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Objective:Microfilament is one component of cytoskeleton , and it is mainly composed of actin.

背景和目的:微丝是细胞骨架的成分之一,主要由肌动蛋白组成。

Actin cytoskeleton is the major player in the cell motility and locomotion in most eukaryotic cells.

肌动蛋白组成的微丝骨架是大多数运动细胞的主要成分。

This cell is in anaphase of mitosis. Microtubules are shown in green, actin is in red and mitotic chromosomes are colored blue.

绿色的是微丝,红色为肌动蛋白,正在有丝分裂的染色体为蓝色,正在向两极运动。

METHODS: These patela were fixed and tested in the measure rack using electrometric technique under 50, 100, 150, 200, 250, and 300 N load. The anterior patella stress without fixation, and following stainless steel wire fixation or Ni-Ti Alloy wire fixation was measured.

股四头肌拉力以50,100,150,200,250,300 N加载和卸载两种方式作用于受试髌骨,应用电测法测量不完全横形髌骨骨折未经金属丝固定、钢丝环形加"8"字内固定及镍钛合金丝环形加"8"字内固定后的髌骨前面应力。

They are involed of intracellular movements in plant and animal cells.

细胞骨架的大部分主要是由可收缩的蛋白肌动蛋白组成的细长的微丝。

A large portion of the cytoskeleton consists of threadlike microfilaments composed mainly of thecontractile protein actin.

细胞骨架大部分由微丝组成,微丝主要由可收缩的肌动蛋白组成。

A The slimmest single filament is most similar to F-actin in size with ~7 nm width at half height and ~35 nmpitch ; the filaments have much wider diameter than the slimmest F-actin ; The branch filament could be con-stituted of two enlaced slimmer filaments , forming the branch in the joining point of another filament ; Two fila-ments are aligned side by side , and thought that there might exist certain interaction between them , which could be theearly step for further assembly of giant filaments.

肌动蛋白可通过自组织过程聚合形成不同直径(7nm~35nm)的长纤维,实验中观察到的最细纤维的结构参数与单根无鬼笔环肽结合时的F-actin[19 ]一致(Fig。 2 a);此外,还有大量具有较大直径和分支结构的纤维出现,表明有不同于单根微丝和微丝束的高级纤维结构产生Fig。

Actin filaments can form long and thin bundles in the filopodia, and can also exist as the actin meshwork in the lamelipodia.

肌球蛋白通常与肌动蛋白结合成束形成张力纤维丝。

Lamblia were cultivated axenically with modified TYI-S-33 medium contained dihydroartemisinin (0.002 mg/L) for 12 h and then stained by rhodanmine phalloidin and paclitaxel (P22310). The cytoskeletons of the organisms were detected and analyzed by flow cytometry, and then observed by confocal microscopy.RESULTS: The cytoskeleton of trophozoites was markedly damaged after treatment with 0.002 mg/L dihydroartemisinin for 12 h.

将用含双氢青蒿素的改良TYI-S-33培养基培养后的蓝氏贾第鞭毛虫滋养体,用专一性结合f-肌动蛋白的荧光染料罗丹明-鬼笔环肽(rhodanmine phalloidin, RDP)标记微丝,结合微管的紫杉醇(Paclitaxel, Oregen Green 488, P22310)标记微管,用流式细胞术检测、分析,用激光共聚焦显微镜(confocal microscopy, CFM)观察滋养体微丝和微管的变化。

YAO Jun-Feng, ZHANG Ying, WU Gui-Qin, ZHENG Jiang-Xia, DENG Xue-Mei, YANG NingCollege of Animal Science and Technology, China Agricultural University, Beijing 100094, ChinaSeven single nucleotide polymorphisms were identified by PCR-SSCP and sequencing in the chicken apoA5 gene in F2 chickens from an experimental cross of White Plymouth Rock × Silkies.

以丝羽乌骨鸡和隐性白洛克正反交产生的F2代为实验群体,采用PCR-SSCP和DNA测序的方法检测鸡载脂蛋白A5(apoA5)基因的单核苷酸多态性,并将所发现的SNPs与体重、胸肌重、腿肌重、心脏重、肝脏重和腹脂重等屠体性状进行关联分析。

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