聚合酶

Methods:Polymerase chain reaction was used in the study of GSTP1 genotype, than the discrepancies of GSTP1 genotype frequences between 56 UC patients and 120 healthy controls were anatysed by x2 test.

用聚合酶链反应技术检测56例溃疡性结肠炎和120例健康人群的GSTP1基因型，采用x2检验，分析比较GSTP1基因型频率在UC组和健康对照组之间的分布差异。

The typical spot symptoms on fruit would decrease the production and merchandise of tomato in the heavy rain of June and July; therefore, the key period for disease control is after rainy season. Quick identification on pathogen was using PCR. 1476bp band was amplified by primer P3、P4of bacterial speck. By using primer CMM5、CMM6 for bacterial canker, 614bp fragment was amplified. These PCR tests provided important foundation for pathogen identification and disease diagnoses. Using primer RST65 and RST69 for bacterial spot, expected 420bp band was not amplified.

通过聚合酶链式反应对病原进行快速鉴定，证明加工番茄细菌性斑点病用引物P3、P4可以得到一条大小为1476bp的特异性条带，番茄细菌性溃疡用CMM5、CMM6可以扩增出一条大小为614bp的特异性条带，这为病害的快速诊断打下了基础，但利用引物RST65和RST69对番茄细菌性疮痂病进行鉴定，没有得到预期420bp的目标条带，故对细菌性疮痂病的快速检测还有待继续研究。

Objective To detect the DNA of Neisseria gonorrhoeae in clinical genitourinary specimens by the real-time fluorescence quantitative Polymerase Chain Reaction , and to provide a reliable support for the clinical diagnosis and treatment of gonorrhoea.

目的 应用实时荧光定量聚合酶链反应技术准确定量检测临床标本中淋病奈瑟菌基因，同时与金标准—细菌培养法比较其敏感性和特异性，为临床淋病的诊疗提供依据。

Groups were divided as follows: diabetic group, 1,25-2D3 treated group [1,25-2D3 group] and control group. At the 12 week, haematuria and proteinuria were determined and the kidneys were removed by histopathology, immunostaining, and the expression of Nephrin mRNA and protein in renal cortex were detected by reversed transcription-polymerase chain reaction.

建立糖尿病大鼠模型，实验12周末观察各组血尿、蛋白尿、肾组织光镜电镜、免疫组织化学，采用反转录聚合酶链反应检测其肾皮质Nephrin mRNA及蛋白的表达。

Methods: Male Sprague-Dawley rats were subjected to lateral fluid percussion brain injury of moderate severity. bax and bcl-xL mRNA and protein expression was detected by RT-PCR an d immunohistochemistry. In addition to morphological evidence of apoptosis, TUNE L histochemistry was used to identify DNA fragmentation in situ under both l ight and electron microscope, whereas characteristic internucleosomal DN A fragm entation of apoptosis was demonstrated by DNA gel electrophoresis.

在液压脑损伤模型中，应用逆转录聚合酶链反应、免疫组化分别检测大鼠脑创伤后不同时程bax和bcl-xL表达；采用凋亡原位末端标记、电镜超微结构、DNA凝胶电泳观察脑创伤后细胞凋亡的形态和生化特征。

Breast tissues were taken at surgery from patients with breast adenoma, nodule of lobuli glandulae mammariae and breast cancer. Normal samples adjacent to breast cancer were used as control. NIS mRNA and protein were assessed in these samples by reverse transcription polymerase chain reaction , Western blot and immunohistochemistry methods.

1。乳腺组织取自乳腺纤维瘤、乳腺小叶增生以及乳腺癌患者，另取癌旁正常乳腺组织作为对照，分别运用半定量逆转录聚合酶链反应、Western Blot方法和免疫组织化学技术检测NIS的基因和蛋白表达情况。

Objective To study the diagnotic value of combining PCR with IS986 gene probe marked with long-arm light-sensitive biotin.

目的 研究聚合酶链反应联合长臂光敏生物基因探针对浆膜腔积液性质的诊断价值。

The long specific DNA probes were synthesized by polymerase chain reaction in the highly specific gene sequences of above 10 bacteria. The species-specific probes synthesized were inoculated on a nitrocellulose filter and hybridized with template DNA labeled with long-arm photosensitive biotin. The signal of hybridization reaction was observed to detect which bacteria infected. The sensitivity and specificity of the method were studied.

在这10种病原菌高度特异的基因序列内利用聚合酶链反应合成上述10种细菌特异的长链DNA探针，并将合成的特异探针固定在硝酸纤维素膜上，与用长臂光敏生物素标记后的模板DNA进行斑点杂交反应，通过观察杂交信号判断存在何种细菌感染，同时研究该方法的敏感性和特异性。2。

METHODS By nested polymerase chain reaction, the inactivation efficacy of three different concentrations of iodophor, potassium permanganate, Lysol,"84" disinfectant and jingxin liquid disinfectant was detected after TTV having been disinfected for 10, 30, 60 and 90 minutes by these disinfectants respectively.

采用巢式聚合酶链反应技术，检测 3种不同浓度的碘伏、高锰酸钾、煤酚皂液、&84&消毒液和金星消毒剂，对 TTV DNA分别消毒处理 10、30、6 0 min和 90 m in的灭活效果。结果碘伏在常规使用浓度(0 。5 %～ 1%)对 TTV DNA灭活效果好；高锰酸钾、来苏尔、&84&消毒液和金星消毒剂在高浓度下可灭活 TTV DNA ，而常规使用浓度对 TTV DNA灭活效果不好。

The established event-specific real-time PCR detection systems in this study are suitable for the identification and quantification of these GM maizes.

再辅以即时聚合酶链锁反应( real-time PCR )检测基因改造玉米含量，将有助於建立基因改造玉米产品标示管理。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐