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Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化;RT-PCR和Western blotting检测表型标志分子α-动蛋白、球蛋白重链(SM1/2)、动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化;抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达,包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases, ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor, Rho-GDI alpha),研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

The findings indicate that the training method of trunk flexors and trunk extensors combined trunk rotators is more effective in stroke patients.

依据脑卒中患者躯干力低下的特点,选择性地强化对部分躯干的训练,即在加强前屈和后伸群训练的同时,增加躯干旋转群的训练,可以更加有效地提高对脑卒中患者平衡功能障碍的训练效果。

A transmiting electron microscope study was conducted of RWW to illustrate the ultrastructural changes during flight muscle development and degeneration.

为了解稻水象甲飞行发育和消解过程中飞行超微结构的变化,本文用透射电镜对其飞行进行了超微结构的研究。

The ratio of protein concentration of the A and I bands in the myofibrils of the glycerine extracted indirect flight muscle of the honey bee stretched to different degrees has been measured with the aid of the Baker interference microscope.

本文报告伸展程度不同的蜜蜂間接飞翔原纤維A带和I带蛋白浓度的比例。当小节长度从3.0微米增加到4.7微米,蛋白浓度之比稍稍上升。

The sensitive nymph instar and sexual differences of wing dimorphism in the brown planthopper, Nilaparvata lugens , were studied, and the developmental differentiations of both the indirect flight muscles and the wing buds in the brown planthopper were observed. Molecular biological studies were performed to amplify the indirect flight muscle specific actin gene.

本文研究了褐飞虱Nilaparvata lugens翅型分化的若虫敏感临界龄期和翅型分化在性别上的差异,观察了褐飞虱间接飞行和翅芽发育的分化过程,在此基础上,以扩增、克隆褐飞虱间接飞行特异性动蛋白基因为目的,做了部分分子生物学工作。

Methods BTXA labeled with125Iwas obtained. The radioactivitymeasurementwas taken tomeasure the solubility ofBTXA in gel and BTXA solution. The rightgastrocnemiusmuscles of12 SD ratswere injectedwithBTXA geland the leftgastrocnemiusmuscles injectedwith normal salinewere used as controls. HE staining and 1% gold chloride stainingwere used to study the tissue changes 6 months and 12 months afterBTXA gel injection and transmission electronmicroscopy was used to observe the ultrastructural changes.

将BTXA行示踪标记配制125I-BTXA水凝胶与125I-BTXA水溶液,进行样品放射性强度测定。12只SD大鼠用随机数字表法分为A、B两组,每组6只,大鼠右侧腓肠注射BTXA水凝胶,左侧腓肠注射等体积生理盐水作对照,于注射后6个月、12个月切取腓肠标本,常规病理HE染色、1%氯化金染色,透射电子显微镜观察组织结构变化。

The musculocutaneous nerve was always the studied target in the experimental animal model of the root avulsion of the brachial plexus. The C〓 root was the main component of musculocutaneous nerve, the transection of C〓 was used in the experimental study of multi-nerve transfer before.

模拟臂丛神经根性撕脱伤的动物模型通常都以皮神经作为研究对象,除了其终末支可用于神经移位以外,由于皮神经以颈〓成分为主,故在颈部切断颈〓即为皮神经主干,可用于多组神经移位的研究。

In control group, obturator nerve controlling right gracilis was cut off. In in situ implantation group, the right gracilis was cut off and replanted to its original site, and the obturator nerve was implanted to the muscle. In ectopic transplantation group, the right gracilis was cut off and transplanted to the muscle of the left leg, and the obturator nerve was implanted to the muscle.

对照组:切断支配右侧股薄的闭孔神经,造成肉失神经支配;原位再植组:切取右侧股薄,再回植于原位,闭孔神经植入肉;异位移植寄养组:切取右侧股薄,移植寄养于左侧股部,闭孔神经植入肉。

Tube through the fibula and between flexor long, from the soleus muscle and between the peroneus longus muscle and out.

管穿过腓骨和长屈之间,从比目鱼和腓骨长之间穿出。

Results Group C had the symptoms such as reduced activity, acceded, indulge in lying and weight loss after 3 weeks of immune injection, 14 out of 16 SD rats in Group B had the same symptoms as Group C after 4~5 weeks of immune injection, the serum enzymes in model groups increased significantly compared with those of the control group, model group C was much higher than model group B; the duration shorted, amplitude decreased, multiphase wave increased in electromyogram of model groups; MRI examination revealed samples from model group B and C had one positive case each, which presented T1MI isodensity or hypodensity signal, T2MI and STIR serial hyperdensity signal, revealing muscle inflammation; all rats'skeletal muscle from model group C and 11 out of model group B had pathological changes, which exhibited striated muscle focal fiber degeneration, necrotized and inflammatory cell infiltration, interstitial vessel wall thickening, random cardiac muscle samples had 3 positive changes, which had similar changes to skeletal muscle, there was 1 positive change from lung sample.

结果:模型C组于免疫注射第3周左右开始出现活动减少,倦怠嗜卧,食欲体重下降等表现,模型B组有14只SD大鼠于免疫注射第4~5周出现上述症状,较C组为轻;模型组酶谱中,酸激酶、乳酸脱氢酶、谷草转氨酶与对照组比较明显升高,模型C组较模型B组升高更显著;模型组电图时限缩短,波幅降低,多相波增多;磁共振检查模型B组和C组选送标本中各有一例有阳性改变,表现为T1MI等或稍低信号,T2MI及STIR序列为高信号,提示肉炎症水肿改变;模型B组有11只,模型C组全部大鼠骨骼出现病理改变,表现为横纹局灶性分布的纤维变性炎细胞浸润,间质小血管壁增厚、扩张,随机选送的心标本中有3例有阳性改变,表现与骨骼相仿,选送的肺标本中有1例有阳性改变,表现为蛋白渗出,炎症细胞浸润和小血管改变。

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