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肌动蛋白

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Whereas, in unactivated mouse spleen T lymthocytes, no binding could be found. Actin was closely associated to BAF complex and RNA Polymerase Ⅱ, but not so closely to NF1/CTF.

肌动蛋白与BAF复合物和RNA聚合酶Ⅱ的联系紧密,与转录因子NF1/CTF的联系不太密切。

In this study we provide evidence that inhibition of Rho kinase activation or myosin contractility abrogates PMA-induced apoptosis, and turns cells to undergo PMA-induced differentiation program, suggesting a switching role for the RhoAJROCK/myosin contraction pathway in cell fate decision.

在我们的研究中发现抑制Rho kinase 的活性或肌动蛋白收缩将阻断凋亡的发生,而使细胞全数走向分化,证实了RhoA/RocK 活化所造成的myosin 滑动收缩的机制,对细胞因应刺激而决定其命运的过程中扮演一决定性角色。

A The slimmest single filament is most similar to F-actin in size with ~7 nm width at half height and ~35 nmpitch ; the filaments have much wider diameter than the slimmest F-actin ; The branch filament could be con-stituted of two enlaced slimmer filaments , forming the branch in the joining point of another filament ; Two fila-ments are aligned side by side , and thought that there might exist certain interaction between them , which could be theearly step for further assembly of giant filaments.

肌动蛋白可通过自组织过程聚合形成不同直径(7nm~35nm)的长纤维,实验中观察到的最细纤维的结构参数与单根无鬼笔环肽结合时的F-actin[19 ]一致(Fig。 2 a);此外,还有大量具有较大直径和分支结构的纤维出现,表明有不同于单根微丝和微丝束的高级纤维结构产生Fig。

PHBV was implanted subcutaneously in rabbit and extirpated at 2,4,6,8, and 10 weeks in order to study its biocompatibility and biodegradability.

体外培养犬主动脉瓣间质细胞,观察其生长曲线,平滑肌α肌动蛋白表达和透射电子显微镜下特点。

The full-length genome cDNA containing genetic tags and flanked by Hammerhead ribozyme and Hepatitis delta ribozyme were cloned downstream of the CMV enhancer and the chicken beta actin promoter of the vector pCAGGS. After purified by Plasmid Midi Kit, the recombinant infectious clones named pCAGGmGtAHRT and pCAGGmGtBHRT were directly co-transfected DFI cells indued by Lipofectamine~ 2000. 72h post transfection. the supernatant of cell cuture was harvested and continue passaged in CEF cell. The results of indirect immunofluorescence.

分别在Gt株基因组两端引入了锤头状核酶结构(Hammerhead ribozyme,HamRz)和丁肝病毒核酶结构(Hepatitis delta ribozyme,HdvRz),并将其克隆在真核表达载体pCAGGS的CMV增强子和β肌动蛋白启动子下游,构建IBDV感染性克隆pCAGGmGtAHRT和pCAGGmGtBHRT,经Plasmid Midi Kit纯化后,在Lipofectamine~ 2000的介导下,直接转染DFI细胞,72h后取上清继续在CEF上传代。

On the sixtieth postoperative day, the graft was harvested and the blood was taken from inferior cava venae. Histological changes were monitored by HE stain. The infiltration of CD4+ and CD8+ T lymphocytes and the expression ofα- smooth muscle actin were detected by immunohistochemistry.

术后60天取移植血管做病理组织学检查并利用计算机图象分析系统测量血管内膜厚度;免疫组织化学检测CD4+、CD8+T淋巴细胞的浸润及α-平滑肌肌动蛋白的表达;ELISA法检测血清中血管内皮生长因子的变化。

The digestions should be less than 6 times, and every digestion should last for less than 20 min. Two-hour differential adherence could improve the purity of myocardial cells. By this protocol, 94.6% of cultured cells were live myocardial cells.

分离细胞时消化次数最好控制在不多于6次,每次消化时间不超过20min.2h差速贴壁可以提高心肌细胞纯度,经肌动蛋白鉴定,心肌细胞达94.6%。

Actin, as a component of the complex skeleton in the eucaryotic cell, takes part on many important cellular functional progresses.

肌动蛋白是真核细胞骨架的主要成分,与细胞内的许多重要功能活动有关。

Actin is a kind of highly conserved protein,existing in all eukaryote.

肌动蛋白是一种高度保守的蛋白质,存在于所有真核细胞中,参与细胞分裂、运动、迁移、形态的维持、生长等多种重要生理活动。

Make a copyof rat pulmonary fibrosis by infusing bleomycin through trachea , isolate and the pulmonary fibroblast, then interfered by atorvastatin ,divided into 3 groups of vacant ,TGF-β1 induced and atorvastatin interceped of different dosage , observe the structure of the cell by fiberscope ,the multiplication of the cell by MTT counting method, the expression of theα-SMA by immunity tissue chemistry staining method .

气管内注入博莱霉素(bleomycin,BLM)复制大鼠肺纤维化模型,并分离培养肺成纤维细胞,用阿托伐他汀对其进行干预,分为空白组、TGF-β1诱导组和不同剂量阿托伐他汀阻断组,用倒置纤维镜观察细胞的形态结构,MTT计数法观察各组细胞的增殖情况,免疫组化染色法定性观察α-平滑肌肌动蛋白(α-smooth muscleactin ,α-SMA)的表达情况。

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