聚甲醛

Fabrication of tissue section:after spinal cord injury ld,3d,5d,8d,14d,21d,we sacrificed rats in batch.4% formaldehydum polymerisatum were pured into the cardiac muscle and fixed for 24h.Then obtain the specimen of spinal cord from T_5 to T_(13).Establishing slices,HE dyeing,bcl-2 dectation,terminal doxynucleotidyl transferase-mediated dUTP nick end labeling.On the fourteenth day after spinal cord injury,we detect Gale grading score and tiltboard experiment.

组织切片的制备：脊髓损伤后1d，3d，5d，8d，14d，21d共6个时间段分批处死动物。4%多聚甲醛心肌灌注固定24h，切取每只大鼠T_5～T_(13)节段脊髓组织，常规制成切片，给于HE染色，Bcl-2检测，TUNEL原位末端标记以及伤后第14天检测Gale评分和斜板实验，检测大鼠脊髓损伤后细胞凋亡和脊髓神经功能恢复情况。

It can also used as material to produce suffocating reagent, pesticide, antiseptic and weedicide.

在我国多聚甲醛主要用于生产草甘磷和丁草胺等除草剂，也用于涂料、医药、合成树脂和造纸等领域。

Other materials,such as sulfuric acid, caustic soda and ethanediol, can be purchased form the factory nearby.

聚甲醛生产中其他原料如硫酸、烧碱和乙二醇可在周边生产厂家采购。

METHODS: The rats in the two groups were anaesthetized by intraperitoneal injection of 10 g/L urethane (1 g/kg) and the chest was opened to insert a tube into the ascending aorta for perfusion with 200 mL of the mixture containing glutaric dialdehyde and paraformaldehyde for fixation.

两组大鼠给予质量浓度为10 g/L的乌拉坦1 g/kg腹腔内注射，麻醉后开胸，升主动脉插管，戊二醛与多聚甲醛混合固定液200 mL灌流固定，取嗅球，振荡切片，锇酸后固定，平板包埋，光镜下选取嗅球各层，制备超薄切片，透射电镜下对比观察。

Methods Both immatured and matured oocytes were stained by Mito Tracker Green FM.

方法未成熟卵母细胞和成熟卵母细胞均用Mito Tracker Green FM染色，经多聚甲醛固定后在激光扫描共焦显微镜下观察线粒体的分布。

MATERIALS AND METHODS: hMSCs labeled with enhanced green fluorescent protein were transplanted into the lateral ventricle of neonatal mouse brain. At 0, 9 and 14 days post-transplantation, MICE were sacrificed and their brains were fixed with 4% paraformaldehyde. The engraftment of transplanted cells in coronal section of the recipient mouse brain was examined wider a fluorescent microscope. Indirected immunofluorescent staining was used to detect the expression of neural proteins of these grafted cells.

材料与方法：将标记绿色荧光蛋白的人骨髓间充质于细胞植入到新生3d的小鼠侧脑室中，分别于移植后0d，9d和14d处死受休鼠，取其脑组织，4%多聚甲醛固定后行冠状面冰冻切片，荧光显微镜下观察hMSCs的植入，激光共聚焦显微镜检测植入细胞神经特异性蛋白的表达，定位双重标记的植入细胞。

Methods Rabbits were randomized to three groups. Complex model group were fed with feed containing 0.5% cholesterol for 3 weeks. At the fourth, eighth and twelfth weeks, rabbits were given injection of lipopolysaccharide (1 μg) at auricular artery, cervical part and fold inguen, respectively. Normal group were fed with normal diet, while High cholesterol group were fed with feed confaining 0.5% cholesterol only. After 16 weeks, vascular tissues were analyzed using morphometric and histological methods, and the sera were checked for cholesterol, LDL-C, C-reaction protein and tumor necrosis factor-α. Results The cholesterol and LDL-C of High cholesterol group were increased, compared to those of normal group.

给含0.5%胆固醇的饲料，3周后，分别在第4、8、12周采用耳动脉内、颈部、腹股沟处肌肉注射大肠埃希菌脂多糖，并设立正常组和单纯高脂组。16周后观察兔的一般状态，取血清检查血脂六项、C-反应蛋白和TNF-α，取耳动脉、颈动脉、主动脉弓、胸主动脉、腹主动脉、髂动脉、肝脏，放置4%多聚甲醛中过夜，常规行HE染色，检查血管病变和相关脏器病变情况。

Samples were harvested at 6 week and embedded in paraform, and ground sections of the bone tissue were prepared to observe green fluorescence under laser scanning confocal microscope. Propidium iodide staining of the sections was also performed for observation.

将其植入恒河猴背阔肌的肌袋内，以未转染GFP的BMSC用同样的方法接种块状可吸收HA上，作为对照，术后6周取材，4%的中性多聚甲醛固定，塑料包埋，制作骨磨片PI染色在激光共聚焦显微镜下进行观测。

1Cubic millimeter tissue sampling was fixed with the mix of 1% glutaraldehyde and 4% paraform in 4℃.

利用兔后腿软组织损伤、海水浸泡模型，分别于损伤后浸泡于海水中2小时，浸泡后分别进行各种低渗盐水冲洗，分别于干预处理后1h、3h、6h、8h、12h，在损伤周围0.5cm范围内的肌肉组织中取材，0.5cm3损伤组织用4%福尔马林固定，HE染色，光镜下观察损伤组织的细胞浸润情况，用免疫组化法检测受伤肌肉组织凋亡相关基因Caspase3表达情况；1mm3损伤组织用1%戊二醛一%多聚甲醛混合固定液(pH7.4 ) 4℃固定，电镜观察其细胞凋亡及细胞膜、线粒体等结构的变化；生化检测Na+，K+-ATPase活性，了解其组间的差别。

Experimental materials include CO2 incubator,M199, fetal calf serum,Ⅳ collagenase, 40 g/L of paraform, 20 g/L of cidex,ECGF, mouse-anti-human FⅧ monoclonal antibody(anti-FⅧ-RAg), multi-marked immune analyser(1 420, WALLAC BLCTOR2), centrifuge, inverted microscope, and immunofluorescence microscope, etc.METHODS: Three-step gradient sieve method was used.

实验材料为CO2培养箱，M199，胎牛血清，Ⅳ型胶原酶，40 g/L多聚甲醛，20 g/L戊二醛，ECGF，鼠抗人第八因子单克隆抗体，多标记免疫分析系统(1 420，WALLAC BLCTOR2)，离心机，倒置显微镜，免疫荧光显微镜等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。