聚合酶

It replicates by an RNA-dependent RNA replication mechanism, which is carried out by a cellular enzyme.

这个病毒带有圆形的RNA，是在自然界很奇特的RNA，特别是它的复制需要用细胞的RNA聚合酶。

As RNA polymerase moves along the DNA template, it unwinds the duplex at the front of the bubble, and rewinds the DNA at the back. The length of the transcription

当RNA聚合酶沿DNA模板移动时，它打开泡前的DNA双螺旋，并在复链点使DNA复原，转录泡的长度随增长反应阶段而异，在12-20bp之间，但其中的RNA-DNA杂交体区域比这个长度要短。

The NS5B protein's RdRp activity is distinct when use different genetic-coming 3'terminuses of negative RNA strand as templates.

NS5B蛋白对HCV 1a、1b和2a型负链3'末端RNA模板具有不同强弱的聚合酶活性。

The DNA sequences corresponding to the 3'terminuses of HCV genotype 1a, 1b and 2a negative RNA strand were cloned to the transcription plasmid pGEM3Zf.

以1a型负链3'末端RNA为模板，NS5B1和NS5B2为聚合酶，进行体外的RNA合成反应，斑点杂交和RT-PCR检测产物，确定纯化的蛋白具有RdRp活性。

The 3'terminuses of negative RNA strand from 1a, 1b and 2a were prepared via in vitro transcription, using these RNA as temlates and the two purified NS5B proteins as polymerase respectively we conducted the RdRp assay in vitro.

分别以体外转录的HCV 1a、1b和2a型负链3'末端RNA作为模板RNA，NS5B1和NS5B2为聚合酶进行体外RdRp反应，Northern blot检测产物合成情况。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清，进行以下检测：(1)原子吸收法测血清锌浓度；(2)测胸腺、脾脏脏器重量并与大鼠体重比较，计算脏器指数；(3)HE染色对胸腺、脾脏进行病理观察；(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡，并通过图像分析进行比较；(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达；(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达；(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

Each RNA polyerase transcribes only one strand of a double-stranded DNA template.

每种RNA聚合酶只转录双链DNA模板中的一条链。

The RNA polymerase unzips a small portion of the DNA helix exposing the bases on each strand.

RNA聚合酶解开一小段DNA螺旋，使碱基在它们各自的链上暴露出来。

In order to determine the etiology of pseudocondyloma of vulvae and its relation with condyloma acuminata,42 cases of PCV were studied by polymerase chain reaction,histopathology and transmitted electron microscopy.

为了明确女阴假性湿疣的病因及与尖锐湿疣有无相关性，本文采用聚合酶链式反应技术以及病理、电镜等方法对42例PCV的病因及诊断进行了研究。

Pseudocondyloma of vulvae ; etiology ; diagnosis ; human papillomavirus ; polymerase chain reation

女阴假性湿疣；病因学；诊断；人乳头瘤病毒；聚合酶链式反应

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐