肌动蛋白

Methods Salley′s leukoplakia carcinogenesis model of golden hamster cheek pouch is used in this study. The results are analyzed correlatively among pathological chassification, prepared-chinese-ink injection and α-smooth muscle actin immunohistochemistry.

材料与方法采用Salley法诱导金地鼠颊囊白斑癌变，以光镜下病理观察诊断分级和墨汁灌注、甲平滑肌肌动蛋白免疫组化等与血管生成有关的检测结果作相关性分析。

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 beta？？？？

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits. Each value was normalized against that of β actin mRNA.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 mRNA水平的比值。

OBJECTIVE: To study the relationship between intracellular actin and scar contracture.

目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白与瘢痕挛缩的关系。

Objective To study the relationship between intra cellular actin and scar contracture.

目的 探讨增生性瘢痕、瘢痕疙瘩成纤维细胞中肌动蛋白与瘢痕挛缩的关系。

Total RNA was isolated from the third instar larval body of the black cutworm, Agrotis ipsilon.The two actin cDNA sequences were cloned by RT-PCR and rapid amplification of cDNA ends and were designated as Aiactin1 and Aiactin2, respectively.

本研究以鳞翅目夜蛾科昆虫小地老虎［Agrotis ipsilon］3龄幼虫整个虫体为材料提取总RNA，利用RT-PCR和cDNA末端快速扩增技术，分别扩增得到2条肌动蛋白的cDNA序列Aiactin1和Aiactin2。

Actin is a highly conserved protein, which exists in almost all eukaryotic cells and has been proved to be essential for many cellular functions such as movement, morphology, growth, cytokinesis as well as other crucial events.

肌动蛋白是一类高度保守的蛋白质，存在于所有的真核细胞中，参与细胞分裂、运动、迁移、形态的维持、生长等许多重要生理活动。

Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

Summary: In this study, the change of adhensive struture, adhensive moleculd and actin filament in K-ras gene transfected pulmornary fibrocyte of mice, and the mechanism of invasion and metastasis of cancer cell were investigated by molecular biological techniques.

小结：本研究利用分子生物学技术，探讨K-ras基因导入的鼠肺纤维细胞—恶性转化细胞的粘附结构、粘附分子和肌动蛋白的改变，旨在探讨肿瘤细胞的浸润转移机制。

Morrison told the Jamaica Gleaner newspaper that research conducted with the University of Glasgow has identified a special component, called Actinen A, found in the "fast twitch fibres" in muscles.

莫里森告诉牙买加格宁那报说，一项由格拉斯哥大学实施的研究已经证明在肌肉的"快速启动纤维"有一种称为肌动蛋白 A 的特殊成份。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。