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AIM: To establish the methods for rat costochondral growth plate chondrocyte separation and culture and investigate their biological features, thereby provide experimental bases for studying the regulation of chondrocyte proliferation and differentiation.

目的:建立大鼠肋生长板软骨细胞分离、培养的方法,探讨其生物学特性,为软骨细胞增殖分化的调控和软骨组织工程修复的研究建立实验基础。

Methods: rat costochondral growth plate chondrocytes were isolated and cultured. growth kinetics of rgcs during serial passage was analyzed, and α sma, collagen typeⅱ and gag expressions of primary to 5th passage rgcs were detected by immunocytochemistry, western blot and alcian blue staining, respectively.

分离、培养大鼠肋生长板软骨细胞,对原代至第5代rgcs进行细胞生长动力学分析,阿尔新兰染色、免疫细胞化学法和蛋白质印迹分别检测蛋白多糖,α sma和ⅱ型胶原的表达。

The design features of FGD duct are as follows: large stiffness of flue gas duct, application of reinforced ribs and internally supported bridge structure, installation of leading plate, prevention of internal overarm support reso.

该烟气脱硫工程烟道的设计特点是:烟道的刚性较大;采用了加固肋及内撑桁架;设置了导流板;防止了内撑杆共振;考虑了支吊架及热膨胀的问题;烟道的焊接作了特殊要求;设置了人孔门和烟道疏水等。

The article uses the plane simplifying calculation method and the space finite element method to analyze the bearing characteristics of the arch rib when the concrete is poured into the steel pipe and the batten plate chamber of truss type steel pipe concrete tied arch bridge.

采用平面简化计算方法与空间有限元方法对桁架式钢管混凝土系杆拱桥灌注钢管及缀板腔内混凝土时拱肋的受力特性进行了分析。

Key words:big space; composite box; net beam ceiling; rib beam; board uniting with beam; polymerization cement slurry

关键词]大空间;叠合箱;网梁楼盖;肋梁;梁板合一;聚合水泥浆

The cold-formed sheet is a groove-shaped structure that is composed of a crown plate and reinforced ribs connected with ends of the crown plate; the box beam is a box-shaped structure comprising an upper wing edge, a lower wing edge and two side webs that are connected in every two components.

由压型钢板、纤维增强塑料箱梁组成,压型钢板为由顶板和顶板端部的加强肋相连组成的槽形结构,箱梁为由上翼缘、下翼缘和两侧腹板两两相连组成的箱形结构,其结构如图所示。

Besides these,deck beam finite element analysis method can be applied flexibly to box girders with different cross-section or rib-plank structure and those without orthogonal support .

板段元法适用于其他断面形状的箱梁结构或肋板式结构,也可用于斜交箱形直梁的分析,具有较好的适用性。

In cast-in-place concrete structure, high-strength cement concrete hollow tube technology is the common beam, floor slab of ribs, prestressed plate without bond after an in-situ development of the new structure of the new system.

在现浇砼结构中,高强水泥薄壁管空心砼技术是继普通梁板、密肋楼板,无粘结预应力平板后新开发的一种现浇新结构体系。

The result shows that the ultimate strength of the joint is related not only to the wall thickness t, diameter D, length of gusset plate B and the yield deflection at the top of the relevant section under the current status of the region, but also to the ribbed plate.

结果表明:极限承载力不仅与主管壁厚t、管径D、节点板长度B、现时状态下屈服区域内中截面顶部挠度δ有关,更重要的是还和现时状态下的加劲肋有密切相关。

I will set my face against that man, and his kindred, and will cut off both him and all that consented with him, to commit fornication with Moloch, out of the midst of their people.

我自己要板起脸来与这人和他的家族作对,将他和一切随著他敬拜摩肋客的人,由他们的人民中间一律铲除。

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