聚合酶

Reverse Transcription polymerase chain reaction with RNA PCR Kit,the product of RT-PCR was determined by electrophoresis of agarose gel (100v, 30s ),the result was shoot by GDS gel imaging system, the staps were scaned and quantitated by computer analysis system.

用RNA PCR试剂盒进行逆转录聚合酶链式反应，RT-PCR产物在1.2%琼脂糖凝胶上电泳(100V，30 min)，用GDS凝胶成像系统拍摄电泳结果，各带用计算机图像分析系统扫描定量。

Methods After feeding a high-cholesterol diet to guinea pigs, Oddi′s sphincters were disseced on day 30 and day 60 respectively. We used RT-PCR, western-blotting to evaluate expression level of calponin gene.

饲予豚鼠高胆固醇饮食，分别在30天和60天取出Oddi括约肌，应用逆转录聚合酶链反应和蛋白质免疫印迹法检测动物Oddi括约肌调宁蛋白基因的表达水平。

They found that BMV 1a cooperates with two other viral elements--an enzyme known as 2a polymerase and an RNA sequence called a replication signal--to sequester the virus's genetic material in compartments called spherules.

他们发现：BMV 1a与另外两个病毒成分－一个叫做2a的聚合酶和一个叫做复制信号的RNA序列合作

They foundthat BMV 1a cooperates with two other viral elements--an enzyme known as 2apolymerase and an RNA sequence called a replication signal--to sequester thevirus's genetic material in compartments called spherules.

他们发现：BMV 1a与另外两个病毒成分－一个叫做2a的聚合酶和一个叫做复制信号的RNA序列合作， z36淘范文网

They foundthat BMV 1a cooperates with two other viral elements--an enzyme known as 2apolymerase and an RNA sequence called a replication signal--to sequester thevirus's genetic material in compartments called spherules.

他们发现：BMV 1a与另外两个病毒成分－一个叫做2a的聚合酶和一个叫做复制信号的RNA序列合作

Methods: Forty-three indiviuals who were exposed to arsenic more than 0.05mg/L in well water were chosen and the vein blood and morning urine was collected; The MTHFR C677 polymorphism was analyzed by PCR-RFLP method; Urinary inorganic and methylated arsenic were speciated by high performance liquid chromatography combined with hydride-generation atomic fluorescence spectrometry; Microbiological assay was used to estimate serum folate.

在砷中毒流行区选择饮水史清楚、饮水砷含量高于0.05mg/L的43名居民，采集空腹静脉血并收集晨尿，以聚合酶链反应－限制性长度多态性方法分析MTHFR基因C677T位点多态性，采用高效液相色谱－氢化物发生－原子荧光法检测尿中各种形态的砷，采用微生物法测定血清中叶酸水平。

Short interspersed elements are widespread among eukaryotic genomes and are moderately repetitive DNA sequences. These molecules range from 70-500bp in length. There are usually 103-105 copies of a SINEs family in a eukaryotic genome. The evidences suggested that SINEs have proliferated by retrotransposition.

短散在核重复序列(short interspersed elements SINEs)是广泛分布于真核生物基因组内的中度重复序列，分子长度在70-500bp，在基因组内的拷贝数为103-105；SINEs在基因组内以返转座的方式进行增殖，由RNA聚合酶III转录成RNA，再反转录成cDNA插入到基因组内。

Methods Marrow-derived mesenchymal stem cells were isolated and purified from mouse femoral bone and shinbones using differential adherent methods. Cells at the third passage were induced by 20% FBS in conditioned medium, conditioned medium alone, 20% FBS or 10% FBS alone respectively. Mouse aortic smooth muscle cells were cultured as the positive control. Levels of mRNA and protein expression of myoeardin and several smooth muscle cells marker genes were determined by immumofluorescenee, RT-PCR and Western blot before and 3, 7, 10, 14d after the induction. The presence of smooth muscle myofilaments was detected by using transmission electron microscope.

采用伞骨髓贴壁法分离骨髓问充质干细胞，CM联合20%FBS诱导骨髓间充质干细胞，同时设立持续10%FBS、单20%FBS及单CM诱导下的骨髓间充质十细胞对照组和SMC阳性对照组，分别于诱导前及诱导3、7、10和14 d时观察细胞形态的变化，并在相应的时间点用免疫荧光法、逆转录聚合酶链反应法、Western blot半定量分析法榆测myocardin以及SMC表面各种标志基因的表达变化，用透射电镜检测诱导后细胞内肌丝存在以此来证实诱导分化成功。

The product would be developed for primary manifestations of all 4 major serotypes of Dengue virus infection in adults and children.product profile

这种产品会被开发成同时针对成人和儿童登革热患者的抑制四种主要登革热血清型的小分子聚合酶。

After 1,4, 7, 10, 14 days of exposure, eight rats of each group were sacrified to assess lung histopathologic changes.

两组分别于暴露满1、4、7、10和14d时，每组取动物8只，行肺组织病理学检查，半定量逆转录聚合酶链反应测定COXI、COXII和COXIIImRNA的表达。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐