聚焦糖

Hungry humans may kill each other over a bottle of water or candy bar much as the recent mirror of those caught without food or water in the New Orleans Astrodome; far better perhaps to remove oneself from such possible dreams to be experienced in the cities and into a new country environment that is more conducive to your continued evolution and the inward focus that it requires.

饥饿的人类可能为了一瓶水或一块糖彼此杀戮，如近期的镜子中那些在新奥尔良阿斯托洛圆顶运动场（于1965年兴建完成，是目前世界上最大的一座室内运动场）受困的人们一样没有食物或水；远为更好的是将自己从在城市中被体验的这种可能的梦想中迁出，并迁入一个更有益于你持续进化以及所需的内在聚焦的新的乡村环境。

The monosaccharides in LBP-4 identified by GC were xylose , galactose , mannose , Rhamnose , glucose and arabinose with the molar ratio of 4. 45: 1. 20: 1. 0: 0.98: 0.90: 0.85. The content of calcium, magnesium, zinc and iron in LBP-4a were 127. 9070mg·g〓, 32. 3953mg·g〓, 0.3845mg·g〓 and 0.2930mg·g〓, respectively. The content of galacturnic acid in LBP-4a was 55. 45% while the content of protein is 3. 80%. According to IR spectra, it contains -COOH group,-NH- roup,-OH group,-NH〓 group and β-D-glucose. The backbone of sugar residues chain in LBP-4a were contained 1→6 indican bond according to periodate oxidation and the results of β-elimination reaction indicated that the chain of polysaccharides and protein were connected by O-linked chemical bond. The photograph of LBP-4a got by laser scanning confocal microscope and scanning electron microscope showed that the LBP-4a combined both with each other and with itself in its crystalloid. The crystalloid was flake and anisotropic.

由LBP-4a的红外光谱图知：LBP-4a中含有-COOH、-OH和-NH〓或-NH-基团，糖链中含有β-D-吡喃葡萄糖；高碘酸氧化实验知LBP-4a糖链中不具有连二羟基和连三羟基结构的单糖残基：具有连二羟基结构的单糖残基：具有连三羟基结构的单糖残基=57.0：16.2：1，六碳糖残基的连接中含有1→6连接的糖苷键；通过β消去反应证明LBP-4a中多糖链和氨基酸间为-O-型连接；由激光扫描共聚焦显微镜和扫描电镜对LBP-4a结晶观察结果推测：LBP-4a结晶中同时存在分子间和分子内结合的现象，LBP-4a分子相互结合时，并不是无规则的相互缠绕形成无规线团结构，而是形成片层结构，并且LBP-4a结晶可能具有各向异性的性质。

Thus,CMG-KALA nanoparticles have great potential as gene vectors for transfection in vitro.2.GS-peptide nanoparticles were synthesized as previous reported method in order to improve the ability of cell penetrating,endosome escaping and nuclear targeting.

通过激光共聚焦、荧光光谱以及红外光谱证明KALA可以成功地连接到CMG纳米颗粒表面；通过静电引力吸附将DNA与CMG-KALA纳米颗粒进行复合，并通过琼脂糖凝胶电泳证明该纳米颗粒对DNA具有很好的保护作用。

PCI2 cells were treated under glucose deprivation as model group. Method Morphological and cytoflowmetric methods (the mitochondrial transmembrane potential; MEF method) were applied to evaluate the protective effect of SAB. The RNA levels of grp75 were measured by RT-PCR and immunocytochemistry were performed to investigate the expression level of PC12 cells under glucose deprivation and treated by SAB.

以PCl2细胞（大鼠肾上腺嗜铬细胞瘤细胞）的无糖培养建立细胞缺糖损伤模型，MTT法检测细胞的存活率；应用流式细胞仪检测亚二倍体细胞的比率及线粒体跨膜电位的改变；应用免疫细胞化学、RT-PCR法研究细胞内应激蛋白grp75的表达变化，激光共聚焦显微镜观察SAB作用下Ca(上标 2+)的变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失