肌

EMG signal analysis software from DASYLab 6.0 rectus femoralis, biceps femoris, tibialis anterior , the gastrocnemius and other muscles of the original EMG signal, the 10-500Hz to the band-pass filter processing, the full-wave rectifier on the turn, rate of 10 Hz low-pass smoothing wave treatment, and then by the integral operation, the available IEMG values, and divided by the integral interval of time, come to the average EMG EMG signal amplitude, and action cycle the largest standardized EMG values.

肌电讯号由DASYLab 6.0软体分析股直肌、股二头肌、胫骨前肌、腓肠肌等肌群之原始肌电讯号后，进行10-500Hz的band-pass滤波处理，经全波整流上翻、10 Hz低通率波平滑化处理，再经积分运算后，可得积分肌电值，并除以积分区间之作用时间，得出肌电讯号的平均肌电振幅，并以动作周期中最大肌电值进行标准化处理。

Results The anatomic structures of "Jianzhongshu" for perpendicular insertion downward were skin, superficial fascia, trapezius, rhomboideus, splenius cervicis, erector spinae, suprapleural membrane and cupula of pleura in order.The anatomic structures of "Dazhu" were skin, superficial fascia, trapezius, rhomboideus, upper posterior serratus, splenius cervicis, erector spinae, levator ribs, internal intercostal membrane and costal pleura in order.

结果 肩中俞向下直刺的解剖结构依次是皮肤、浅筋膜、斜方肌、菱形肌、颈夹肌、竖脊肌、胸膜上膜、胸膜顶；大杼穴向下直刺的解剖结构依次是皮肤、浅筋膜、斜方肌、菱形肌、上后锯肌、颈夹肌、竖脊肌、肋提肌、肋间内膜、肋胸膜。

We also observed and measured the distance and section of some muscles such as long muscle of neck、long muscle of head、sternocleidomastoid、trapezius、longissimus、inultifidus、splenius、semispinal muscle of head、semispinal muscle of neck、anterior scalene、middle scalene 、posterior scalene et al. We achieved the moment of force and downloaded the force in the model .We also added gravity of head.

通过横截面积肌力计算法，测量尸体颈椎周围的主要肌肉：胸锁乳突肌、斜方肌、头长肌、颈长肌、肩胛提肌、夹肌、前斜角肌、中斜角肌、后斜角肌、多裂肌等的横截面积及其与颈椎纵轴的中心距数值，求得力矩值，直接或根据力矩等价公式间接的将肌力值加载于模型体表各点。

Purification of mlckfrom rabbit skeletal muscle 肌球蛋白轻链激酶...used by:Stull Lab,University of Texas, Southwestern Medical Center

肌球蛋白轻链激酶( myosin light chain kinase，mlck )具有激酶活性和非激酶活性，在平滑肌收缩过程中起着关键酶调控的作用。

The unoperated sides of the treated animals also served as controls. Six normal rats were treated as normal control group. Three different siRNA plasmid solution containing RC2-Ⅰ, MAFbx-Ⅱ, CON (50μl , 0.8μg/μl)was injected and transfected by electroporation as methods mentioned above, respectively. The changes of RC2 and MAFbx mRNA levels and RC2 protein levels after 3 days were determined by real-time quantitative PCR and Western blot, respectively. On postoperative 2, 3 and 4 weeks, the rate of wet muscle weight preservation, mean diameter of muscle fiber and mean cross-section area of muscle fiber and muscle protein content were checked and then compared between group CON and group RC2 or group MAFbx, respectively. The differences between groups were analyzed by one-way ANOVA. Ultrastructural changes of muscle fiber were observed at 2, 3, 4 weeks postoperation.Results GFP plasmid was efficiently deliverd into muscle by electroporation and robust GFP expression in muscle could be observed more than three weeks. Histology shows that injected plasmid DNA diffuses extensively in muscle tissue.

1、健康雌性SD大鼠18只，随机分为电穿孔组和非电穿孔组，每组9只，制作右下肢趾长伸肌失神经支配模型；EP组为将质粒pEGFP-N1溶液50μl(0.8μg／μl)注射入右趾长伸肌后，立即于两侧腱腹交接处给予电穿孔，电穿孔参数为：电场强度为200V／Cm，脉冲100μs，频率1Hz，施加10次脉冲；NEP组仅质粒pEGFP-N1溶液注射；转染后1、2、3周，荧光显微镜下观察趾长伸肌中GFP的表达情况，转染后1周行Western印迹检测趾长伸肌中GFP蛋白的表达情况，检测和优化体内转染效率。2、健康雌性SD大鼠78只，随机分为失神经对照组、RC2基因治疗组(RC2组)，MAFbx基因治疗组，每组24只，制作右下肢趾长伸肌失神经支配模型，余6只为正常组；分别将含CON、RC2、MAFbx基因的siRNA重组质粒注射入趾长伸肌，之后给予电穿孔，方法同上；治疗后3天实时定量PCR和Western印迹检测各组中RC2或MAFbx基因的mRNA和蛋白的表达变化，治疗后2、3、4周检测各组肌湿重维持率、肌细胞直径和肌细胞截面积，肌细胞超微结构变化以及肌纤维中蛋白含量变化。

The results are as follows.(1) Long train electrical stimulation at the dMNF facilitated myoelectric activities of genioglossus and phrenic muscle.(2) Short train electrical stimulation of dMNF, when delivered in the inspiratory phase, caused prolongation of myoelectric activities of genioglossus and phrenic muscle; but when delivered in the expiratory phase, myoelectric activities of genioglossus and phrenic muscle were shortened.(3) Long train electrical stimulation at the vMNF inhibited myoelectric activities of genioglossus and phrenic muscle.(4) Short train electrical stimulation of vMNF caused shortening of myoelectric activities of genioglossus and phrenic muscle, when delivered in the inspiratory phase, but a prolongation when delivered in the expiratory phase.(5) The effects of microinjection of glutamate to dMNF and vMNF were similar to those of electrical stimulation.

结果如下：(1)长串电脉冲刺激dMNF引起颏舌肌和膈肌肌电活动明显增强；(2)短串电脉冲刺激dMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动延长，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动提前终止；(3)长串电脉冲刺激vMNF使颏舌肌和膈肌肌电活动明显被抑制；(4)短串电脉冲刺激vMNF，当刺激落位于吸气相时，引起颏舌肌和膈肌在吸气相的肌电活动提前终止，当刺激落位于呼气相时，引起颏舌肌和膈肌在呼气相的肌电活动延长；(5)在dMNF和vMNF分别微量注射谷氨酸钠，其效应与电刺激结果基本一致。

The results showed an increased mean jitter value, fiber density and abnormal percentage both in EDC and AT muscles in group 1 and II diabetic patients comparing to the normal controls. These findings suggest an impaired or immatured neuromuscular junctions and an evidence of reinnervation through axonal sprouting in the diabetic patients either with or without nerve conduction abnormalities. In conclusion, the changes of axonal degeneration and reinnervation are the main pathophysiological mechanism of diabetic neuropathy, and the SFEMG is more sensitive than routine nerve conduction study in the diagnosis of diabetic neuropathyKeyword Axonal degeneration , diabetes mellitus , neuropathy , single fiber electromyography

结果显示两组糖尿病病患的连续间值差异，肌纤维密度及不正常比率比对照组显著增加(t试验，依序p.01)，在第一组糖尿病患中，伸指总肌的连续波间值差异比第二组高(p.05)，而连续波间值差异与纤维密度之间也成线性相关的增加(伸指总肌，r=0.471，p.01；胫前肌，r=0.386，p.02)，在伸指总肌测得的连续波间值差异上，第一组糖尿病患有75%不正常，第二组有33.3%不正常，而在胫前肌的连续波间值差异上，第一组糖尿病患有83.3%的不正常，第二组有75%不正常，在肌纤维密度的测定中，第一组糖尿病患的伸指总肌有27.8%不正常，胫前肌有58.3%不正常，第二组病患的伸指总肌有25%不正常，胫前肌有41.7%不正常，此结果表示糖尿病神经变造成的神经肌像交接处不稳定及有神经轴再生的重分布现象，因此也证实糖尿病神经病变的主要病理机转为神经的退化所致，而在诊断上单纤维肌电图检查的敏感度也比运动神经传导速度检查为高。

The result showed that, the body wall most outer layer cuticle assumesthe translucent porodine, the surrounding entire body wall; Close isconnected with the cuticle 表皮层 for 合胞体 the structure, inthe back, the abdomen and its two sides separately adds thick to inprominent Cheng Jizhuang, separately contains the back side,腹侧the nerve and compares to the developed back, the abdomen nerve; Themyo- position to the body wall most inner layer, has the muscle cellto arrange becomes; Between the body wall and the digestive tractcavity is a body cavity; Digestive tract for 肌质 structure, afterinference for roundworm's esophagus.

结果显示，体壁最外层的角质层呈半透明的胶状，包围整个体壁；与角质层紧密相连的表皮层为为合胞体构造，在背、腹及其两个侧面分别加厚向内突出成脊状，分别包含背侧、腹侧神经和相比对发达的背、腹神经；肌层位于体壁最内层，有肌细胞排列而成；体壁与消化道之间的空腔为体腔；消化道为肌质结构，经推断为蛔虫的食道。

In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at 21st day before birth. With the development of rat digestive tract, neurokinin A-immunoreactivity positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexes, while mature nerve fibres could be seen at one month after birth. 2. In rat stomach, the positive reaction of NKA initially happened in the myenteric plexus at the 14th day of embryo, and then appeared on circular muscles, longitudinal muscles, submucosal, muscularis mucosae, lamina propria and epithelium. 30 days after birth, expression of NKA is same as seen in adaulthood. 3. In rat small intestinal, the NKA-IR could first be found in the myenteric plexus of the duodenum, jejunum and ileum at 14th , 15th and 17th day before birth respectively. Then the NKA-IR occurred in the longitudinal muscle layer, circular muscle layer, intestinal villus, intestinal gland, muscularis mucosae, submucosa, submucosal plexes, mucosa plexus and deep muscular plexus.

结果 1、在食管，于胚胎21天的粘膜肌层和环肌层内观察到神经激肽A免疫阳性膨体纤维，出生后，随幼鼠的生长发育，相继在上皮，粘膜下层，纵肌层、肌间丛、粘膜下丛有NKA-IR表达，30天时已和成年鼠相似；2、在胃，首先于胚胎14天的肌间丛出现NKA-IR的表达，随发育相继在环肌、纵肌，粘膜下层、粘膜肌、固有膜及上皮内出现NKA-IR的表达，30天时具备成年鼠的分布特征；3、在小肠，分别于胚胎14、15、17天的十二指肠、空肠和回肠的肌间丛处出现NKA-IR的表达，随发育相继出现在纵肌、环肌、绒毛、小肠腺周、粘膜肌、粘膜下层、粘膜下丛、粘膜丛、深肌丛。

Pecs (pectorals, chest muscles of guys)胸肌●Their perfect pecs splashed across magazines and billboards.

他们完美的胸肌在杂志和广告看板上到处可见。（刊登在杂志或是广告看板上的男性模特儿露出上身的胸肌，表示健美。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。