聚合酶

Observe the change cardiac rhythm and rate;The expression of GimRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Observe the change cardiac rhythm and rate;The expression of Gi-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Methods: Arrhythmia was induced with cordilox, and acupuncture two-aide "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针异搏定所致的缓慢性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白 mRNA的相对含量。

Methods: tachyarrhythmia was induced with aconitine, and acupuncture two-side "Neiguan" by electro-acupuncture later. Observe the change cardiac rhythm and rate; The expression of G-mRNA in bulbus medullae was detected by using reverse transcription polymerase chain reaction.

电针乌头碱所致的快速性心律失常模型大鼠双侧&内关&穴，观察针刺前后心律变化；用半定量逆转录聚合酶链反应方法测定延髓中抑制性G蛋白mRNA的相对含量。

Woodchucks . The HBV genome has four genes: pol, env, pre-core and X that respectively encode the viral DNA-polymerase, envelope protein, pre-core protein (which is processed to viral capsid) and protein X. The function of protein X is not clear but it may be involved in the activation of host cell genes and the development of cancer.

乙型肝炎病毒基因组有四个基因：波兰，环境，前核心和X ，分别编码病毒DNA聚合酶，包膜蛋白，前核心蛋白和蛋白质十大功能蛋白X是尚不清楚，但它可能参与激活宿主细胞的基因和癌的发展。

Monocyte chemotactic protein-3 (MCP-3) interacts with multiple leukocyte receptors: bin-ding and signaling of MCP-3 through shared as well as unique receptors on monocytes and neutrophils. Eur J Immunol, 1995; 25:2612-261711 Warrens AN , Jones MD, Lechler RI, et al.

实验中发现，重叠延伸拼接PCR过程中碱基与模板的错配率要明显高于常规PCR反应，即使使用保真性较好的聚合酶也不能避免错配的发生，而可能导致碱基序列的点突变。

The number of 5-Br deoxy uridine monophosphate and nestin in subventricle zone and dentate gyrus of rats at 3, 5, 7, 14, 21 and 30 days after MCAO were detected by immunohistochemistry and the bFGF mRNA were detected by RT-PCR at the same time.

免疫荧光法检测各组大鼠缺血再灌注损伤后第3、5、7、14、21、30天病灶侧侧脑室下区、海马齿状回区5-溴脱氧尿嘧啶核苷和Nestin的变化，逆转录聚合酶链式扩增方法检测相应时间点病灶侧碱性成纤维生长因子信使核糖核酸的表达。

In exploring the mechanism underlying the decreased transcription of SPARC in NPC cells, we found that the transcription factor SRY (sex-determining region Y)-box 5 (SOX-5) is upregulated in NPC cells.

再经由定量聚合酶连锁反应(Quantitative RT-PCR)以及西方吸渍法的确认分析，发现 SPARC 基因在鼻咽癌细胞中的表现量显著减少。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

As RNA polymerase pushes forward along the double helix, it generates positive supercoils ahead and leaves negative supercoils behind.

11.19 转录可能能导致在RNA聚合酶前的DNA片断缠绕更紧密，在其后的变得缠绕的不那么紧密。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐