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Results: MC of tryptase immunoreactivity contain abundant F-actin ring that region of cytomembrane endothecium form a barrier of blocking tryptase liberation. Copious tryptase temporarily was stored in secretory vacuole, and small amounts tryptase was released from secretory vacuole for depolymerize of F-actin ring in cell.

结果:类胰蛋白酶免疫反应性的肥大细胞内含有丰富的丝状肌动蛋白环,在质膜内层区域形成阻碍类胰蛋白酶释放的屏障;大量的类胰蛋白酶暂存于分泌泡中,少量的类胰蛋白酶因细胞内丝状肌动蛋白环的解聚使之从分泌泡中释放。

Result: At two weeks after OVA challenge, in model group airway wall thickness and positive cell nucleus of airway smooth muscle begin to grow up. At four weeks after challenge, airway smooth muscle content begins to elevate. At two and four weeks, expression of phenotype mark protein begins to low down, except for sm α-actin at two weeks.

结果发现,模型组大鼠激发二周后出现管壁增厚,四周后出现平滑肌层增厚,二周时即开始出现气道平滑肌Brdu掺入增多;smα-actin于二周时表达增强,而于四周时减弱,sm-MHC、calponin在二周、四周时表达均出现减少;电镜发现平滑肌细胞肌丝稍减少,而高尔基体、粗面内质网等细胞器增多。

Both the new peniculus 1 and the old haplokinety separated at the telophase.The two germinal rows germinal rowsfor both the daughter cells appeared almost at the same time;(2) The macronucleus became shorter and thicker, finally oblate during the division of the cell. Then the macronucleus restored its original shape, band-like form. The micronucleus were divided into two before the macronucleus division;(3) The original scopula and stalk contributed to the old cell. The new cell formed scopula gradually after the cell division.

同时,新仔虫的第一咽膜(P′1)也开始由老单毛基索复制,并在细胞分裂后期与老结构分离;(2)大核在虫体分裂过程中由长带状逐渐缩短变粗至扁圆形,于虫体即将分开时迅速拉长,然后分裂为二个新大核;小核分裂先于大核,在两仔虫口纤毛器即将分开时完成;(3)原帚胚及柄归属老仔虫,新仔虫的帚胚在虫体分裂后逐渐形成,柄内肌丝则在柄鞘形成后逐渐长出。

These results are consistent with the hypothesis that, following fiber activation, a significant portion of the optically anisotropic elements rotate towards the actin filament causing the interfilament mass content to undergo redistribution.

上述结果表明肌动蛋白丝和肌球蛋白丝之间的重叠区和非重叠区中横桥的平均取向是不同的,在钙离子作用活化收缩时,会导致肌动蛋白丝和肌球蛋白丝之间的质量重新分配。

In addition, SMC transfected with aM2 commo nly appeared bunches of myofilament in ultramicroscopy, while aM1 and aM3 emerge d significant cellular degeneration.

另外,在转染了aM2 的SMC的超微结构中普遍出现明显的肌丝束,而aM1和aM3组则呈现明显的细胞退行性变。

Most endothelial cells fell off, elastic fibre autolyze; the number of SMC decreased, some SMC nucleus contracted completely or depressed, typical apoptosis body were seen in later stage, muscle silk autolyzed and disappeared mostly; in outer layer saw much collagenic fiber, arranged unorderly; inflammation cells were seen in the aneurysm wall(hypertrophy cell et al.).

多数瘤壁的内皮细胞溶解脱落,不连续,弹力纤维自溶;动脉瘤血管壁均可见SMC明显减少,较多SMC染色质边聚、固缩,部分SMC核全部固缩或出现不规则凹陷,SMC核胞浆或胞核部分出现脱落或裂解成碎片,晚期可见典型的凋亡小体出现,肌丝大都自溶消失:外膜可见大量的胶原纤维,排列较紊乱;瘤壁还可见炎性细胞浸润。

Under fluorescence microscope, cytolymph could emit red fluorescence with Rhodamine, and cellular nucleus could emit blue fluorescence with DAPI, accompanying myofilament structure.

免疫细胞化学检测:荧光显微镜下,罗丹明染色细胞浆呈红色荧光,并可见肌丝结构;DAPI染色细胞核呈蓝色荧光。

It was showed that pectenolone and pectenoxanthin were all found in Gonads, hepatopancreas, mantle and gill, among of them, ovary, hepatopancreas and gill possessed more carotenoid than other tissues, but no carotenoids were found in the white muscles of Yesso scallops. Hepatopancreas contains a variety other carotenoids some kind of other not identified beside pectenolone and pectenoxanthin. The result also showed that the Yesso scallop with orange muscle had more the carotenoids in a variety of tissues than the common, and the female more than the male.4 Impacts of carotenoid accumulation on morphological structure and chemical composition in orange muscle of Yesso scallopThere was no difference between the orange and white muscle obserced by conventional HE staining, while musclular fiber type and matrix density in the orange muscle was different from white ones by the TEM observation of ultra-thin slices, and there were three musclular fiber types, among of them, the loose muscular fiber was orange muscle specifically.

利用HPLC法对橘红/白色闭壳肌虾夷扇贝各组织中含有的类胡萝卜素种类和含量,结果表明,虾夷扇贝在性腺、肝胰腺、外套膜、鳃丝中均含有类胡萝卜素pectenolone和pectenoxanthin,白色闭壳肌中未检测到类胡萝卜素,其中肝胰腺除上述两种类胡萝卜素外,还含有其他多种未鉴定的类胡萝卜素;在各组织中,雌性性腺、肝胰腺、鳃丝中类胡萝卜素的含量较高;橘红色闭壳肌虾夷扇贝各组织中的类胡萝卜素含量高于白色闭壳肌虾夷扇贝;雌性虾夷扇贝中的类胡萝卜素的含量高于雄性。4累积类胡萝卜素对虾夷扇贝闭壳肌的形态结构和化学成分的影响本文对橘红色闭壳肌进行了常规HE染色切片观察,并未观察到异常现象;超薄切片透射电镜观察发现橘红色闭壳肌在肌纤维类型及基质密度等方面有别于白色闭壳肌,在橘红色闭壳肌中有三种肌纤维,其中疏松型肌纤维为橘红色闭壳肌特有。

The myocardial fibers are beginning to lose cross striations and the nuclei are not clearly visible in most of the cells seen here.

心肌纤维的肌丝肌节结构破坏并且大多数细胞核不清楚,注意许多不规则的较暗的粉红的波浪状的并列的肌纤维。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞,CM联合20%FBS诱导骨髓间充质干细胞,同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组,分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化,并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化,用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

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