肉瘤

Calcium sulfate has been used for bone regenation in oral medicine. Osteopontin, a marker molecule, expressed by osteoblast, plays multiple roles in human body. However, the effect of calcium sulfate on osteopontin expressed in osteoblasts is unclear. In this study, U-2 osteosarcoma osteoblastic cells were cultured in 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate. There was no effect in the proliferation of cells, suggesting that calcium sulfate up to 10 μM is biocompatible. The cells and conditioned media were collected for analyzing mRNA and protein levels of osteopontin by reverse-transcription polymerase chain reaction and Western blot, respectively.

硫酸钙用於口腔医学中的骨头再生已经有多年历史，骨桥素是一种被骨母细胞分泌且在人体扮演重要多重角色的标的分子，然而，硫酸钙在骨母细胞分泌硫酸钙上的影响仍未明朗，在本实验之中，培养U-2骨肉瘤类骨母细胞(U-2 osteosarcoma osteoblastic cells)於0、0.5、1、10 μM的硫酸钙之中，在细胞数目的复制上，四种硫酸钙浓度并无影响，显示出U-2骨肉瘤类骨母细胞培养於10 μM的硫酸钙仍是生物相容性的。

After that, we detect cell DNA heteroploid and cell cycle transformation of human osteosarcoma tissue by using flow cytometry method; next, we amplification the exon2 and exon3 of 〓 gene in 36 cases human osteosarcoma tissue, and amplification products were performed the sepharose and polyacrylamide gel electrophoresis immediately.

然后采用原位杂交方法，对45例骨肉瘤标本组织和10例骨纤维结构不良组织中〓基因mRNA进行检测，以了解骨肉瘤标本组织和骨纤维结构不良组织中〓基因在蛋白质水平和mRNA水平的表达情况。

FCM outcome show that osteosarcoma cell DNA become heteroploid and DNA content is abnormal. Compare with normal muscle cell, the G1 phase cell number of osteosarcoma decrease, however the S phase cell number increase significantly.

流式细胞术检测结果显示：骨肉瘤细胞DNA均呈异倍体改变，DNA含量异常，与正常肌肉细胞相比，骨肉瘤G1期细胞数减少，而S期细胞数显著增多。

The diagnostic criteria,dif-ferential diagnosis and histogenesis of the disease were discussed.

目的 研究喉骨肉瘤的临床病理特征，诊断及鉴别诊断，探索该肿瘤的组织来源方法对1例喉骨肉瘤进行组织形态学和免疫组化研究，结合文献对本病的诊断标准、鉴别诊断及组织来源进行探讨。

Objective To investigate the expression ofnm23 protein in soft tissue sarcoma and its correlation with histological grade.

目的 探讨nm23蛋白在软组织肉瘤中的表达及与软组织肉瘤分级的关系。

Objective To investigate the expression of von Willebrand factor in osteosarcoma and its association with pulmonary metastasis.

目的检测骨肉瘤组织中血管性血友病因子的表达，分析vWF的表达与骨肉瘤肺转移的关系。

Methods: By using immunohistochemistry method, the expression of Survivin in osteosarcoma, osteochondroma and normal osseous tissue, and the expression of P-glycoprotein in osteosarcoma was detected.

采用免疫组织化学S-P法检测凋亡抑制基因Survivin在骨肉瘤、骨软骨瘤和正常骨组织中的表达及P-糖蛋白在骨肉瘤中的表达。

In the last twenty years, the treatment of osteosarcoma has been made a great progress.

在过去的20余年里，骨肉瘤的治疗取得了很大的进展，新辅助化疗已成为骨肉瘤治疗的标准模式。

To investigate the expression of livin protein in osteosarcoma and its clinical significance.

目的 探讨凋亡抑制因子Livin在骨肉瘤中的表达及其与骨肉瘤生物学特性的关系。

Objective: To investigate the expression of integrin α4 in osteosarcoma and significance.

目的研究骨肉瘤组织中integrin α4的表达情况，探讨它与骨肉瘤恶性度、侵袭性的关系及临床意义。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。