聚合酶

The advantage of supercritical carbon dioxide used as an environmental medium in polymerization and reactions in supercritical carbon dioxide are described. It includes homogeneous free radical polymerizations, precipitation free radical polymerizations, dispersion free radical polymerizations, emulsion and inverse emulsion free radical polymerizations, cationic polymerizations, ring-opening polymerization, melt-phase condensation polymerizations, sol-gel polymerizations, polymer blend synthesis, catalytic chain transfer polymerizations, oxidative coupling polymerization, nitroxide-mediated radical dispersion, atom-transfer radical polymerization, reversible addition-fragmentation chain transfer polymerization, electrochemical polymerization, simultaneous one-pot combination of enzymatic and chemical polymerization, copolymerization of carbon dioxide used as monomer.

评述了ScCO2作为聚合反应介质的优点，以及在ScCO2中可进行的聚合反应类型，包括均相聚合、沉淀聚合、分散聚合、乳液及反相乳液聚合、阳离子聚合、开环聚合、熔融态缩聚、溶液-凝胶聚合、聚合物混合合成、链催化转移聚合、氧化-偶合反应聚合、氮氧自由基可控活性聚合、原子转移自由基聚合、可逆加成-断裂链转移、电化学聚合、原子转移聚合与酶催化开环聚合两种活性聚合在ScCO2中同时聚合、二氧化碳作为原料共聚合等。

The hydrolysates from oat xylan using Xyn Ⅰ Xyn Ⅱ and their mixture for hydrolysis were analysed by HPLC. It was showed that Xyn Ⅰ hydrolyzed mainly the unsubstituted regions of oat xylan, and hydrolytes were high xylooligosaccharides; whereas Xyn Ⅱ exhibited greater catalytic versatility than Xyn Ⅰ and were able to attack substituted regions of the polysaccharide and showed greater activity to low xylooligosaccharides than Xyn Ⅰ to give xylobiose as the main hydrolysate. The purified xylanases were acidic enzymes.

以纯化酶组分及其混合物水解燕麦木聚糖，采用高效液相色谱分析相应的水解产物，结果表明，Xyn Ⅰ降解燕麦木聚糖时，主要降解作用发生在底物中没有取代基的区域，水解产物的聚合度较高；Xyn Ⅱ对底物具有更强的适应性，能降解底物中有取代基的区域，相对Xyn Ⅰ酶组分，Xyn Ⅱ酶组分对低聚合度的木聚糖的活性更高，木二糖为主要降解产物。

Methods 59 male subjects with an age over 70 years old were included in this study. BMD at hip and lumbar 2-4 was measured by dual energy X-ray absorptiometry. CTR genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism.

选取年龄≥70岁的深圳地区男性59例，采用双能X线骨吸收仪测定BMD值，并采用聚合酶链反应－限制性长度多态性技术检测CTR基因型。

Hepatitis B virus; covalently closed circular DNA; polymerase chain reaction

肝炎病毒；乙型；聚合酶链反应；共价闭合环状DNA

The answer was that the former could be far sensitive than the latter, but the former needed complex instruments. The latter could help us to directly judge the order of severity by colors.On the other hand, we took advantage of polymerase chain reactionto amplify the MCP genome of the virus and compare it with other nine published MCP genomes of viruses including CzIV, TIV, CIV, LCDV-1, LCDV-K1, BIV, FV3, GIV, RSIV. The sequencing data showed that the MCP of Cobia iridoviruswas more homologous to the MCP of LCDV-1（89﹪similarity）than that from FV3（47﹪similarity）.

另一方面，为了了解引起海鲡病变之病毒究竟属於何种病毒，我们透过聚合酶链锁反应增幅其主要鞘蛋白基因并加以定序，与其他九种已发表的虹彩病毒之 MCP 核酸及胺基酸序列，包括：CzIV、TIV、CIV、LCDV-1、LCDV-K1、BIV、FV3、GIV、RSIV 之 MCP 进行分析比对，显示 Cobia iridovirus亲缘性和感染大多数硬骨鱼类的 Lymphocystivirus 属的 LCDV-1 最为接近（89﹪相似度），和感染两栖类的 Ranavirus 属的 FV3 亲缘性较低（47﹪相似度）。

Objective: To explore the sensitivity of reverse transcriptase polymerase chain reaction method for detection of Coxsackie virus B3 (CoxB3) infection.

目的：探讨逆转录聚合酶链反应方法在检测柯萨奇病毒B3(CoxB3)感染中的作用。

The treadmill training of T-group rats were continuously trained for 4~6 weeks at the intensity of about 75% VO2max (18??5～24m／min,gradient of 0°,each motion lasting 50 minutes,twice a day). Detect the content of gastrocnemius MHC mRNA by gastrocnemius reverse transcription polymerase chain reaction,and detect the changes of MHC muscle fiber and its size of cross section area through immunohistochemistry. Meanwhile,apply the electric stimulation to determine the maximal tension of isometric contraction of post-training gastrocnemius.

运动训练组大鼠进行连续4～6wk强度约为75％VO2max(18.5～24m／min，坡度为0°，每次运动50min，每天2次)的跑台训练，反转录聚合酶链式反应腓肠肌MHC mRNA含量，以免疫组化方法检测肌球蛋白肌纤维的改变情况及横截面积的大小，同时运用电刺激观察训练后腓肠肌等长收缩最大张力。

The PCR technique was used to amplify the mtDNA D-Loop in 20 individuals of Spanish mackerel collected from the southern coast waters of the Shandong Peninsula. The PCR products were accumulated, purified and sequenced.

采用聚合酶链式反应技术对采自山东半岛南岸水域的蓝点马鲛20个个体的mtDNA D-Loop基因进行扩增，得到了1条大小约为500bp的清晰带。

Methods HLA-A,B typing of 50 patients with EHF were investigated by polymerase chain reaction-sequence specific primer.

方法采用聚合酶链反应-序列特异性引物技术，检测流行性出血热患者的HLA-A、-B等位基因。

Methods The polymorphisms of HLA-DRB1 and-DQB1 alleles were typed by sequence specific primer based on polymerase chain reaction in 50 patients with EHF and 50 normal control subjects.

应用序列特异性引物聚合酶链反应技术检测流行性出血热患者和正常对照组各50例的HLA-DRB1、DQB1等位基因。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。