聚合酶

Methods: A total of 56 pedigreed members were collected. 62 patients with type 2 diabetes mellitus and 82 healthy were collected as controls. HNF-1β gene exon2 from all subjects were examined by polymerase chain reaction-single strand conformation polymorphism.

运用聚合酶链反应-单链构象多态性技术检测2型糖尿病患者62例，健康献血者82例，11个糖尿病家系中56例糖尿病肾病患者的HNF-1β基因exon2片段。

Cerical cytology and biopsy were analyzed for 14 oncogenic HP types using polymerase chain reaction.

用聚合酶链反应分析了宫颈细胞学和活检中的14种致癌HP型。

Objective To investigate the expression of heat shock protein 60 and analyze the significance in cervical carcinoma.

方法应用半定量逆转录聚合酶链反应和免疫印迹分析法检测子宫颈癌手术切除组织57例，正常子宫颈组织57例，观察热休克蛋白60的表达与子宫颈癌发生发展的关系。

The polymerase chain reaction machine is a basic instrumentin molecule biology.

聚合酶链式反应仪是分子生物学的基本仪器。

Methods Allele Specific polymerase chain reaction and Confronting Two-pair primers-PCR techniques were used to determine β_2-AR genetic polymorphisms in 42 patients with asthma,which were treated in The Affiliated Hospital of Inner Mongolia Medical College from 2004 July to 2005 September and 30 healthy controls.

方法对2004年7月至2005年9月内蒙古医学院附属医院收治的42例支气管哮喘患者和30名健康者采用等位基因特异性聚合酶链反应和两对相对引物PCR法(confronting two-pair primers，PCR-CTPP)进行β2-AR基因多态性分析。

Methods ABO, Rh and PRA were studied in ten transplant candidates with one or two-hand defect and potential donors. HLA was confirmed by PRC in these people. Mixed leukocyte culture was performed to determine T-cell alloreactivity between donor and recipient. After surgery, immunosuppressants such as antithymocyte globins, FK506, mycophenolic acid and prednisone were used.

方法对准备接受同种异体手移植的10例单手或双手缺失准备接受异体手移植的患者和潜在的供者进行ABO血型和Rh血型、群体反应抗体检测及混合淋巴细胞培养试验，采用聚合酶链反应方法测定人类白细胞抗原。

Mothods: Cock embryo was used to isolate influenza viruses, and the virus titer was determined through hemagglutination test. The gene was amplified by RT-PCR technique from the isolated strain and sequenced, and the gene phylogenetic tree was analyzed.

采用鸡胚进行病毒分离，采用血凝试验初步确定病毒效价，用逆转录聚合酶链反应法扩增分离株的部分片段后进行克隆和测序，然后进行进化树分析。

Methods: 90 Sprague-Dawley rats were randomly divided into 3 groups: control group, emotional stress group and footshock group.

90只SD大鼠，随机分为3组：空白对照组、足部电击组和情绪应激组，采用反转录－聚合酶链作用技术，检测应激后不同时间点三叉神经节中CGRP mRNA水平的变化。

Methods NPCR method was used to detect Mycobacterium tuberculosis DNA in bioptic tissue from 67 patients with endobronchial tuberculosis, and the results were compared with pathologic examination, brushing smear, sputum smear and culture for Mycobacterium tuberculosis after bronchoscopy.

应用巢式聚合酶链反应对67份支气管内膜活检组织进行结核分支杆菌DNA检测，并与病理检查、刷检涂片、支气管镜检后痰涂片和痰培养结果比较。

The reference standard was any 2 of 5 tests positive: smear, aspirate culture, invasive-specimen PCR, filter paper PCR, and leishmanin skin test.

参考标准是任何5检验时2次呈阳性：涂片，抽吸物培养，侵袭性标本聚合酶链反应，滤纸PCR和利什曼原虫素皮肤试验。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐