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To systematicallyinvestigate this landmark, we conducted a study in four phases:1 Two blindfolded examiners determined the distance betweenthe tuberosity's medial border and the clavicle's lateral endin 100 dried clavicles and then 2 performed subclavian veincannulation in 20 fresh human cadavers using the tuberosityand the suprasternal notch as landmarks. 3 Three-dimensionalreconstructions of the subclavian artery and vein and surroundingstructures were derived from computed tomography datasets of10 patients. The length of the path of a virtual subclavianvein cannulation with the deltoid tuberosity landmark was measuredbilaterally. 4 In a prospective, randomized trial, subclavianvein cannulation was performed in 60 patients with a standardapproach or with the deltoid tuberosity as landmark.

为系统地研究这个解剖标志,作者将本研究分为四个阶段:1 双盲检查100个干燥的锁骨测量其三角粗隆内侧缘至锁骨侧面末端的距离;2 在20具新鲜的尸体上以三角粗隆和胸骨上切迹为标志进行锁骨下静脉穿刺;3 利用 CT 所获取的数据资料对10个病人的锁骨下动脉和静脉以及周围结构进行三维重建,测量两侧以三角粗隆为标志的虚拟锁骨下静脉置管路径的长度;4 选择60例病人以标准路径或三角粗隆为标志进行锁骨下静脉穿刺的前瞻性随机试验研究。

To systematicallyinvestigate this landmark, we conducted a study in four phases:1 Two blindfolded examiners determined the distance betweenthe tuberosity's medial border and the clavicle's lateral endin 100 dried clavicles and then 2 performed subclavian veincannulation in 20 fresh human cadavers using the tuberosityand the suprasternal notch as landmarks. 3 Three-dimensionalreconstructions of the subclavian artery and vein and surroundingstructures were derived from computed tomography datasets of10 patients. The length of the path of a virtual subclavianvein cannulation with the deltoid tuberosity landmark was measuredbilaterally. 4 In a prospective, randomized trial, subclavianvein cannulation was performed in 60 patients with a standardapproach or with the deltoid tuberosity as landmark.

为系统地研究这个解剖标志,作者将本研究分为四个阶段:1 双盲检查100个乾燥的锁骨测量其三角粗隆内侧缘至锁骨侧面末端的距离;2 在20具新鲜的尸体上以三角粗隆和胸骨上切迹为标志进行锁骨下静脉穿刺;3 利用 CT 所获取的资料资料对10个病人的锁骨下动脉和静脉以及周围结构进行三维重建,测量两侧以三角粗隆为标志的虚拟锁骨下静脉置管路径的长度;4 选择60例病人以标准路径或三角粗隆为标志进行锁骨下静脉穿刺的前瞻性随机试验研究。

Results: All 8 patients with INVM showed prominent excessive myocardial trabeculation and deep intrabecular. Endocardium of 25 patients with IDCM were smooth, except 2 patients, in which one had excessive myocardial trabeculations in beast apex, the other in lateral wall.

结果:8例INVM均有粗大的小梁和小梁间深陷的陷窝,25例IDCM 23例均未见粗大小梁及陷窝,1例在心尖部见轻度增粗的小梁,另1例在左室侧壁见数目有限的增粗小梁,并见其与假腱索相连。

This is the first report of pectenolone and pectenoxanthin, two types of carotenoid, in the muscle of the Yesso scallop.3 Roles of the carotenoids composition and accumulation in Yesso scallopThe method for detecting carotenoids using reverse phase high performance liquid chromatography was developed. The conditions of RP-HPLC analysis were an analytical scale (200×4.6 mm, inner diameter 5μm) Eclipse XDB-C18 column of Agilent corporation; 10μl injection volume; 25°C column temperature; acetonitrile, methanol and methylene dichloride mobile phase (40:56:4, V/VV) with 0.1% BHT; 1.0 ml per minute flow rate; 450 nm UV-VIS detector; 8 minutes elution.The carotenoids from orange muscle of Yesso scallop of 7, 10, 12, 15, 18, 22 months old was detected using the method refered above.

通过柱层析、高效液相等手段对橘红色闭壳中的主要类胡萝卜素进行了提取分离,分离提纯得到两种类胡萝卜素,并通过MS、~1H-NMR和~(13)C-NMR对其进行结构鉴定,结果表明(3S,3\'R)-3,3\'-dihydroxy-7\',8\'-didehydro-β,β-Caroten-4-one和(3S,3\'S)-7,7\',8,8\'-tetradehydro-β,β-Carotene-3,3\'-diol,俗称pectenolone和pectenoxanthin是橘红色闭壳中的主要类胡萝卜素,这是国内外首次对虾夷扇贝闭壳中的类胡萝卜素进行提纯及结构鉴定。3虾夷扇贝类胡萝卜素的种类组成和累积变化规律建立了橘红色闭壳中类胡萝卜素的HPLC分析定量检测技术:色普柱为Agilent公司的Eclipse XDB-C18柱200mm×4.6mm,i.d.5μm进样体积,5μl ;流速1ml/min;检测波长450nm;柱温25℃;流动相为乙腈:甲醇:二氯甲烷(V/V/V=40:56:4并加入0.1%的BHT;分离时间为8min。

Objective: To compare the effects of different doses of astragalus membranaceus injection on fatigue in nerve centre,neuromuscular junction and muscle in hoptoads and mice.

目的:利用已建立的蟾蜍和小鼠中枢、神经-接头和疲劳模型,比较不同剂量的黄芪注射液对蟾蜍和小鼠中枢、神经-接头和疲劳的影响。

According to the surface electromyogram to statistically analyse IEMG and knee joint in different phases, we can find the relationship among the main work muscle in swinging of hurdle step, the cooperative muscle of crura muscle in doing act, the change of angle in pressing and touching down, and skill action.

根据表面电图,对IEMG及不同阶段膝关节角度进行统计分析。结果发现,在跨栏步摆动腿动作中的主要工作,在完成相应动作的肉发力顺序,在完成动作过程中下肢肉表现出来的协作和对抗以及蹬地和着地时角度变化跟技术动作的关系。

Furthermore, it was noticed that there was clearly higher myofilaments density in superficial and intermediate lamellae than the deep one.

电镜观察进一步验证了酶组化的结果,并首次发现浅层和中间细胞丝密度明显大于深层

The myoblasts are the precursor of the maturated muscle cells, while the satellite cells are a kind of mononucleus cells which have the characteristics and function of the myoblasts. The satellite cells participate in the growth of skeletal muscle, the repair after injury of the muscle and provide nuclei to the maturated muscle for the normal alternation of cell nuclei. They are very important to the maintenance of the function of skeletal muscle.

细胞为成熟细胞的前体细胞,而卫星细胞则是成熟骨骼组织中具有成细胞特征和功能的一类细胞,参与骨骼的生长、损伤后的修复以及为正常骨骼细胞核的更替提供细胞核,对骨骼正常功能状态的维持很重要。

Plenty and purified satellite cells are obtained with these methods.IGF-1 could promote the proliferation and differentiation of mouse skeletal muscle satellite cell. Adequate single satellite cell could be obtained by frequent passaging method and the preparation are ready for for satellite cell transplanting.

结果:此方法可以获得足量、纯度较高的卫星细胞,适宜浓度的IGF对卫星细胞有较强的促增殖及促分化作用,频繁传代可以获取足量的单个卫星细胞,为卫星细胞移植做好了前期准备。

There were no limp,ulceration and phenomena of self-injury.Normal wave figure in EMG was noted in the experimental side. There was no significant difference in t test in EMG wave amplitude,maximum tetanized systole tensile strength and duration of isometric contraction.

结果 术后大鼠两侧姿态对称,未见跛行,无溃疡及自残现象,对疼痛刺激敏感;实验侧肱二头和指深屈电图均呈正常波形;两侧电图波幅、等长收缩时最大强直收缩张力及持续时间进行自身t检验比较均无显著差异。

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