聚合酶

PCR-SSCP was used to screen exons in 45 Wilson disease patients from 39 Chinese families and in 10 normal controls. Nucleotide sequence analysis by autoradiograph and restriction enzyme digest were used to detect the mutant points.

对中国人WD39个家系45例患者的3～20号外显子进行聚合酶链反应-单链构象多态性分析，对有异常者进行序列分析，并通过对该突变点的酶切再次筛选。

Methods:180 HCV patients and 40 healthy controls were selected for the research, and their serum BAFF levels were detected by ELISA. The level of serum HCV-RNA in HCV patients was detected through fluorescent quantitative polymerase chain reaction and analyzed along with the clinical data of HCV patients.

选取180名HCV感染者及40名健康对照，应用酶联免疫吸附法测定血清中BAFF含量，荧光定量聚合酶链反应检测HCV感染者血清中HCV-RNA的水平，并结合HCV感染者临床情况分析。

The expressions of HO-1 and HO-2 mRNA were detected using reverse transcription polymerase chain and the relative amount of CO released into the media was quantitated as carboxyhemoglobin by enzyme linked immunosorbent assay.

通过逆转录聚合酶链式反应和免疫细胞化学法检测HO-1、HO-2 mRNA和蛋白的表达，酶联免疫法检测培养上清液中碳氧血红蛋白含量，四唑氮比色法检测细胞增殖。

DNA were abstracted by means of phenol chloroform, amplification and cataphoresis test of polymerase chain reaction of target DNA,primer sequence of gene segment amplification of 5-hydroxytryptamine (5-HT) 2A receptor gene T102C: 5'-TCT GCT ACA AGT TCT GGC TT-3', 5 '-CTG CAG CTT TTT CTC TAG GG-3'; Reaction system of 50 μL, DNA 0.5 μg,Primer 50 pmol,TagDNA enzyme 2U were added with dNTP to the final concentration of 200 μmol/L.

应用常规苯酚氯仿法抽提DNA，目的DNA的聚合酶链反应扩增及电泳检测，5-羟色胺2A受体基因T102C基因片断扩增的引物序列：5'-TCT GCT ACA AGT TCT GGC TT-3'。5'-CTG CAG CTY TTT CTC TAGGG-3'；采用50 μL反应体系，DNA 0.5 μg，引物50 pmol，TagDNA酶2U加dNTP至终浓度200μmol/L。

After circularization by self-ligation, inverse polymerase chain reaction is used to amplify the integration sequences of the human high metastatic potential hepatocellular carcinoma cell line MHCC97-H genome.

对酶切片断进行自身环化后，运用反向聚合酶链反应对人高转移潜能肝癌细胞株MHCC97-H基因组中整合序列进行扩增。

Objective To establish a sensitive and speedy multiple polymerase chain reaction technique for methicillin resistant staphylococcus aureus and methicillin resistant coagulase negative staphylococci detection.

目的 应用一种敏感快速的多重聚合酶链反应，检测耐甲氧西林的金黄色葡萄球菌和凝固酶阴性葡萄球菌。

Results showed that the specific activity of nitrile hydratase per dry cell weight in engineered Rhodococcus strains driven by Ptac, Pami-1, Pami-2 and PlacZ was 7.5, 6.3, 5.3 and 1.8 times of that in the wild, respectively. It indicated that these promoters could be well recognized by RNA polymerase of Rhodococcus.

结果表明，启动子Ptac、Pami-1(7 bp SD-ATG间隔)、Pami-2(13 bp SD-ATG间隔)和PlacZ在紫红红球菌ATCC 33278中启动表达腈水合酶的比酶活分别是野生菌株的7.5、6.3、5.3和1.8倍，表明这些启动子都可以被红球菌的RNA聚合酶所成功识别。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

应用特异性DNA甲基转移酶抑制剂5-氮-2'-脱氧胞苷（5-Aza-2'-dc，以下简称Aza）及组蛋白去乙酰化酶抑制剂曲古抑菌素A作用人结肠癌细胞株HT29后，用甲基化特异性PCR检测该ING1b基因核心启动子区域CpG岛甲基化情况，用染色质免疫沉淀检测其乙酰化组蛋白绑定的DNA情况，并用逆转录聚合酶链反应检测ING1bmRNA表达。

Pfu DNA polymerase, having 3"-5" exonuclease activity, is one of the DNA polymerases that have the highest fidelity.

Pfu DNA酶具有3'-5'外切酶活性，是保真度最高的DNA聚合酶之一。

Methods In 50 cases of patients after treatment of artificial kidney and 54 healthy volunteers, the prevalence of HHV-7 in peripheral blood mononuclear cell and cell-free viral DNA in plasma was tested by nested polymerase chain reaction assay; the product of PCR was digested by restricted enzyme EcoR I to identify its specificity.

采用巢式聚合酶链反应对50例肾透析病人和54例正常健康人PBMC和血浆标本中HHV-7 DNA进行定性检测；用限制性核酸内切酶EcoRⅠ对PCR产物进行酶切图谱分析以确定扩增产物的特异性。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。