聚合酶

To explore the reciprocal effects of host-tumor interaction, we developed a system to assess the gene expression patterns and the diversity of gene expression of ERK and PI3K pathway in CAOV3 human ovarian cancer cells and primary carcinoma-associated fibroblasts cocultured with each other for 48 hours with RT-PCR.

方法1。采用逆转录聚合酶链反应的方法分别检测交互作用48小时后的卵巢癌相关成纤维细胞与卵巢癌细胞中基因表达的改变，CAFs及CAOV3细胞外信号调节激酶通路的ERK，PI3K基因表达的变化；2。

Methods To detect GPⅢ a P1^A polymorphism in 100 cerebral thrombosis patients and 100 healthy collators with polymerase chain reaction restriction fragment length polymorphismtechnique.

方法运用聚合酶链反应-限制性片断长度多态性分析技术检测100例脑血栓患者及100例健康对照者GPⅢa的P1^A的多态性。

Several studies, both in vitro and in vivo, have demonstrated that HBV replication can be inhibited in transfected cells by synthetic siRNA duplexes and also Pol III-derived short hairpin RNA sequences.

多个体内体外研究证实：在转染细胞中的乙肝复制可被合成的小分子干扰RNA双链体或针对聚合酶III的短发夹RNA序列所抑制。

Results The two patients with primary dystonia were found to have the gene mutation of SCA3,the CAG repeat were 80 and 75 respectively.

方法采用聚合酶链反应方法对一家父子2例原发性肌张力障碍患者及其21名家系健康成员SCA1~3型基因CAG重复数进行检测。

Methods Peripheral blood eosinophil MBP mRNA levels were measured in 40 patients with asthma and 20 normal controls by semi-quantitative RT-PCR.

方法采用逆转录聚合酶链反应技术对40例哮喘患者及20例正常对照者的MBPmRNA表达水平进行半定量分析。

Nucleoside analogs can decrease virus production by inhibiting the viral polymerase; however, complete clearance by these drugs is not common because of the persistence of the HBV episome.

核苷类似物可能通过禁止病毒聚合酶减少病毒生产；然而，完全清除由这些药物不是共同的由于HBV的坚持episome。

Objective To explore the subcellular localization of HBVDNAPTP1 transactivated by hepatitis B virus DNA polymerase with green fluorescent protein expression system in eucaryotic cells, and predict the protein structure by bioinformatics.

目的 利用真核细胞绿色荧光表达系统分析乙型肝炎病毒DNA聚合酶反式调节蛋白HBVDNAPTP1的亚细胞定位，并用生物信息学方法对其结构进行分析。

The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

DNA相互作用的情况，而且可以检测体内的DNA构象的变化，连接介导的聚合酶链反应(Ligation-Mediated polymerase chain Reaction，LM-PCR)的发明，使得体内足迹研究的敏感性和特异性大大提高，有力地促进了体内足迹技术在真核基因表达调控研究方面的应用。

In vivo footprinting is a method of studying DNA-protein interaction. It can reflect the authentic status of DNA-protein in vivo, and can also detect the change of DNA conformation. The introduction of Ligation-Mediated PCR greatly improves the sensitivity and specificity of in vivo footprinting study, and has facilitated the executation of in vivo footprinting in the regulation of eucaryotic gene expression.

体内足迹法是一种研究DNA-蛋白质相互作用的方法，它能真实反映体内蛋白-DNA相互作用的情况，而且可以检测体内的DNA构象的变化，连接介导的聚合酶链反应(Ligation-Mediatedpolymerase chain Reaction，LM-PCR)的发明，使得体内足迹研究的敏感性和特异性大大提高，有力地促进了体内足迹技术在真核基因表达调控研究方面的应用。

Methods: Polymerase chain reaction was used in the study of GSTP1 genotype, than the discrepancies of GSTP1 genotype frequences between 56 UC patients and 120 healthy controls were anatysed by χ^2 test.

用聚合酶链反应技术检测56例溃疡性结肠炎和120例健康人群的GSTP1基因型，采用χ^2检验，分析比较GSTP1基因型频率在UC组和健康对照组之间的分布差异。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。