聚合酶

Methods Polymerase chain reaction and restriction fragment length polymorphism was used to identify mdm2 genotypes. The Pearson Chi square test and Woolf statistic method were used to analyze the relative risk and 95% confidence interval in order to find the genetic association between polymorphism of mdm2 gene and symptoms and pathological types of NSCLC.

采用聚合酶链反应和限制性内切酶片段长度多态性方法检测88例NSCLC患者的mdm2基因型，并通过Pearson卡方检验及Woolf方法计算相对风险度及95%可信区间（95%CI），验证mdm2基因多态性与各临床症状及病理类型之间的关联性。

Among the products of these genes are enzymes that interfere with host gene expression and a phage RNA polymerase.

这类基因的表达的产物中，有干扰宿主基因表达的酶和一个噬菌体的RNA聚合酶。

Based on the study of DNA isolation from different samples, this paper applied polymerase chain reaction and PCR-derived technology, and utilized many kinds of known samples and reference materials (such as soybean, maize, rice, potato, and pimiento, etc.) to establish the qualitative, half-quantitative, and quantitative GMO detection method: 1. We established a qualitative PCR screening method for CaMV 35S promoter and Tnos terminator. It must be taken into account that false positive results from screening method.

在DNA提取方法研究的基础上，本文应用聚合酶链式反应(polymerasechain reaction，PCR)及其衍生技术，利用大豆、玉米、水稻、马铃薯、甜椒等多种已知标准样品，建立了多种农作物和植物源性食品的GMO定性、半定量和定量检测方法： 1、在单基因定性检测技术方面，针对现有的商品化GMO中大多数含有花椰菜花叶病毒35S（CaMV 35S）启动子和胭脂碱合成酶基因终止子的特点，本文建立了CaMV 35S启动子和Tnos终止子的定性PCR筛选检测方法。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

为从北京鸭胃肠道中扩增血管活性肠肽基因，根据鸡VIP基因（GenBank登录号X80906），设计了一对简并引物，从北京鸭腺胃、十二指肠和空肠提取总RNA，通过反转录－聚合酶链反应扩增，将从腺胃、十二指肠和空肠中扩增出的产物克隆到pGEM-Teasy载体上，导入大肠杆菌JM109，阳性克隆经双酶切鉴定后测序，将测序结果与鸡和鹅（GenBank登录号为DQ023161）的VIP基因进行同源性比较。

We have demonstrated that the renatured Escherichia colioverexpressed RpoⅠ can reconstitute with Rhizobium core RNA polymerase in vitro. A preparation procedure has been established, which is expected to be useful to further functional study.

我们证明了大肠杆菌表达变复性的RpoⅠ能够与根瘤菌的核心RNA聚合酶在体外重组成全酶，并建立了成熟的制备方案，为以后阐明RpoⅠ的功能奠定了基础。

Replicas of DNA are made when the double helix unwinds as a result of helicase enzyme action and the separated strands serve as templates along which complementary nucleotides are assembled through the action of the enzyme DNA polymerase. The result of the two new molecules of DNA each containing one strand of the original molecule, and the process is termed semiconservative replication.

DNA 的复制在解旋酶解开 DNA 链时开始，解开的链作为模板 DNA 聚合酶的作用下结合相对应的核苷酸，最终形成了两条新的 DNA 的链，每一条的链含有一条链原来的 DNA 单链，因此又叫做半保留复制。

The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示，RSAP技术的引物为2条长度均为18bp的引物，引物的3'端为4~6个碱基的限制性酶切位点序列，接着是12~14个碱基的随机序列，2条引物的限制性位点和随机序列不同：PCR扩增的前5个循环采用35℃的退火温度，随后的35个循环采用48℃的退火温度；在25μL反应体系中，模板DNA用量为20ng，Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L，Tap DNA聚合酶用碳为1.5U，2条引物浓度均为600mmol/L；RSAP技术重复性好，适用性广泛，是一种操作简便、产率中等、稳定可靠的DNA标记技术。

Methods Fifty-three patients diagnosed as chronic hapatitis of C were treated by IFN and Ribavirin for at least 24 weeks. Serum HCV-RNA was detected by fluorescent quantitative PCR. Genotype was determined by Simmonds. Results There was no difference of HCV-RNA levels in different genotypes, as that of ApoB.

临床确诊为慢性丙型肝炎的53例患者，采用干扰素联合利巴韦林抗病毒治疗至少24周，Simmonds酶切分型方法进行HCV基因分型，荧光定量聚合酶链反应法定量检测HCV-RNA，全自动生化分析仪检测血清载脂蛋白B，对不同基因型患者血清HCV-RNA水平和载脂蛋白B的关系进行研究分析。

Methods:The polymerasechain reactionand restriction fragment length polymorphismtechniques were used to detect both Hind III and Pvu IIpolymorphisms in 92 children with simple obesity and 80 healthycontrols.The levels of the plasma lipid,plasma lipoproteins,bodymass index,blood pressure,waistline,chest circumference,gluteal circumference,skinfolds thickness at three measuring points(biceps,subscapular and abdominal wall)were also measured.

应用聚合酶链反应和限制性内切酶片断长度多态性技术，检测了92例单纯肥胖症儿童和80例正常健康儿童的LPL-Hind III与LPL-Pvu II基因多态性，并测定血脂、BMI、血压、腰围、胸围、臀围、以及肱二头肌、肩胛下、腹壁等三个部位的皮褶厚度。

Methods SYBR Green I real-time polymerase chain reaction was used to detect the expression of VEGF mRNA in articular cartilage from 20 OA patients and 10 controls with traumatism. VEGF protein was detected by enzyme-linked immunosorbent assay in articular cartilage.

应用SYBR Green Ⅰ实时定量聚合酶链反应检测20例OA和10例外伤患者关节软骨中VEGF mRNA表达，并用酶联免疫吸附试验测定关节软骨组织VEGF含量。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。