聚合酶

Tuberculosis ; cutaneous Nodular dermatoses Polymerase chain reaction

结核；皮肤；结节性皮肤病；聚合酶链反应

Four pairs of primes were synthesized to amplify Salmonella genus special genes such as hut gene(495 bp), hilA gene(490 bp), invA gene(284 bp) and hns gene(152 bp) of Salmonella, respectively. The PCR reaction condition was optimized.

近年来，分子生物学方法如聚合酶链式反应在沙门氏菌的检测上已得到较好应用[1-6]，大大缩短了检测时间，但各报道中所用的DNA提取方法、引物、PCR反应条件各异。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

Normal mammalian guanylate kinase metabolizes the monophosphate to the diphosphate form and then to the triphosphate form .

GCV-TP对细胞具有剧毒性，可抑制DNA聚合酶的活性，并可掺入到DNA链的末端作为终止剂。

Methods The expression of CD44 mRNA was examined in cancerous tissues and adjacent noncancerous mucosae from 26 patients with colorectal cancer and in colorectal polyps from 7 patients by reverse transcription-polymerase chain reaction and Southern blot hybridization.

应用逆转录、多聚合酶链反应和Southern杂交技术，检测26例大肠癌患者癌组织、正常大肠粘膜组织和7例大肠息肉CD44基因mRNA表达。

MMP3, TIMP-1 expression of the rats myocardia are measured with RT-PCR.

采用半定量逆转录聚合酶链反应的方法测定大鼠心肌组织MMP-3、TIMP-1的表达。

Methods Using polymerase chain reaction-restriction fragment length polymorphism method, we detected 63 samples taken from 63 cases (including 36 invasive squamous carcinoma, 7 carcinoma in situ, 20 papillomas) for 248 point mutations in p53 gene.

采用聚合酶链反应――限制性片段长度多态性（polymerase chain reaction-restriction fragment length polymorphisms，PCR-RFLP）方法，对63例患者的送检标本（包括36例浸润性鳞状细胞癌，7例原位癌，20例乳头状瘤）中p53基因248位点的突变进行检测。

Ob j ect i ve To reveal the relationship between PON-1 Q/R polymorphism, serum PON-1 activity and T2DM complicated by CAD in Chinese Han ethnic group and to provide objective evidence in the prophylaxes and management of T2DM complicated by CAD.Methods The subjects of the study consist of four groups:(1) T2DM group (22 men and 14 women, mean age 56 ?8 years);(2) CAD group (35 men and 6 women, mean age 60 ? 13 years);(3) T2DM complicated by CAD group(14 men and 13 women, mean age 66 ?9 years);(4) normal-control group(21 men and 12 women, mean age 57 + 11 years).

目的与方法本研究应用聚合酶链式反应—限制性片段长度多态性方法鉴定天津地区汉族人单纯T2DM患者36例、单纯CAD患者41例、T2DM合并CAD患者27例和正常对照组33例的PON-1 Q／R基因多态性的基因型，并测定血清PON-1活性，探讨PON-1在T2DM并发CAD中的作用，为该病的防治提供客观依据。

Methods:With unmobile-phase enzymatic HPLC,48 persons'15 serum bile acids were quantitated.

采用高效液相色谱反相聚合酶柱分离测定血清中15种胆汁酸的含量，共48例。

The PCR method can be used for reduplicating a segment of a DNA molecule, e.g.

等发明了具有划时代意义的聚合酶链反应。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。