tilapiamossambica, Salmo salar, Oryzias latipes, Oncorhynchus mykiss, Danio rerio, Carassius auratus, Physalaemus pustulosus, Bos taurus, Gall us gallus, Mus musculus, Hoino sapiens are 68%~95%; and the similarity of 3-actin amino acid sequence is 97%~100%.

将所得序列与各科鱼、蛙、鸡、牛、鼠、人等的β-肌动蛋白基因序列同源性分析显示，核苷酸序列具有68%~95%的相似性，氨基酸序列具有97%~100%相似性，显示该基因在生物进化过程中的保守；系统发育分析表明黄鳝β-肌动蛋白基因与罗非鱼的亲缘关系最近。

After developing 48 hours, hepatocytes enters 2.0 mM t-butyl hydroperxide or 2.0 mM diamide to induced cell blebbing. The samples collected at 0, 15, 30, 60 minutes to analyze cell about membrane protein thiol deletion; the leakage of lactate dehydrodenase; the change of glutathione; the lipid peroxidation; the variation of actin and tubulin, and use Confocal fluorescent microscope to observe the change of actin and tubulin in rat hepatocytes.

肝细胞在48小时培养后，分别加入2.0mM t-buty1 hydroperoxide或2.0 mM diamide诱发细胞膜小泡生成，并分别於0、15、30、60分钟取样，分析细胞膜硫醇流失程度；乳酸去氢□渗漏；□胱甘□浓度变化；脂质过氧化；肌动蛋白与管蛋白变化；并利用雷射共轭焦萤光显微镜观察肝细胞中肌动蛋白及管蛋白的变化。

Results: MC of tryptase immunoreactivity contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase temporarily was stored in secretory vacuole, and small amounts tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

Results: MC of tryptase immunoreactivity MC(subscript T contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase was temporarily stored in secretory vacuole, and small amounts of tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果：类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环，在质膜内层区域形成阻碍类胰蛋白酶释放的屏障；大量的类胰蛋白酶暂存于分泌泡中，少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

This data not noly suggest that polymerization-depolymerization is important to stomatal movement and stability, but also interpret conflicting results whether actin polymerization or depolymerization in stmatal guard cell.

这不仅提出了肌动蛋白解聚—聚合在气孔开关运动和稳定性中的重要作用，而且可以解释保卫细胞中肌动蛋白是处于解聚状态还是聚合状态的不同报道。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

To elucidate the function of actin in translation we have studied 25 isogenic conditional yeast actin mutants.

为了阐明肌动蛋白在翻译中的功能，我们研究了25个同基因，同条件的酵母肌动蛋白突变体。

Immunoaffinity purification of the 47kD actin-binding protein on a MoAb1 matrix yielded a multimolecular complex composed of actin and two other proteins (MWapp 180kD, 71kD).

在MoAb1基质对47KD肌动蛋白结合蛋白亲和纯化，得出一个由肌动蛋白和其它二个分子量约为180KD及71KD结合的复合物。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。